Navegando por Autor "Pereira, Diego de Sousa"

Agora exibindo 1 - 3 de 3

Association between the artificial aging test and the natural storage of coffee seeds
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2018) Fantazzini, Tatiana Botelho; Rosa, Sttela Dellyzete Veiga Franco da; Pereira, Cristiane Carvalho; Pereira, Diego de Sousa; Cirillo, Marcelo Ângelo; Ossani, Paulo César
The accelerated aging test is recognized as an efficient method for evaluating the vigor of seed lots and for estimating their storage potential. Thus, this work aimed to evaluate the association between artificial aging and natural storage of coffee seeds, through the correlation factor analysis. Seeds of four cultivars of Coffea arabica L. (Catuaí Amarelo, Arara, Catiguá, and Mundo Novo) and one of Coffea canephora Pierre (Apoatã) were used. Part of the newly-harvested seeds were aged in a growth chamber under controlled temperature and relative humidity conditions (42 ºC and 100% RH) for periods of 0, 4, 6, 8, and 10 days. The other part of the seeds was stored in tri-wall paper packaging for a period of 2, 4, and 6 months in a non-climate-controlled environment. Artificial aging allows predictions on the storage potential of coffee seeds, although the artificial aging periods depend on the cultivars.
Effect of physiological priming on stored coffee seeds
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2021) Penido, Amanda Carvalho; Rezende, Édila Maria de; Pereira, Diego de Sousa; Reis, Venícius Urbano Vilela; Rocha, Debora Kelli; Oliveira, João Almir
The physiological priming has been used to standardize and increase the speed of development of seed lots. The use of this technique is essential in coffee cultivation, as the crop has low longevity associated with slow and uneven germination. The objective was to evaluate the effect of physiological priming on the physiological quality of coffee seeds stored with different water contents. Seeds of two cultivars of Coffea arabica were used: cv. Catuaí Vermelho IAC144 and Topázio MG1190. A portion of the seeds were dried in the shade until reaching 12% moisture, and the other did not undergo drying. The seeds were stored in a cold chamber at 10 °C for nine months. Every three months, the physiological priming technique was performed with subsequent evaluation of the physiological quality by germination tests, root protrusion, normal seedlings at fifteen days, strong normal seedlings, seedlings with expanded cotyledonary leaves and seedling dry weight. Seeds that did not undergo drying subjected to the physiological priming technique maintained physiological quality after nine months of storage. Physiological priming was detrimental to dry seeds stored for nine months.
Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds
(Editora UFLA, 2020) Oliveira, Leonardo Araujo; Souza, Genaina Aparecida de; Silva, Bruno Tavares; Rocha, Antônio Augusto Gomes; Picoli, Edgard Augusto de Toledo; Pereira, Diego de Sousa; Donzeles, Sérgio Maurício Lopes; Ribeiro, Marcelo de Freitas; Ferreira, Williams Pinto Marques
The endosperm of coffee beans is an important structure which is composed of different reserve compounds. In the present study, we followed anatomical features during the reserve mobilization of Coffea arabica L. cv ‘Catuaí’ seed samples, harvested at 20 different sites, after 0, 3, 6, 12, 24, 30 and 45 days of imbibition. Seed samples were properly stored for enzyme activity assessment and fixed in FAA solution (formaldehyde, acetic acid and ethanol, 1; 1; 18) and kept in ethanol 70% solution, for histochemical analysis. Alternatively, samples for phenolic compounds histochemical test were fixed in Ferrous sulfate in formalin. Fixed samples were cross-sectioned for detection of starch, lipids, essential oils, oleoresins, proteins, phenolic compounds, neutral polysaccharides, alkaloids, β-1,3 and β-1,4 glucans, and lignin. Overall, based on histochemical tests and enzyme activity, seed reserve mobilization was similar among the evaluated altitudes and mountainsides. During soaking, there is an intense consumption of reserve compounds, starting at the region close to the embryo. Reserve mobilization intensifies after root protrusion, from 12 days of soaking. The intensification of the reserve consumption concomitant with an increased activity of MDH is observed at the first 12 days, whereas SOD showed higher expression after 30 days of soaking. At the 30th day, little reserve is observed in the endosperm. At 45th day, cotyledon leaves are expanded, and the root system, constituted by the taproot and lateral roots, is well established.