Bellato, Ana Cláudia S.Menegário, Amauri A.Giné, Maria Fernanda2022-04-022022-04-022003BELLATO, A. C. S.; MENEGÁRIO, A. A.; GINÉ, M. F. Boron isotope dilution in cellular ractions of coffee leaves evaluated by inductively coupled plasma mass spectrometry with direct injection nebulization (DIN-ICP-MS). Journal of the Brazilian Chemical Society, São Paulo, v. 14, n. 2, p. 263-273, mar./abr. 2003.1678-4790https://doi.org/10.1590/S0103-50532003000200014http://www.sbicafe.ufv.br/handle/123456789/13413Enriched 10B (94.14 atom %) was supplied to coffee plantlets for three months. Then boron isotope ratios were determined in the leaf cell compartments, cell wall, nuclei and chloroplast, after a sub-cellular fractionation procedure. The isotopic measurements were performed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) provided with a direct injection nebulizer (DIN), introducing a sample volume of 50 µL. Isotopic ratios from 1.002 to 1.326 were determined with precision characterized by RSD lower than 1.5% for the enriched cell fractions with B concentrations ranging from 3.3 to 10.8 µg g-1. The detection limit (3σ) was 0.5 ng B mL-1. The average enrichments in 10B atom % found in the cell walls, nuclei and chloroplasts were 46.7, 44.5 and 48.8, respectively.pdfenOpen AccessIsotope dilutionInductively coupled plasma mass spectrometryBiological cell fractionationBoron isotope ratiosPlant analysisCafeicultura::Solos e nutrição do cafeeiroArtigo