Martins, Ana Carolina C. L.Silva, Tarliane M.Gloria, M. Beatriz A.2022-04-112022-04-112010MARTINS, A. C. C. L.; SILVA, T. M.; GLORIA, M. B. A. Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café. Química Nova, São Paulo, v. 33, n. 2, p. 316-320, 2010.1678-7064https://doi.org/10.1590/S0100-40422010000200016http://www.sbicafe.ufv.br/handle/123456789/13465Epidemiological studies attributed positive effects in the central nervous system (CNS) to coffee. Among possible active constituents, serotonin, a neurotransmitter in the CNS, is present; but dietary sources do not cross the blood-brain barrier. Tryptophan and 5-hidroxytryptophan (5-HTP) are serotonin precursors and can affect brain concentrations. An ion-pair-HPLC, post-column derivatization with o-phthalaldehyde and fluorimetric detection before and after hydrolysis with NaOH and extraction with methanol: water was developed for the simultaneous determination of these compounds. It was selective, sensitive (LOD = 0.3 and 0.2 mg/mL), precise (91.3 and 94.2% recovery for tryptophan and 5-HTP, respectively), and linear from 0.3 to 40 mg/mL for both compounds. It was applied to green and roasted arabica and robusta coffees.pdfpt-BROpen AccessSerotoninTryptophanCafeicultura::Café e saúdeArtigo