Óleo essencial e extrato de cravo-da-índia no controle de Colletotrichum gloeosporioides, agente da mancha manteigosa, em sementes e mudas de café
Data
2009-02-19
Autores
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Editor
Universidade Federal de Lavras
Resumo
O presente trabalho teve como objetivo avaliar o efeito do óleo essencial de cravo-da-índia (Sizygium aromaticum) a 0,25%, 0,5%, 0,75% e 1,0% e extrato de cravo-da-índia a 0,1%, 1,0%, 10% e 20%): (i) na inibição do crescimento micelial de C. gloeosporioides; (ii) na incidência de Colletotrichum gloeosporioides e de outros fungos associados às sementes; (iii) em parâmetros fisiológicos de sementes e plântulas de café; (iv) no controle da mancha manteigosa em mudas de cafeeiro; (v) e na produção de matéria seca (MS) da parte aérea e da raiz das mudas. O óleo essencial e o extrato de cravo-da-índia apresentaram-se como produtos potenciais, inibindo o crescimento micelial de C. gloeosporioides, in vitro. Nas sementes, foi constatada uma microflora formada por C. gloeosporioides e fungos dos gêneros Aspergillus, Fusarium e Cladosporium, sendo que o óleo e extrato reduziram a ocorrência de alguns desses fungos. O óleo de cravo a 0,5% e 1,0% e o extrato de cravo a 20% promoveram um aumento da porcentagem de sementes normais de café. O óleo de cravo a 0,25%, 0,5% e 1,0% e o extrato de cravo a 20% promoveram aumento do IVE das sementes. Todos os tratamentos com óleos e extratos com exceção do óleo a 0,75% e extrato a 1,0% promoveram uma maior produção de MS da parte aérea das plântulas de café. O extrato a 0,1%, 10% e 20% e o óleo essencial de cravo a 0,5%, 0,75% e 1,0% apresentaram-se como produtos potenciais na redução da AACPS da doença. O óleo de cravo a 0,75%, o extrato de cravo nas concentrações 0,1%, 1,0% e 10%, e o fungicida apresentaram maior MS de parte aérea para as mudas doentes. O extrato de cravo a 10% apresentou maior MS de parte aérea para as mudas doentes do que para as mudas sadias. O fungicida e a testemunha inoculada apresentaram maior MS de raiz para as mudas sadias. A testemunha inoculada apresentou maior MS de raiz para as mudas sadias do que para as mudas doentes.
This study aimed to evaluate the effect of essential oil of clove (Sizygium aromaticum) at 0.25%, 0.5%, 0.75%, 1.0%, and extract at 0.1%, 1.0%, 10% and 20% in: (i) the inhibition of mycelial growth of C. gloeosporioides, in vitro, (ii) the incidence of C. gloeosporioides and other fungi associated with seeds, (iii) the physiologic parameter from seeds and seedlings of coffee; (iv) in the control of the battery spot; (v) and in the production of dry mass (DM) from roots and foliage of seedlings. The essential oil and extract of clove showed it were potential products to inhibiting the mycelial growth of C. gloeosporioides, in vitro. It was found a microflora formed by C. gloeosporioides and fungi of the genus Aspergillus, Fusarium and Cladosporium. The clove essential oil and extract reduced the occurrence of some these fungi. The essential oil at 0,5% e 1,0% and extract at 20% stimulated the germination of coffee seeds. The clove oil at concentrations 0.25%, 0.5% and 1,0% and the extract at 20% showed higher IVG. All treatments with the oil and extract, with the exception of oil at 0.75% and fungicide, promoted greater production of DM of the foliage of the seedlings in relation to control. For disease seedlings, clove extract at 0.1%, 10% and 20%, and clove oil at 0.5%, 0.75% and 1.0% had the lowest area under the disease progress curve (AUDPC). The clove oil at 0.75%, the extract of clove in the concentrations 0.1%, 1.0% and 10%, and the Chlorothalonil fungicide had higher foliage DM in the disease seedlings. The extract of clove at 10% showed greater foliage DM for the diseased seedlings than healthy seedlings. The fungicide and inoculated control had higher root DM than healthy seedlings. The inoculated control had higher root DM for healthy seedlings than for diseased seedlings.
This study aimed to evaluate the effect of essential oil of clove (Sizygium aromaticum) at 0.25%, 0.5%, 0.75%, 1.0%, and extract at 0.1%, 1.0%, 10% and 20% in: (i) the inhibition of mycelial growth of C. gloeosporioides, in vitro, (ii) the incidence of C. gloeosporioides and other fungi associated with seeds, (iii) the physiologic parameter from seeds and seedlings of coffee; (iv) in the control of the battery spot; (v) and in the production of dry mass (DM) from roots and foliage of seedlings. The essential oil and extract of clove showed it were potential products to inhibiting the mycelial growth of C. gloeosporioides, in vitro. It was found a microflora formed by C. gloeosporioides and fungi of the genus Aspergillus, Fusarium and Cladosporium. The clove essential oil and extract reduced the occurrence of some these fungi. The essential oil at 0,5% e 1,0% and extract at 20% stimulated the germination of coffee seeds. The clove oil at concentrations 0.25%, 0.5% and 1,0% and the extract at 20% showed higher IVG. All treatments with the oil and extract, with the exception of oil at 0.75% and fungicide, promoted greater production of DM of the foliage of the seedlings in relation to control. For disease seedlings, clove extract at 0.1%, 10% and 20%, and clove oil at 0.5%, 0.75% and 1.0% had the lowest area under the disease progress curve (AUDPC). The clove oil at 0.75%, the extract of clove in the concentrations 0.1%, 1.0% and 10%, and the Chlorothalonil fungicide had higher foliage DM in the disease seedlings. The extract of clove at 10% showed greater foliage DM for the diseased seedlings than healthy seedlings. The fungicide and inoculated control had higher root DM than healthy seedlings. The inoculated control had higher root DM for healthy seedlings than for diseased seedlings.
Descrição
Dissertação de Mestrado defendida na Universidade Federal de Lavras
Palavras-chave
Coffea arabica, Controle alternativo, Colletotrichum gloeosporioides, Óleos essenciais, Extratos vegetais
Citação
PIERRE, R. O. Óleo essencial e extrato de cravo-da-índia no controle de Colletotrichum gloeosporioides, agente da mancha manteigosa, em sementes e mudas de café. 2009. 61 f. Dissertação (Mestrado em Agronomia/Fitopatologia) - Universidade Federal de Lavras, Lavras. 2009.