Anais da Academia Brasileira de Ciências
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/13096
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Item Impact of coffee biochar on carbon, microbial biomass and enzyme activities of a sandy soil cultivated with bean(Academia Brasileira de Ciências, 2021) Martins Filho, Argemiro P.; Medeiros, Erika V. de; Lima, José Romualdo S.; Costa, Diogo P. da; Duda, Gustavo P.; Silva, Jenifer S. A. da; Oliveira, Julyana B. de; Antonino, Antônio C. D.; Menezes, Rômulo S. C.; Hammecker, ClaudeBiochar has been used to reuse the agro-industrial wastes and improve soil quality. Several studies have been carried out to show the impact of biochar on physical and chemical soil attributes. However, there are still gaps regarding the effects on as microbial biomass and enzymatic activities that are important to determine sensitive indicators to evaluate changes in management practices. The objective of this study was to assess the effect of two biochars on the chemical, microbial biomass carbon, and the enzymatic activities in an Entisol cultivated with bean. We evaluate two types of coffee biochar: ground and husks, four doses (4, 8, 12, and 16 Mg ha-1) and control. All treatments received organic fertilization with cow manure. Husks biochar increase the soil pH, Ca, and K, also contributing to the reduction of toxic aluminum contentes and raising the concentrations of P labile. The treatments that received ground biochar showed higher soil organic carbon, microbial biomass, β-glucosidase, and fl uorescein diacetate. Biochar produced from coffee residues increased sandy soil quality. We showed the fi rst report on the benefi cial impact of coffee biochar on enzymatic and microbiological quality of sandy soil cultivated with the bean.Item Purification and characterization of a protease from Aspergillus sydowii URM5774: Coffee ground residue for protease production by solid state fermentation(Academia Brasileira de Ciências, 2021) Rocha, Felype T. B.; Brandão-Costa, Romero M. P.; Neves, Anna Gabrielly D.; Cardoso, Kethylen B. B.; Nascimento, Thiago P.; Albuquerque, Wendell W. C.; Porto, Ana Lúcia F.Solid state fermentation is a promising technology largely used in biotechnology process and is a suitable strategy for producing low-cost enzymatic products. At the present study, a novel enzyme obtained through solid state fermentation using Aspergillus sydowii was herein purifi ed and characterized. The fermentations used coffee ground residue as substrate and the crude enzyme was submitted through further purifi cation steps of: acetonic precipitation, DEAE-Sephadex and Superdex G-75 column. Both crude and purifi ed enzymes were submitted to biochemical characterization of their thermostability, optimal temperature and pH, effects of inhibitors and metal ions. A purifi ed protease was obtained with yield of 5.9-fold and 53% recovery, with maximal proteolytic activity of 352.0 U/mL. SDS-PAGE revealed a band of protein at 47.0 kDa. The enzyme activity was abolished in the presence of phenyl-methyl sulfonyl fl uoride and partially inhibited against Triton X-100 (78.0%). The optimal activity was found in pH 8.0 at 45°C of temperature. Besides, the enzyme showed stability between 35°C and 50°C. It was possible to determine appropriate conditions to the obtainment of thermostable proteases with biotechnological interest associated with a method that concomitantly shows excellent production levels and recovery waste raw material in a very profi table process.