Navegando por Autor "Zapata-Zapata, Arley David"
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Item Functional diversity of microbial communities associated with fermentation processes in coffee (Coffea arabica L.)(Editora UFLA, 2021) Peñuela-Martínez, Aida Esther; Romero-Tabarez, Magally; Zapata-Zapata, Arley DavidMucilage fermentation conducted to degrade and remove coffee mucilage, is an important stage to also define coffee quality, but each microorganism’s contribution to the final quality is not yet known. Therefore, tools are needed to easily identify this relationship in order to be used to enhance coffee quality. The present manuscript describes the behavior of the microbial communities present in fermentations conducted under various conditions, which produced differences in the sensory quality of the coffee as assessed by the community-level catabolic profile approach. The coffee samples came from six different fermentation processes that produced coffee in two quality classifications according to the Specialty Coffee Association (SCA) protocol: very good and excellent. Functional diversity of the microbial communities and substrate consumption were compared through analysis of variance. The multidimensional scaling analysis was used to identify the similarities or differences between treatments. The indices of functional diversity revealed significant differences and direct proportionality with the quality rating. Diversity index (H) was between 2.09 and 2.71 and Evenness was between 1.75 and 2.21. The consumption of groups of substrates was different between fermentations, especially in carbohydrates and carboxylic acids, and the greatest consumption of these was found in the excellent-quality coffee. The different fermentative processes evaluated by this technique showed a high metabolic activity related to the great diversity of substrates given to the microbial communities and microorganisms involved, causing reactions that had influence on the final quality of the product.Item Performance of different fermentation methods and the effect on coffee quality (Coffea arabica L.)(Editora UFLA, 2018-10) Peñuela-Martínez, Aida Esther; Zapata-Zapata, Arley David; Durango-Restrepo, Diego LuisFermentation of coffee mucilage is a spontaneous process caused by microorganisms growing in the environment, which is influenced by factors such as the coffee variety, climate and fruit maturity. These external factors play an important role in fermentation evolution because they have effect on the microorganism activity and the time for substrate transformation. The aim of this research was to asses different fermentation wet process and evaluate their effect on coffee quality (C. arabica ), as well as on organic acid concentrations and volatile organic compounds content, in the green coffee beans. The study was divided in two phases, one in which the pulping time was delayed and the fermentation methods were modified, and the second phase in which a bioreactor was used to control the pH and temperature of the coffee mass during fermentation. Two control treatments were used: without fermentation (mechanical removal of mucilage) and the traditional fermentation done in the farm. Significant differences in coffee quality were observed. The best quality was obtained from the treatments that used short process times and low temperatures. The concentrations of acetic, lactic and citric acids between the treatments and the control treatments were different. Higher contents of esters and ketones were found in the coffee that obtained the highest quality. The assessed processes lead to the conclusions that it is possible to improve coffee quality throughout introducing changes in the fermentation process, as well as modulating the acidity and fragrance of the final product.