Santos, Tiago Benedito dosLima, Joni EsromFelicio, Mariane SilvaSoares, João Danillo MouraDomingues, Douglas Silva2022-02-082022-02-082017SANTOS, T. B. et al. Genome-wide identification, classification and transcriptional analysis of nitrate and ammonium transporters in Coffea. Genetics and Molecular Biology, Ribeirão Preto, v. 40, n. 1(suppl), p. 346-359, 2017.1678-4685http://dx.doi.org/10.1590/1678-4685-GMB-2016-0041http://www.sbicafe.ufv.br/handle/123456789/13273Nitrogen (N) is quantitatively the main nutrient required by coffee plants, with acquisition mainly by the roots and mostly exported to coffee beans. Nitrate (NO3 –) and ammonium (NH4 +) are the most important inorganic sources for N uptake. Several N transporters encoded by different gene families mediate the uptake of these compounds. They have an important role in source preference for N uptake in the root system. In this study, we performed a genome-wide analysis, including in silico expression and phylogenetic analyses of AMT1, AMT2, NRT1/PTR, and NRT2 transporters in the recently sequenced Coffea canephora genome. We analyzed the expression of six selected transporters in Coffea arabica roots submitted to N deficiency. N source preference was also analyzed in C. arabica using isotopes. C. canephora N transporters follow the patterns observed for most eudicots, where each member of theAMT andNRT families has a particular role in N mobilization, and where some of these are modulated by N deficiency. Despite the prevalence of putative nitrate transporters in the Coffea genome, ammonium was the preferential inorganic N source for N-starved C. arabica roots. This data provides an important basis for fundamental and applied studies to depict molecular mechanisms involved in N uptake in coffee trees.pdfenOpen AccessNitrogen transportNitrogen uptakeGene family evolutionCafeicultura::Genética e melhoramentoArtigo