Caracterização de populações de Meloidogyne exigua associadas a cafeeiros na Zona da Mata de Minas Gerais
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Data
2002
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Universidade Federal de Viçosa
Resumo
Para determinar a ocorrência e variabilidade de Meloidogyne spp. em cafeeiros da região da Zona da Mata de Minas Gerais, 57 populações de 16 municípios foram avaliadas pela caracterização morfológica e enzimática e pela gama de hospedeiros. Todas as populações foram identificadas como M. exigua por meio das configurações perineais, mas as populações de São João do Manhuaçu mostraram perineal similar àquela de M. arenaria. Contudo, a identificação de todas as populações como M. exigua foi confirmada por fenótipos de esterase, malato desidrogenase, superóxido dismutase e glutamato oxaloacetato transminase. Treze populações apresentaram o fenótipo de esterase típico de M. exigua (VF1), enquanto a maioria das populações (77,2%) exibiu o fenótipo de duas bandas (VF2). Nenhuma variabilidade fisiológica intra-específica foi observada nas populações estudadas, e todas elas foram capazes de se reproduzir em plantas de tomate, pimentão, cacau, cebola, feijão e soja. A reprodução dessas populações em plantas de tomate e pimentão foi maior do que em mudas de cafeeiro, utilizadas como padrão de suscetibilidade.
Minas Gerais State is the most important producer of coffee (Coffea arabica) in Brazil and 28% of its production occurs in the Zona da Mata region. Four major species of root-knot nematodes attacking coffee plants have been reported in Brazil, and some of them can cause plant death. The correct identification of species and, or race (s) of Meloidogyne present in roots of coffee is extremely important in deciding which measures are more appropriate for controlling the pathogens. In order to determine the occurrence and variability of Meloidogyne spp. in the region, 57 populations from 16 locations were evaluated based on morphologic, enzymatic and physiologic traits. All the 57 populations were identified as Meloidogyne exigua based on their perineal patterns, but the populations from São João do Manhuaçu showed perineal pattern very similar to M. arenaria. Even though, the identification of all populations was confirmed by phenotypes of esterase, malate dehydrogenase, sulfoxide dismutase and glutamate oxalo-acetate transaminase. Thirteen populations presented the typical esterase phenotype showed by one band (VF1), while most of the populations (77,2%) exhibit a phenotype showed by two bands (VF2). No intraspecific physiological variability was observed in the studied populations, and all of them were able to reproduce on tomato (Lycopersicon esculentum), pepper (Capsicum annuum), cocoa (Theobroma cacao), onion (Allium cepa), bean (Phaseolus vulgaris) and soybean (Glycine max). The reproduction of those populations on tomato and on pepper plants was higher than on coffee seedlings (standard of susceptibility).
Minas Gerais State is the most important producer of coffee (Coffea arabica) in Brazil and 28% of its production occurs in the Zona da Mata region. Four major species of root-knot nematodes attacking coffee plants have been reported in Brazil, and some of them can cause plant death. The correct identification of species and, or race (s) of Meloidogyne present in roots of coffee is extremely important in deciding which measures are more appropriate for controlling the pathogens. In order to determine the occurrence and variability of Meloidogyne spp. in the region, 57 populations from 16 locations were evaluated based on morphologic, enzymatic and physiologic traits. All the 57 populations were identified as Meloidogyne exigua based on their perineal patterns, but the populations from São João do Manhuaçu showed perineal pattern very similar to M. arenaria. Even though, the identification of all populations was confirmed by phenotypes of esterase, malate dehydrogenase, sulfoxide dismutase and glutamate oxalo-acetate transaminase. Thirteen populations presented the typical esterase phenotype showed by one band (VF1), while most of the populations (77,2%) exhibit a phenotype showed by two bands (VF2). No intraspecific physiological variability was observed in the studied populations, and all of them were able to reproduce on tomato (Lycopersicon esculentum), pepper (Capsicum annuum), cocoa (Theobroma cacao), onion (Allium cepa), bean (Phaseolus vulgaris) and soybean (Glycine max). The reproduction of those populations on tomato and on pepper plants was higher than on coffee seedlings (standard of susceptibility).
Descrição
Dissertação de Mestrado defendida na Universidade Federal de Viçosa
Palavras-chave
Café Nematóides fitopatogênicos Meloidogyne exigua Caracterização Morfologia Plantas hospedeiras Eletroforose de iso-enzimas, Coffee Plant pathogenis nematodes Meloidogyne exigua Characterization Morphology Host plants
Citação
Oliveira, Dagoberto Saunders de. Caracterização de populações de Meloidogyne exigua associadas a cafeeiros na Zona da Mata de Minas Gerais. Viçosa : UFV, 2002. 48p. : il. (Dissertação - mestrado em Fitopatologia) Orientadora: Rosângela D’Arc de Lima Oliveira T 595.18204524 o48c 2002