Viabilidade de uredosporos da ferrugem do cafeeiro (Hemileia vastatrix Berk. et Br.) sob diferentes métodos de preservação in vitro
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2005
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Este trabalho teve por objetivo avaliar a viabilidade e infectividade de uredosporos de H. vastatrix por diferentes métodos de preservação. Os tratamentos consistiram no armazenamento dos uredosporos sob diferentes temperaturas: 1) 4 ºC com 50% de umidade relativa; 2) 4 ºC após liofilização; 3) -80 ºC; 4) -20 ºC; 5) -80 ºC após congelamento em nitrogênio líquido. Utilizou-se no experimento uredosporos da raça II de H. vastatrix. A viabilidade dos uredosporos foi avaliada, pela contagem do número de uredosporos germinados em meio ágar-água a 2%. Para isso, foi espalhada uma suspensão de uredosporos (2 mg/ml), correspondente a cada tratamento, em placas de Petri que foram incubadas por 20-24 horas, a 22 ºC, e na ausência de luz. Simultaneamente, cada suspensão de esporos foi inoculada em folhas destacadas de Coffea arabica ‘Catuaí’, mantidas por 50 dias na mesma temperatura e fotoperíodo de 12 horas de luz e escuro. Os resultados mostraram que os tratamentos 3 e 5 foram os mais eficientes na preservação, mantendo a viabilidade dos uredosporos em 38% e 42%, respectivamente, oito meses após a montagem dos testes. Inoculações em folhas destacadas mostraram que o tratamento 3 foi o mais eficiente na manutenção da infectividade pelo número de lesões esporuladas.
The goal of the present work was to evaluate the viability and infectiveness of H. vastatrix uredospores by different preservation methods. The treatments consisted of uredospores storage under different temperatures: 1) 4ºC with 50% of relative humidity; 2) 4ºC after lyophilization; 3) -80ºC; 4) -20ºC; 5) -80ºC after freezing in liquid nitrogen. The H. vastatrix race II was used in the assay. The uredospores viability was evaluated, by counting the number of germinated uredospores in agar-water medium at 2%. For that, we spread uredospores suspension (2 mg/ml), corresponding to each treatment, in Petri dishes, which were incubated for 20-24 hours, at 22ºC, in the dark. Simultaneously, each uredospore suspension was inoculated in Coffea arabica ‘Catuaí’ detached leaves, maintained for 50 days at the same temperature and 12 hours of light and dark photoperiod. The results showed that the treatments 3 and 5 were the most efficient in the preservation, maintaining the uredospores viability 38% and 42% respectively for eight months. Inoculations in detached leaves showed that the treatment 3 was the most efficient in the maintenance of infectiveness of the uredospores by the number of sporulated lesions.
The goal of the present work was to evaluate the viability and infectiveness of H. vastatrix uredospores by different preservation methods. The treatments consisted of uredospores storage under different temperatures: 1) 4ºC with 50% of relative humidity; 2) 4ºC after lyophilization; 3) -80ºC; 4) -20ºC; 5) -80ºC after freezing in liquid nitrogen. The H. vastatrix race II was used in the assay. The uredospores viability was evaluated, by counting the number of germinated uredospores in agar-water medium at 2%. For that, we spread uredospores suspension (2 mg/ml), corresponding to each treatment, in Petri dishes, which were incubated for 20-24 hours, at 22ºC, in the dark. Simultaneously, each uredospore suspension was inoculated in Coffea arabica ‘Catuaí’ detached leaves, maintained for 50 days at the same temperature and 12 hours of light and dark photoperiod. The results showed that the treatments 3 and 5 were the most efficient in the preservation, maintaining the uredospores viability 38% and 42% respectively for eight months. Inoculations in detached leaves showed that the treatment 3 was the most efficient in the maintenance of infectiveness of the uredospores by the number of sporulated lesions.
Descrição
Trabalho apresentado no Simpósio de Pesquisa dos Cafés do Brasil (4. : 2005 : Londrina, PR). Anais. Brasília, D.F. : Embrapa Café, 2005.
Palavras-chave
Hemileia vastatrix Uredosporos Viabilidade Métodos de preservação Ferrugem do cafeeiro, Hemileia vastatrix Uredospores viability Preservation methods Coffee leaf rust
Citação
Capucho, Alexandre S.; Souza, Antônio F. de; Zambolim, Eunize M.; Caixeta, Eveline T.; Rufino, Raphael J. N.; Barbosa, Júlio C.; Alvarenga, Samuel M.; Zambolim, Laércio. Viabilidade de uredosporos da ferrugem do cafeeiro (Hemileia vastatrix Berk. et Br.) sob diferentes métodos de preservação in vitro. In: Simpósio de Pesquisa dos Cafés do Brasil (4. : Londrina, PR : 2005). Anais. Brasília, D.F. : Embrapa - Café, 2005. (1 CD-ROM), 4p.