Almeida, Julieta Andrea Silva deNeofti, Wilielle Cristina FreitasGiomo, Gerson Silva2022-11-282022-11-282022-11-07ALMEIDA, Julieta Andrea Silva de; NEOFTI, Wilielle Cristina Freitas; GIOMO, Gerson Silva. Osmotic potential intensity in the control of direct somatic embryogenesis in coffee tree. Pesquisa Agropecuária Brasileira, Brasília, v. 57, p. 1-4, 07 nov. 2022. Available from: https://doi.org/10.1590/S1678-3921.pab2022.v57.02947. Accessed: 25 nov. 2022.1678-3921DOI: 10.1590/S1678-3921.pab2022.v57.02947http://www.sbicafe.ufv.br/handle/123456789/13674The objective of this work was to characterize the effect of the osmotic potential of the culture medium on the control of direct somatic embryogenesis in coffee (Coffea arabica) tree. Leaf explants of the Mundo Novo cultivar and four hybrids were subjected to the direct route in the presence of polyethylene glycol 6000, at the following concentrations: 0, 2.5, 5.0, 7.5, 10.0, 12.5, and 15.0%. Embryogenic structures and somatic embryos were formed at a threshold osmotic potential of up to -0.691 and -0.374 MPa, respectively, with a reduction or inhibition in these responses under a more negative osmotic potential.pdfenOpen AccessCoffea arabicaembryogenic structurePEG 6000somatic embryoCafeicultura::Genética e melhoramentoArtigo