Controle da mancha manteigosa com fungos sapróbios em cafeeiro
Data
2013-05-13
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Lavras
Resumo
O presente trabalho foi realizado com o objetivo de avaliar os efeitos de fungos sapróbios no controle de Colletotrichum gloeosporioides. Foram realizados quatro experimentos. Mudas de cafeeiro foram tratadas com fungos sapróbios e sete dias após o tratamento foram inoculadas com Colletotrichum gloeosporioides. A seguir foram realizados testes in vitro para compreender os mecanismos envolvidos na defesa. Os fungos Memnoniella echinata, Chloridium virescens var chlamydosporium e Phialomyces macrosporus apresentaram redução de 49, 51 e 59%, respectivamente na área abaixo da curva de progresso da doença, obtida com base nos índices de severidade, de acordo com Shaner & Finney (1977). O fungo Phialomyces macrosporus foi testado in vitro em três experimentos. Em cultura pareada com Colletotrichum gloeosporioides, reduziu o diâmetro final da colônia do patógeno em três meios; em MEA, a redução foi de 75%. No teste de Cruz de Malta, Phialomyces macrosporus mostrou fraco poder de inibição a Colletotrichum gloeosporioides. No teste de compostos voláteis foram utilizados três combinações de meio, em placas de poliestireno, divididas ao meio, utilizando CMA para cultivo do fungo sapróbio e MEA para cultivo do patógeno. Observou-se redução de 57% no IVCM e 42,5% na esporulação do patógeno em comparação com a testemunha. Este trabalho mostrou que o fungo Phialomyces macrosporus é promissor no controle de Colletotrichum gloeosporioides em cafeeiro, mas ainda se fazem necessários estudos em campo, para que possa ser utilizado.
This present study was aimed to evaluate the effects of saprobe fungi in control of Colletotrichum gloeosporioides. Four experiments were carried out. Coffee seedlings were treated with saprobe fungi and seven days after treatment were inoculated with Colletotrichum gloeosporioides. Hereafter, in vitro tests were performed to understand the mechanisms involved in the defense. The Fungi Memnoniella echinata, Chloridium virescens var chlamydosporium and Phialomyces macrosporus decreased by 49, 51 and 59%, respectively in area under the disease progress curve, obtained based on disease severity according to Shaner & Finney (1977). The fungus Phialomyces macrosporus was tested in vitro in three experiments. With Phialomyces macrosporus three tests were performed in vitro. In paired culture with Colletotrichum gloeosporioides, the final diameter of the colony of the pathogen was reduced in three culture media, the reduction was 75% in MEA. In the test Cross Malta, Phialomyces macrosporus showed weak inhibition power to Colletotrichum gloeosporioides. In the test of volatile compounds were used three combinations of medium in polystyrene plates, divided in half, using CMA for cultivation of saprobe and MEA for growing the pathogen, it was reduced by 57% in ISMG and 42.5% in pathogen sporulation compared with the control. This research showed that the fungus Phialomyces macrosporus is promising for the control of Colletotrichum gloeosporioides in coffee, but further studies are needed in the field, so it can be used.
This present study was aimed to evaluate the effects of saprobe fungi in control of Colletotrichum gloeosporioides. Four experiments were carried out. Coffee seedlings were treated with saprobe fungi and seven days after treatment were inoculated with Colletotrichum gloeosporioides. Hereafter, in vitro tests were performed to understand the mechanisms involved in the defense. The Fungi Memnoniella echinata, Chloridium virescens var chlamydosporium and Phialomyces macrosporus decreased by 49, 51 and 59%, respectively in area under the disease progress curve, obtained based on disease severity according to Shaner & Finney (1977). The fungus Phialomyces macrosporus was tested in vitro in three experiments. With Phialomyces macrosporus three tests were performed in vitro. In paired culture with Colletotrichum gloeosporioides, the final diameter of the colony of the pathogen was reduced in three culture media, the reduction was 75% in MEA. In the test Cross Malta, Phialomyces macrosporus showed weak inhibition power to Colletotrichum gloeosporioides. In the test of volatile compounds were used three combinations of medium in polystyrene plates, divided in half, using CMA for cultivation of saprobe and MEA for growing the pathogen, it was reduced by 57% in ISMG and 42.5% in pathogen sporulation compared with the control. This research showed that the fungus Phialomyces macrosporus is promising for the control of Colletotrichum gloeosporioides in coffee, but further studies are needed in the field, so it can be used.
Descrição
Dissertação de Mestrado defendida na Universidade Federal de Lavras
Palavras-chave
Controle biológico, Colletotrichum gloeosporioides, Coffea arabica, Triagem, Phialomyces macrosporus
Citação
PINTO, F. A. M. F. Controle da mancha manteigosa com fungos sapróbios em cafeeiro. 2013. 72 f. Dissertação (Mestrado em Agronomia/Fitopatologia) - Universidade Federal de Lavras, Lavras. 2013.