Navegando por Autor "Azevedo, Ricardo Antunes"

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Antioxidative responses of cell suspension cultures of two Coffea arabica varieties to low aluminum levels at pH 5.8
(Instituto de Pesquisas Ambientais, 2012) Bottcher, Alexandra; Nobile, Paula Macedo; Martins, Paula Fabiane; Conte, Fábio Frangiotti; Azevedo, Ricardo Antunes; Mazzafera, Paulo
The effects of aluminum (Al) on the activities of antioxidant enzymes and ferritin expression were studied in cell suspension cultures of two varieties of Coffea arabica, Mundo Novo and Icatu, in medium with pH at 5.8. The cells were incubated with 300 μM Al3+, and the Al speciation as Al3+ was 1.45% of the mole fraction. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) were increased in Mundo Novo, whereas glutathione reductase (GR) and guaiacol peroxidase (GPOX) activities remained unchanged. SOD, GR, and GST activities were increased in Icatu, while CAT activity was not changed, and GPOX activity decreased. The expression of two ferritin genes (CaFer1 and CaFer2) were analyzed by Real-Time PCR. Al caused a downregulation of CaFER1 expression and no changes of CaFER2 expression in both varieties. The Western blot showed no alteration in ferritin protein levels in Mundo Novo and a decrease in Icatu. The differential enzymes responses indicate that the response to Al is variety-dependent.
Simple procedure for nutrient analysis of coffee plant with energy dispersive X-ray fluorescence spectrometry (EDXRF)
(Escola Superior de Agricultura "Luiz de Queiroz", 2013-07) Tezotto, Tiago; Favarin, José Laércio; Paula Neto, Ana; Gratão, Priscila Lupino; Azevedo, Ricardo Antunes; Mazzafera, Paulo
Nutrient analysis is used to estimate nutrient content of crop plants to manage fertilizer application for sustained crop production. Direct solid analysis of agricultural and envi- ronmental samples by energy dispersive X-ray fluorescence spectrometry (EDXRF) was chosen as alternative technique to evaluate the simultaneous multielemental quantification of the most important essential elements in coffee (Coffea arabica L.) plants. Inductively coupled plasma atomic emission spectrometry and certified reference materials made from leaves were used to calibrate and check the trueness of EDXRF method for the determination of the concentration of several nutrients in coffee leaves and branches. Fluorescence spectrometry proved to be ad- vantageous and presented low cost as loose powder samples could be used. Samples collected from a field experiment where coffee plants were treated with excess of Ni and Zn were used to verify the practical application of the method. Good relationships were achieved between certi- fied values and data obtained by EDXRF, with recoveries ranging from 82 to 117 %.