Metabolismo do nitrogênio e estado nutricional do cafeeiro (Coffea arabica)
Data
2007
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Escola Superior de Agricultura “Luiz de Queiroz” - Universidade de São Paulo
Resumo
Este trabalho foi realizado com a finalidade de avaliar atividade da redutase do nitrato (RN), sintetase da glutamina (GS) e conteúdo de proteína total solúvel (PTS) em folhas de cafeeiro durante o desenvolvimento dos frutos em condições de campo. O experimento foi instalado em blocos ao acaso em esquema fatorial 3x6, constituído pela combinação de 3 doses de N (0, 150 e 350 kg ha -1 ) e avaliado em seis diferentes fases fenológicas (janeiro, fevereiro, março, abril, maio e junho), com sete repetições. No experimento foi utilizada a cultivar Catuaí Vermelho IAC 44 com seis anos implantada num Nitossolo Vermelho Eutroférrico, no município de Piracicaba-SP. Com relação à atividade da RN, GS e PTS em função das doses de N verificou incremento nos valores da atividade destas enzimas e do teor de proteína total solúvel em função da dose de N. As maiores atividades da RN foram obtidas no mês de janeiro (0,834, 1,370 e 1,781 μmol NO 2- g -1 MF h -1 ), bem como para a GS (82,47, 95,31 e 136,72 μmol У GH h -1 mg -1 PTS). O teor de proteína solúvel total (PTS) apresentou a mesma tendência (0,62, 5,66 e 6,52 mg mL -1 ). Estes valores foram obtidos em frutos na fase chumbinho. Durante o desenvolvimento dos frutos do cafeeiro a atividade das enzimas estudadas e o conteúdo de PTS foliar diminuíram, ao longo do tempo de janeiro a junho (maturação frutos).
The aim of this work was to evaluate the nitrate reductase (NR) and glutamine synthetase (GS) activity, total soluble protein content (PTS) in coffee leaves during the fruits development in field conditions. The study was carried out in ESALQ/USP (University of São Paulo), Piracicaba, São Paulo, Brazil in a Red Nitosol eutrofic. The experimental design was a complete randomized, in a factorial outline 3 x 6, constituted by combination of 3 levels (0, 150 and 350 kg ha -1 ) of nitrogen in six different periods (January, February, March, April, May and June) in plants (Cultivar Catuaí Vermelho IAC 44). The RN, GS activity and leaves PTS content was linear increased in relation of levels of N applied. In accordance with the present study, the enzymes had presented greater activity during the January than other months (0,834, 1,370 and 1,781 μmol NO 2- g -1 DF h -1 ) of RN; (82,47, 95,31 and 136,72 μmol -1 -1 -1 У GH h mg PTS) of GS and (0,62, 5,66 and 6,52 mg mL ) of total soluble protein (PTS), in the treatments T 0 , T 1 and T 2 , respectively. In January, the fruits were young grains and this period the coffee plant has high enzymatic activity. However, the capacity of nitrate assimilation during the annual cycle of coffee plants can be change through the environment and climatic variations and this phase (January) was verified a great precipitation. During the coffee fruits development, the RN and GS activity and the foliar PTS content had decreased, this occur due to the process of senescence of leaves, promote it the remobilization to other plant organs, as seed filling.
The aim of this work was to evaluate the nitrate reductase (NR) and glutamine synthetase (GS) activity, total soluble protein content (PTS) in coffee leaves during the fruits development in field conditions. The study was carried out in ESALQ/USP (University of São Paulo), Piracicaba, São Paulo, Brazil in a Red Nitosol eutrofic. The experimental design was a complete randomized, in a factorial outline 3 x 6, constituted by combination of 3 levels (0, 150 and 350 kg ha -1 ) of nitrogen in six different periods (January, February, March, April, May and June) in plants (Cultivar Catuaí Vermelho IAC 44). The RN, GS activity and leaves PTS content was linear increased in relation of levels of N applied. In accordance with the present study, the enzymes had presented greater activity during the January than other months (0,834, 1,370 and 1,781 μmol NO 2- g -1 DF h -1 ) of RN; (82,47, 95,31 and 136,72 μmol -1 -1 -1 У GH h mg PTS) of GS and (0,62, 5,66 and 6,52 mg mL ) of total soluble protein (PTS), in the treatments T 0 , T 1 and T 2 , respectively. In January, the fruits were young grains and this period the coffee plant has high enzymatic activity. However, the capacity of nitrate assimilation during the annual cycle of coffee plants can be change through the environment and climatic variations and this phase (January) was verified a great precipitation. During the coffee fruits development, the RN and GS activity and the foliar PTS content had decreased, this occur due to the process of senescence of leaves, promote it the remobilization to other plant organs, as seed filling.
Descrição
Dissertação de mestrado defendida na Escola Superior de Agricultura “Luiz de Queiroz” - Universidade de São Paulo
Palavras-chave
Aminoácidos, Ativação enzimática, Glutamina, Metabolismo mineral, Nitrogênio
Citação
REIS, A. R. Metabolismo do nitrogênio e estado nutricional do cafeeiro (Coffea arabica). 2007. 79 f. Dissertação (Mestrado em Fitotecnia) - Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba. 2007.