Uso de marcadores RAPD no estudo de variabilidade em clones de Coffea canephora var. Conillon
Arquivos
Data
2000
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Avaliou-se quatro repetições de 12 clones que apresentaram variabilidade de reação à H. vastatrix. Oito indivíduos dos clones 03 e 07 foram analisados quando inoculados com as raças I, II, III e XIII. Selecionou-se também, cinco clones que apresentaram reação uniforme quando inoculados com quatro raças do patógeno, totalizando 76 indivíduos. Pelo método de agrupamento de Tocher, os 76 indivíduos formaram 16 grupos. As quatro repetições de cada clone formaram grupos distintos, entretanto, o indivíduo 21, correspondente à repetição I do clone 104-A, formou isoladamente o grupo 16, mostrando-se divergente dos demais. Os clones 104-A e 104-B formaram um grupo, e os clones 110-A e 110-B, outro grupo, indicando que são muito próximos geneticamente. Os primers utilizados não foram capazes de detectar diferenças entre os clones quando se considera o ciclo de maturação, da mesma forma que não foi possível observar diferenças intraclones. Por meio de marcadores RAPD, não foi possível detectar diferenças intraclones da variedade Conillon que apresentaram instabilidade de reações a H. vastatrix. Da mesma forma, não foi possível diferenciar clones de diferentes ciclos de maturação. A técnica de RAPD foi eficaz
no estudo de variabilidade genética em C. canephora.
Four replication of 12 clones that showed reaction variability for H. vastatrix were evaluated. Eight plants of the clones 03 and 07 were analyzed when inoculated with the races I, II, III and XIII. It was also selected five clones that showed uniform reaction when inoculated with four races of the pathogen, giving a total of 76 plants. By the Tocher method of agroupment, 16 groups were formed. The four replications of each clone formed distinct groups; however, the plant clone 21 that belongs to the replication I of the clone 104-A, formed one isolated group (16), showing that it was different from the others. The clones 104-A and 104-B formed one group, and the clones 110-A and 110-B another, indicating that they are genetically very close. The primers utilized were not able to detect differences between the clones when we analyzed the maturation cycle. Also, it was not possible to detect intraclones differences. By the use of RAPD markers, it was not possible to detect intraclones differences of the variety Conillon that showed instability of reactions to H. vastatrix. In the same way, it was not possible to differentiate clones of different maturation cycles. The RAPD techniques was efficient to study genetic variability in C. canephora.
Four replication of 12 clones that showed reaction variability for H. vastatrix were evaluated. Eight plants of the clones 03 and 07 were analyzed when inoculated with the races I, II, III and XIII. It was also selected five clones that showed uniform reaction when inoculated with four races of the pathogen, giving a total of 76 plants. By the Tocher method of agroupment, 16 groups were formed. The four replications of each clone formed distinct groups; however, the plant clone 21 that belongs to the replication I of the clone 104-A, formed one isolated group (16), showing that it was different from the others. The clones 104-A and 104-B formed one group, and the clones 110-A and 110-B another, indicating that they are genetically very close. The primers utilized were not able to detect differences between the clones when we analyzed the maturation cycle. Also, it was not possible to detect intraclones differences. By the use of RAPD markers, it was not possible to detect intraclones differences of the variety Conillon that showed instability of reactions to H. vastatrix. In the same way, it was not possible to differentiate clones of different maturation cycles. The RAPD techniques was efficient to study genetic variability in C. canephora.
Descrição
Trabalho apresentado no Simpósio de Pesquisa dos Cafés do Brasil (1.: 2000 : Poços de Caldas, MG). Resumos expandidos. Brasília, D.F. : Embrapa Café; Belo Horizonte : Minasplan, 2000. 2v. (1490p.) : il.
Palavras-chave
RAPD Clones, Coffea canephora Conillon Variabilidade genética, RAPD Clones Coffea canephora Conillon Genetic variability
Citação
Silva, Dalza G., Zambolim, Laércio, Sakiyama, Ney S., Sakiyama, Cássia C. H. , Fonseca, Aymbiré F. A., Pereira, Antonio A., Teixeira, Terezinha A. Uso de marcadores RAPD no estudo de variabilidade em clones de Coffea canephora var. Conillon. In: Simpósio de Pesquisa dos Cafés do Brasil (1.: 2000 : Poços de Caldas, MG). Resumos expandidos. Brasília, D.F. : Embrapa Café; Belo Horizonte : Minasplan, 2000. 2v. (1490p.), p. 134-137.