Navegando por Autor "Queiroz, Marisa Vieira de"

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Enzyme Production by Induratia spp. Isolated from Coffee Plants in Brazil
(Instituto de Tecnologia do Paraná - Tecpar, 2020) Monteiro, Mônica Cristina Pereira; Tavares, Dérica Gonçalves; Nery, Eduardo Mateus; Queiroz, Marisa Vieira de; Pereira, Olinto Liparini; Cardoso, Patrícia Gomes
Endophytic fungi belonging to the genus Muscodor now transferred to Induratia are known producers of bioactive volatile organic compounds (VOCs) with many industrial applications. However, the members of this genus have rarely been reported to produce non-volatile metabolites including enzyme. Enzymes of the endophytes are degraders of the polysaccharides available in the host plants and the knowledge of enzyme production by Induratia spp. may provide insights into their possible biotechnological applications. The aim of this study was to evaluate the activity of amylase, cellulase, lipase, pectinase, phytase, protease, endo β-1,4 glucanase and exo β-1,4 glucanase enzymes produced by fungi of the species Induratia coffeana, Induratia yucatanensis and Induratia sp. isolated from organic coffee plants. All Induratia spp. were able to produce the extracellular enzymes cellulase, pectinase, protease, and phytase. Eight fungi were able to produce lipase and four produced amylase. The specific activity of endo β-1, 4 glucanase and exo β-1,4 glucanase enzymes were detected for 9 and 8 endophytic fungi, respectively. This work demonstrated for the first time, the array of enzymes produced by Induratia spp. isolated from Coffea arabica in organic systems in Brazil.
Phialomyces macrosporus reduces Cercospora coffeicola survival on symptomatic coffee leaves
(Editora UFLA, 2019-01) Laborde, Marie Caroline Ferreira; Botelho, Deila Magna dos Santos; Rodríguez, Gabriel Alfonso Alvarez; Resende, Mário Lúcio Vilela de; Queiroz, Marisa Vieira de; Batista, Aline Duarte; Cardoso, Patrícia Gomes; Pascholati, Sérgio Florentino; Gusmão, Luis Fernando Pascholati; Martins, Samuel Júlio; Medeiros, Flávio Henrique Vasconcelos de
Brown eye spot is among the most important coffee diseases, it is caused by a necrotrophic fungal Cercospora coffeicola. Saprobe fungi have potential in reducing the survival of necrotrophic pathogens and can act through competition of nutrients, mycoparasitism, antibiosis and resistance induction. We have screened saprobe fungi for the ability to reduce C. coffeicola sporulation and viability and determined the possible mechanisms involved in the biocontrol. The selected saprobe fungus, Phialomyces macrosporus, reduced the germination of C. coffeicola conidia by 40%. P. macrosporus produced both volatile and non-volatile compounds that inhibited C. coffeicola growth, sporulation and viability. The production of antimicrobial substances was the main mode of action used by the saprobe fungi. Therefore, P. macrosporus is a promising biological agent for the integrated management of brown eye spot
Prospecting of endophytic fungi producing volatile organic compounds: taxonomy, identification of volatiles and potential uses for the biological control of postharvest diseases
(Universidade Federal de Viçosa, 2017-02-17) Gomes, André Angelo Medeiros; Pereira, Olinto Liparini; Lopes, Ueder Pedro; Queiroz, Marisa Vieira de
In the search for alternative strategies to control of postharvest diseases in fruits and vegetables aimed at reducing the use of fungicides, was conducted a prospection to endophytic fungi capable of emitting volatile compounds with antimicrobial properties. Through parallel growth technique, directed towards the isolation of fungi producing volatile antimicrobials, endophytic isolates of coffee and carqueja plants were obtained and in preliminary test inhibited the growth of Aspergillus ochraceus by the emission of volatile compounds. The presence of at least three distinct species of Muscodor was verified, and a new taxon of Simplicillium by clarifying the identity of the endophytic isolates obtained through comparative studies of morphological structures, BLAST of gene sequences and phylogenetic analyzes. M. yucatanensis, Muscodor sp. and M. coffeanum isolates were obtained from coffee branches, the latter also isolated in leaves of carqueja. The antimicrobial activity of these isolates was demonstrated against Aspergillus species frequently associated with coffee beans by in vitro mycofumigation. In addition, mycofumigation with M. coffeanum CDA 741 inhibited the growth of A. ochraceus inoculated in coffee beans. The compounds present in the volatile mixture emitted by each isolate were identified by solid-phase micro-extraction gas chromatography and mass spectroscopy. Simplicillium sp. CDA 734, a new species of endophytic Cordycipitaceae from coffee plants, will be proposed through comparative studies of its morphology combined with phylogenetic analyzes. Simplicillium sp. CDA 734 inhibited the growth of A. ochraceus, A. tubingensis, A. sydowii and A. niger by mycofumigation. Among the compounds identified in the volatile mixture emitted by Simplicillium sp. CDA 734, 1-Propanone, 1-(5-methyl-2-furanyl)-, Cyclopropane, 1- ethoxy-2,2-dimethyl-3-(2-phenylethynyl)-, and 2-Propenoic acid, 3-(2-formyl-4- methoxyphenyl)-, ethyl ester, (E)- were those with the highest percentage of peak per area. In mycofumigation of organic strawberries inoculated with Colletotrichum acutatum e Botrytis cinerea, M. coffeanum CDA 739 decreases 100 and 81% the incidence of anthracnose and gray mold in the fruits, respectively. Mycofumigation through sachets containing rye grains colonized by M. coffeanum CDA 739 significantly reduced the incidence of anthracnose in fruits inoculated with C. acutatum, making possible its use in large-scale to control postharvest strawberry diseases through mycofumigation.