Açúcares solúveis, sacarose sintase e sacarose fosfato sintase durante o desenvolvimento do fruto de café, sob diferentes condições de luz e carga
Arquivos
Data
2007
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Resumo
Foram abordados nesse trabalho aspectos fisiológicos de carboidratos envolvidos na relação fonte-dreno, sendo a sacarose o principal carboidrato exportado. Sabendo-se que a sacarose não é utilizada diretamente como substrato para a maioria dos processos envolvidos no crescimento, desenvolvimento e armazenamento, tanto na fonte como no dreno, o destino metabólico da sacarose é mediado pelas enzimas invertases, sacarose sintase e sacarose fosfato sintase. Nesse estudo foram dosadas as enzimas sintase da sacarose (SUSY) e sacarose fosfato sintase (SPS), assim como os teores de açúcares solúveis totais, redutores e sacarose, durante o desenvolvimento do fruto de cafeeiro em diferentes tecidos: polpa, perisperma e endosperma e em diferentes condições de tratamento: controle, onde as plantas foram expostas a pleno sol; com sombrite 50% e com carga do cafeeiro reduzida à 30%. Foi mostrado que, apesar de SUSY e SPS tenderem a ter menor atividade nos tratamentos de sol e menor produção, os teores de açúcares não variaram. Foi observado que as enzimas seguem o mesmo padrão de atividade em todos os tecidos aumentando com a maturação, independente do tratamento.
The physiological aspects of carbohydrate metabolism and the sink-source relationship were studied. Sucrose was the main sugar transported to fruits in coffee. Sucrose is not promptly used as substrate in most of the process related with growth, development and storage in source or sink tissues. It is first metabolized by invertases, sucrose synthase (SUSY) and sucrose phosphate synthase (SPS). The activities of SUSY and SPS as well as the contents of reducing sugars, sucrose and total soluble sugars were determined in the pulp, perisperm and endosperm during the development of coffee fruits, from plants under full sunlight (control), 50% light and carrying 30% of the fruits. Fruits were manually removed from the later treatment and the 50% light intensity was provided by a plastic screen (50% light cut-off). It was shown that although SUSY and SPS have lower activity in the treatments of less light and less fruits, the contents of sugars did not vary accordingly. It was observed the same pattern for enzyme activities in the tissues in the three treatments, an increase with maturation.
The physiological aspects of carbohydrate metabolism and the sink-source relationship were studied. Sucrose was the main sugar transported to fruits in coffee. Sucrose is not promptly used as substrate in most of the process related with growth, development and storage in source or sink tissues. It is first metabolized by invertases, sucrose synthase (SUSY) and sucrose phosphate synthase (SPS). The activities of SUSY and SPS as well as the contents of reducing sugars, sucrose and total soluble sugars were determined in the pulp, perisperm and endosperm during the development of coffee fruits, from plants under full sunlight (control), 50% light and carrying 30% of the fruits. Fruits were manually removed from the later treatment and the 50% light intensity was provided by a plastic screen (50% light cut-off). It was shown that although SUSY and SPS have lower activity in the treatments of less light and less fruits, the contents of sugars did not vary accordingly. It was observed the same pattern for enzyme activities in the tissues in the three treatments, an increase with maturation.
Descrição
Trabalho apresentado no Simpósio de Pesquisa dos Cafés do Brasil (5. : 2007 : Águas de Lindóia, SP). Anais. Brasília, D.F. : Embrapa Café, 2007.
Palavras-chave
Café Sacarose sintase Sacarose fosfato sintase, Coffee Sucrose synthase Sucrose phosphate synthase
Citação
Geromel, Clara; Mazzafera, Paulo; Marraccini, Pierre R.; Ferreira, Lucia P.; Vieira, Luiz G.E.; Pereira, Luiz F.P. Açúcares solúveis, sacarose sintase e sacarose fosfato sintase durante o desenvolvimento do fruto de café, sob diferentes condições de luz e carga. In: Simpósio de Pesquisa dos Cafés do Brasil (5. : Águas de Lindóia, SP : 2007). Anais. Brasília, D.F. : Embrapa - Café, 2007. (1 CD-ROM), 3p.