MANEJO DE MELOIDOGYNE PARANAENSIS EM MUDAS DE CAFEEIRO COM O FUNGO PAECILOMYCES LILACINUS
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2009
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O objetivo deste trabalho foi avaliar o isolado de Paecilomyces lilacinus mais eficiente e a sua melhor formulação (pó ou emulsão) para reduzir a população de Meloidogyne paranaensis em mudas de cafeeiro altamente infestadas. O experimento foi conduzido em casa-de-vegetação na Universidade Estadual de Londrina, no Centro de Ciências Agrárias. O delineamento experimental utilizado foi em blocos casualizados contendo nove tratamentos (Isolado Pae18 pó e emulsão; Isolado Pae 22 pó e emulsão; Nemat pó e emulsão; Nemaplus®; estemunha inoculada com M. paranaensis e testemunha sem a inoculação do nematóide) e cada tratamento com 10 replicas. Passado cinco dias da inoculação do nematóide foram aplicados 2 gramas ou ml de cada produto em questão por planta. E após 60 dias foram avaliados a altura das plantas; diâmetro de caule; pesos da parte aérea e sistema radicular e massas de ovos por sistema radicular, por meio da imersão em solução de floxina B 0,015%, durante 15 minutos, para coloração das mesmas; número de fêmeas que penetraram nas raízes, segundo a metodologia de Bird et al., 1983; extração dos ovos (Boneti & Ferraz, 1981). Os dados obtidos aos 60 dias após a aplicação dos tratamentos foram submetidos às análises de variância e ao teste de Tukey à 5% de probabilidade de erro. Os isolados de P. lilacinus não afetaram a altura e diâmetro do caule do cafeeiro. Os isolados de P. lilacinus não afetaram o peso fresco da parte aérea e de raízes do cafeeiro. A população de juvenis do segundo estádio (J2) de M. paranaensis no solo não foi reduzida pelos isolados testados na formulação Pó e Emulsão. Todos os isolados mostraram capacidade na redução do número de ovos nas raízes em comparação com a testemunha, especialmente o tratamento onde se utilizou todos os isolados em mistura na formulação em Emulsão, ao isolado Pae 18 na formulação Pó e ao isolado Pae 22 na formulação em Emulsão.
The objective of this study was to evaluate the isolation of Paecilomyces lilacinus more efficient and the best formulation (powder or emulsion) to reduce the population of Meloidogyne paranaensis in highly infested seedlings of coffee. The experiment was conducted green house in Londrina State University, Center for Agricultural Sciences. The experimental design was randomized blocks with nine treatments (Isolate Pae18 powder and emulsion, 22 Pae Isolate powder and emulsion, powder and emulsion Nemat; Nemaplus ®; witness inoculated with M. paranaensis and control without the inoculation of the nematode) and each treatment with 10 replicas. After five days of inoculation of the nematode were applied 2 g or ml of product in question by each plant. And after 60 days were the height of plants, stem diameter, weight of shoot and root masses and of eggs per root system, through immersion in a solution of floxina B 0015%, 15 minutes for staining the same , number of females that entered the roots, according to the method of Bird et al., 1983; extraction of eggs (Boneti & Ferraz, 1981). Data obtained at 60 days after application of the treatments were submitted to variance analysis and Tukey test to 5% error probability. Isolates of P. lilacinus did not affect the height and diameter of the stem of the coffee. Isolates of P. lilacinus did not affect the fresh weight of shoots and roots of coffee. The population of the second stage juveniles (J2) of M. paranaensis the soil was not reduced by the isolates tested in powder and emulsion formulation. All isolates showed ability to reduce the number of eggs in the roots compared to control, especially where the treatment was used in all isolates in the formulation mixture in emulsion, 18 Pae to isolate the powder formulation and isolated in the formulation Pae 22 emulsion.
The objective of this study was to evaluate the isolation of Paecilomyces lilacinus more efficient and the best formulation (powder or emulsion) to reduce the population of Meloidogyne paranaensis in highly infested seedlings of coffee. The experiment was conducted green house in Londrina State University, Center for Agricultural Sciences. The experimental design was randomized blocks with nine treatments (Isolate Pae18 powder and emulsion, 22 Pae Isolate powder and emulsion, powder and emulsion Nemat; Nemaplus ®; witness inoculated with M. paranaensis and control without the inoculation of the nematode) and each treatment with 10 replicas. After five days of inoculation of the nematode were applied 2 g or ml of product in question by each plant. And after 60 days were the height of plants, stem diameter, weight of shoot and root masses and of eggs per root system, through immersion in a solution of floxina B 0015%, 15 minutes for staining the same , number of females that entered the roots, according to the method of Bird et al., 1983; extraction of eggs (Boneti & Ferraz, 1981). Data obtained at 60 days after application of the treatments were submitted to variance analysis and Tukey test to 5% error probability. Isolates of P. lilacinus did not affect the height and diameter of the stem of the coffee. Isolates of P. lilacinus did not affect the fresh weight of shoots and roots of coffee. The population of the second stage juveniles (J2) of M. paranaensis the soil was not reduced by the isolates tested in powder and emulsion formulation. All isolates showed ability to reduce the number of eggs in the roots compared to control, especially where the treatment was used in all isolates in the formulation mixture in emulsion, 18 Pae to isolate the powder formulation and isolated in the formulation Pae 22 emulsion.
Descrição
Trabalho apresentado no Simpósio de Pesquisa dos Cafés do Brasil (6. : 2009 : Vitória, ES). Anais Brasília, D.F: Embrapa - Café, 2011
Palavras-chave
P. lilacinus, formulação, café, M. paranaensis., P. lilacinus, formulation, coffee, M. paranaensis.
Citação
Baida, Fernando César; Santiago, Débora Cristina; Cadioli, Marina Capparelli; Stroze, Camila Torres; Foganholi, Ana Paula do Amaral Mônaco. Manejo de Meloidogyne paranaensis em mudas de cafeeiro com fungo Paecilomyces lilacinus. In: Simpósio de Pesquisa dos cafés do Brasil (6. : 2009 : Vitória, ES). Anais Brasília, D.F: Embrapa - Café, 2011 (1 CD-ROM), 4p.