Análise in silico de aquaporinas potencialmente envolvidas com o estresse hídrico nas interações café- e citros-Xylella fastidiosa
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2007
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Resumo
No Brasil, um dos importantes problemas enfrentados por produtores de café e citros envolve os danos causados pela bactéria Xylella fastidiosa. Esta bactéria causa o entupimento dos vasos do xilema e impede a passagem de água e nutrientes, o que causa uma condição biológica de estresse hídrico. Os genomas de citros e café têm sido investigados funcionalmente e várias seqüências expressas (ESTs) foram identificadas a partir de diversas condições biológicas. Bibliotecas de EST utilizando folhas e ramos infectados com X. fastidiosa foram construídas em citros e café, respectivamente. A análise in silico das ESTs nesses tecidos revelou genes comuns expressos em ambas as culturas. Alguns destes genes codificam para proteínas relacionados com a resposta de estresse hídrico, incluindo a aquaporina, a "drought-induced protein Di19-like protein DIP" e a "fiber protein Fb2". Estes genes foram analisados neste estudo.
Currently, one of the important problems faced in Brazil by coffee and citrus producers involves the losses caused by the phytopathogenic bacterium Xylella fastidiosa. This bacterium colonizes xylem vessels probably resulting in reduced hydraulic conductance and thus, causing typical symptoms of water stress and deficiency in mineral nutrition. Functional genomic analyses have been performed in coffee and citrus and several ESTs were obtained from different biological conditions. The EST libraries from leaves and stems infected with X. fastidiosa were constructed in citrus and coffee, respectively. The in silico analysis of the expressed sequences in these tissues revealed genes commonly expressed in both cultures. Some of these genes are involved in water stress, including aquaporin, drought-induced protein Di19-like protein (DIP) and fiber protein Fb2. These genes were analyzed in this study.
Currently, one of the important problems faced in Brazil by coffee and citrus producers involves the losses caused by the phytopathogenic bacterium Xylella fastidiosa. This bacterium colonizes xylem vessels probably resulting in reduced hydraulic conductance and thus, causing typical symptoms of water stress and deficiency in mineral nutrition. Functional genomic analyses have been performed in coffee and citrus and several ESTs were obtained from different biological conditions. The EST libraries from leaves and stems infected with X. fastidiosa were constructed in citrus and coffee, respectively. The in silico analysis of the expressed sequences in these tissues revealed genes commonly expressed in both cultures. Some of these genes are involved in water stress, including aquaporin, drought-induced protein Di19-like protein (DIP) and fiber protein Fb2. These genes were analyzed in this study.
Descrição
Trabalho apresentado no Simpósio de Pesquisa dos Cafés do Brasil (5. : 2007 : Águas de Lindóia, SP). Anais. Brasília, D.F. : Embrapa Café, 2007.
Palavras-chave
EST Filogenia Aquaporina "Drought-induced protein Di19-like protein DIP" "fiber protein Fb2", EST Phylogeny Aquaporin Drought-induced protein Di19-like protein DIP Fiber protein Fb2
Citação
Rabello, Fernanda R.; Carazolle, Marcelo F.; Martins, Natália F.; Campos, Magnólia A.; Silva, Marilia S.; Silva, Alba C.; Souza, Alessandra A.; Amaral, Alexandre M. do; Mehta, Angela. Análise in silico de aquaporinas potencialmente envolvidas com o estresse hídrico nas interações café- e citros-Xylella fastidiosa. In: Simpósio de Pesquisa dos Cafés do Brasil (5. : Águas de Lindóia, SP : 2007). Anais. Brasília, D.F. : Embrapa - Café, 2007. (1 CD-ROM), 4p.