Alves, Sandriel TrindadeDias, Rafael Carlos EloyBenassi, Marta de ToledoScholz, Maria Brígida dos Santos2022-04-072022-04-072006ALVES, S. T. et al. Metodologia para análise simultânea de ácido nicotínico, trigonelina, ácido clorogênico e cafeína em café torrado por cromatografia líquida de alta eficiência. Química Nova, São Paulo, v. 29, n. 6, p. 1164-1168, nov./dez. 2006.1678-7064https://doi.org/10.1590/S0100-40422006000600003http://www.sbicafe.ufv.br/handle/123456789/13434A reverse phase liquid chromatography method was developed for simultaneous determination of trigonelline, caffeine, nicotinic and chlorogenic (5-CQA) acids in roasted coffee. A gradient of acetic acid/acetonitrile was used as mobile phase and detection was carried out in the UV. The samples were extracted with acetonitrile/water (5:95 v/v) at 80 ºC/10 min. Good recovery (89 to 104%), repeatability and linearity were obtained. Detection limits of 0.01, 0.15, 0.04 and 0.04 mg mL-1 were observed for nicotinic acid, trigonelline, 5-CQA and caffeine. The method, applied to arabica and robusta coffees with different degrees of roasting, was efficient and fast (~35 min) and also allowed identification of cinnamic acids.pdfpt-BROpen AccessCoffea arabicaCoffea canephora5-CQACafeicultura::Qualidade de bebidaArtigo