Brazilian Archives of Biology and Technology
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/12090
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Item Genetic diversity among forty coffee varieties assessed by rapd markers associated with restriction digestion(Instituto de Tecnologia do Paraná - Tecpar, 2005-07) Diniz, Leandro Eugênio Cardamoni; Ruas, Claudete de Fátima; Carvalho, Valdemar de Paula; Torres, Fabrício Medeiros; Ruas, Eduardo Augusto; Santos, Melissa de Oliveira; Sera, Tumoru; Ruas, Paulo MaurícioThe genetic variability of 40 accessions of C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among C. arabica genotypes.Item Partial resistance to fruit necrosis associated to Colletotrichum spp. among arabic coffee genotypes(Instituto de Tecnologia do Paraná - Tecpar, 2007-05) Sera, Gustavo Hiroshi; Sera, Tumoru; Ito, Dhalton Shiguer; Azevedo, José Alves de; Ribeiro Filho, Claudionor; Mata, João Siqueira daThe aim of this study was to evaluate the resistance to fruit necrosis associated to Colletotrichum spp. in Coffea arabica L.. Twenty-two arabic coffee genotypes were performed in a randomized block design, with three replications and ten plants per plot. They were evaluated for the variables fruit necrosis, luminosity index inside of the plant canopy and fruits per productive nodes of the plants. There was genetic variability for the resistance to fruit necrosis. Different partial resistance levels, varying from highly susceptible (score = 3.89) to moderately resistant (score = 1.77) were observed. The genotypes that presented moderate resistance were the cultivars IPR- 100, IPR-103, IPR-105 and IPR-108 and the coffee selection IAPAR-00023.