Genetics and Molecular Biology
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/13110
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Item Caffeine content of Ethiopian Coffea arabica beans(Sociedade Brasileira de Genética, 2000) Silvarolla, Maria Bernadete; Mazzafera, Paulo; Lima, Marinez Muraro Alves deThe coffee germplasm bank of the Instituto Agronômico de Campinas has many Coffea arabica accessions from Ethiopia, which is considered the primary center of genetic diversity in coffee plants. An evaluation of the caffeine content of beans from 99 progenies revealed intra- and inter-progeny variability. In 68 progenies from the Kaffa region we found caffeine values in the range 0.46-2.82% (mean 1.18%), and in 22 progenies from Illubabor region these values ranged from 0.42 to 2.90% (mean 1.10%). This variability could be exploited in a breeding program aimed at producing beans with low-caffeine content.Item Caffeine inheritance in interspecific hybrids of Coffea arabica x Coffea canephora (Gentianales, Rubiaceae)(Sociedade Brasileira de Genética, 2008) Priolli, Regina H.G.; Mazzafera, Paulo; Siqueira, Walter J.; Möller, Milene; Zucchi, Maria Imaculada; Ramos, Luis Carlos S.; Gallo, Paulo B.; Colombo, Carlos A.Caffeine inheritance was investigated in F2 and BC1F1 generations between Coffea arabica var. Bourbon Vermelho (BV) and Coffea canephora var. Robusta 4x (R4x). The caffeine content of seeds and leaves was determined during 2004 and 2005. Microsatellite loci-markers were used to deduce the meiotic pattern of chromosome pairing of tetraploid interspecific hybrids. Genetic analysis indicated that caffeine content in seeds was quantitatively inherited and controlled by genes with additive effects. The estimates of broad-sense heritability of caffeine content in seeds were high for both generations. In coffee leaves, the caffeine content (BSH) from the same populations showed transgressive segregants with enhanced levels and high BSH. Segregation of loci-markers in BC1F1 populations showed that the ratios of the gametes genotype did not differ significantly from those expected assuming random associations and tetrasomic inheritance. The results confirm the existence of distinct mechanisms controlling the caffeine content in seeds and leaves, the gene exchange between the C. arabica BV and C. canephora R4x genomes and favorable conditions for improving caffeine content in this coffee population.