Navegando por Autor "Lopes, Ueder Pedro"

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Effect of foliar application of potassium silicate on the progress of coffee leaf rust
(Sociedade Brasileira de Fitopatologia, 2013) Lopes, Ueder Pedro; Zambolim, Laércio; Souza Neto, Pedro Nery; Souza, Antônio Fernando; Capucho, Alexandre Sandri; Rodrigues, Fabricio de Ávila
In order to evaluate the effect of potassium silicate spray on the control of coffee leaf rust and coffee yield, an experiment was conducted during the 2005/2006 and 2006/2007 growing seasons on Coffea arabica cv. Catuaí Vermelho IAC 144, in a location situated at an elevation of 850 m in Coimbra, Minas Gerais, Brazil. The treatments consisted of: 1 - potassium silicate; 2 - potassium silicate + copper hydroxide; 3 - copper hydroxide; 4 - epoxiconazole formulated with pyraclostrobin and 5 - control treatment. Six applications of potassium silicate and copper hydroxide were made twice per month from December to March of each year. Two applications of the systemic fungicide were done in December and March of each year. Potassium silicate alone or in combination with copper hydroxide was not efficient in reducing coffee leaf rust incidence and increasing yield under high incidence of coffee leaf rust.
Prospecting of endophytic fungi producing volatile organic compounds: taxonomy, identification of volatiles and potential uses for the biological control of postharvest diseases
(Universidade Federal de Viçosa, 2017-02-17) Gomes, André Angelo Medeiros; Pereira, Olinto Liparini; Lopes, Ueder Pedro; Queiroz, Marisa Vieira de
In the search for alternative strategies to control of postharvest diseases in fruits and vegetables aimed at reducing the use of fungicides, was conducted a prospection to endophytic fungi capable of emitting volatile compounds with antimicrobial properties. Through parallel growth technique, directed towards the isolation of fungi producing volatile antimicrobials, endophytic isolates of coffee and carqueja plants were obtained and in preliminary test inhibited the growth of Aspergillus ochraceus by the emission of volatile compounds. The presence of at least three distinct species of Muscodor was verified, and a new taxon of Simplicillium by clarifying the identity of the endophytic isolates obtained through comparative studies of morphological structures, BLAST of gene sequences and phylogenetic analyzes. M. yucatanensis, Muscodor sp. and M. coffeanum isolates were obtained from coffee branches, the latter also isolated in leaves of carqueja. The antimicrobial activity of these isolates was demonstrated against Aspergillus species frequently associated with coffee beans by in vitro mycofumigation. In addition, mycofumigation with M. coffeanum CDA 741 inhibited the growth of A. ochraceus inoculated in coffee beans. The compounds present in the volatile mixture emitted by each isolate were identified by solid-phase micro-extraction gas chromatography and mass spectroscopy. Simplicillium sp. CDA 734, a new species of endophytic Cordycipitaceae from coffee plants, will be proposed through comparative studies of its morphology combined with phylogenetic analyzes. Simplicillium sp. CDA 734 inhibited the growth of A. ochraceus, A. tubingensis, A. sydowii and A. niger by mycofumigation. Among the compounds identified in the volatile mixture emitted by Simplicillium sp. CDA 734, 1-Propanone, 1-(5-methyl-2-furanyl)-, Cyclopropane, 1- ethoxy-2,2-dimethyl-3-(2-phenylethynyl)-, and 2-Propenoic acid, 3-(2-formyl-4- methoxyphenyl)-, ethyl ester, (E)- were those with the highest percentage of peak per area. In mycofumigation of organic strawberries inoculated with Colletotrichum acutatum e Botrytis cinerea, M. coffeanum CDA 739 decreases 100 and 81% the incidence of anthracnose and gray mold in the fruits, respectively. Mycofumigation through sachets containing rye grains colonized by M. coffeanum CDA 739 significantly reduced the incidence of anthracnose in fruits inoculated with C. acutatum, making possible its use in large-scale to control postharvest strawberry diseases through mycofumigation.
Silicate slag combined with tebuconazole in management of brown eye spot in coffee
(Editora UFLA, 2013-04) Lopes, Ueder Pedro; Zambolim, Laércio; Lopes, Uilton do Nascimento; Rios, Jonas Alberto; Duarte, Henrique Silva Silveira; Ribeiro Júnior, José Ivo
The aim of this trial was to evaluate silicon absorption and the effect of silicate slag application combined with triazole fungicide in control of brown eye leaf spot under greenhouse conditions, using coffee seedlings of the cultivar Catuaí Vermelho 144. A completely randomized 5x2 experimental design was used, with and without application of the fungicide tebuconazole, with eight replications. The mixtures (M) used on the soil were defined as based on the liming needs of the soil: M 1 - 0% de silicated slag (slag) and 100% limestone (lim); M 2 - 25% slag and 75% lim; M 3 -50% slag and 50% lim; M 4 - 75% slag and 25% lim; M 5 - 100% slag, with or without application of the fungicide tebuconazole. The fungicide tebuconazole (0.5 g of a.i./L) was applied on coffee seedlings through five pair of leaves. Twenty-four hours after fungicide application, each coffee seedling was inoculated with a conidial suspension of 2.0 x 10 5 /mL. The severity and the defoliation caused by disease were evaluated every five days from 20 to 45 days after inoculation. With the severity and defoliation data, the area under the brown eye spot disease progress curve (AUCPBES) and the area under the defoliation curve (AUCPDESF) were calculated. The application of increasing doses of silicate slag on the soil did not reduce AUCPBES and AUCPDESF, and no increase in Si content in the root, stem or leaf was observed. Tebuconazole reduced AUCPBES and AUCPDESF.