Brazilian Journal of Biology
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/13321
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Item Commercial characteristics of coffee seedlings produced with different sources of phosphorus and plant growth-promoting bacteria(Instituto Internacional de Ecologia, 2023-04-17) Ferraro, A. C.; França, A. C.; Machado, C. M. M.; Aguiar, F. R.; Oliveira, L. L.; Braga Neto, A. M; Oliveira, R. G.Considered the economic engine of many countries, the coffee culture represents an important component of the agricultural chain in Brazil. The growing values of commercialization, planting areas, and crop productivity require the acquisition of quality seedlings, which must receive adequate nutritional support through efficient fertilizers. Slow and controlled-release fertilizers, such as organominerals, gain prominence when it comes to increasing efficiency in the use of phosphorus, as well as plant growth-promoting bacteria (PGPB) with phosphate solubilizing characteristics. This study aimed to evaluate the effect of different sources of mineral and organomineral fertilizers, inoculated and non-inoculated with PGPB on the quality parameters of coffee seedlings. In general, the P sources used in the experiment positively interfered with the development of coffee seedlings. This proves that there is a need for nutritional supplementation for the good development of the seedlings. Among the sources used, the organomineral in granulated form showed better performance in coffee seedlings' growth and physiological parameters, proving to be a viable alternative to commonly used fertilizers. The addition of PGPB showed a significant advantage for seedling quality variables.Item 16S metabarcoding analysis reveals the influence of organic and conventional farming practices on bacterial communities from the rhizospheric of Coffea arabica L.(Instituto Internacional de Ecologia, 2023-11-03) Andrade, P. H. M.; Machado, P. C.; Paula, A. F.; Paganin, A. C. L.; Rezende, G. S.; Matheucci Jr., E.; Carvalho, L. M.; Freire, C. C. M.; Cunha, A. F.; Lacava, P. T.Coffea sp. is cultivated in many tropical countries. Brazil has always adopted intensive agricultural practices, but organic coffee farming is an alternative system based on the non-use of agrochemicals and the rational management of soils. Metabarcoding 16S analysis using next-generation sequencing has been developed to identify and compare the diversity of the Coffea arabica L. rhizospheric bacterial community in two farming areas in São Paulo, Brazil. Dourado uses conventional farming, while Ribeirão Corrente uses organic. We found broad taxonomic composition, with sequences from 24 phyla, 55 classes, 61 orders, 146 families, and 337genus. The three most abundant phyla were Proteobacteria (38.27%), Actinobacteria (15.56%), and Acidobacteria (16.10%). In organic farming, the top 3 were the family Sphingomonadaceae, order Rhizobiales, genus Nocardioides, and Gp6. The genus Gp2 and the phylum Candidatus Saccharibacteria were the most abundant OTUs exclusively present in conventional farming. In the organic farming practice, Proteobacteria, Actinobacteria, and Acidobacteria were also present among the exclusive OTUs; we also found OTUs belonging to Bacteroidetes, Firmicutes, and Verrucomicrobia. Our study indicates a positive effect of organic farming on microbial communities. Fertilization may directly affect soil microbiota, suggesting that a large and active microbial community low in functional diversity might not adapt to new climatic conditions. A diverse community could provide better resilience to environmental changes, improving the productivity of this important crop.Item A análise de metabarcode 16S revela a influência das práticas agrícolas orgânicas e convencionais nas comunidades bacterianas da rizosfera de Coffea arabica L.(Instituto Internacional de Ecologia, 2023-09-18) Andrade, P. H. M.; Machado, P. C.; Paula, A. F.; Paganin, A. C. L.; Rezende, G. S.; Matheucci Jr., E.; Carvalho, L. M.; Freire, C. C. M.; Cunha, A. F.; Lacava, P. T.Coffea sp. é cultivada em muitos países tropicais. O Brasil sempre adotou práticas agrícolas intensivas, mas a cafeicultura orgânica é um sistema alternativo baseado na não utilização de agrotóxicos e no manejo racional dos solos. A análise Metabarcode 16S utilizando o sequenciamento de última geração foi desenvolvida para identificar e comparar a diversidade da comunidade bacteriana rizosférica de Coffea arabica L. em duas áreas de cultivo em São Paulo, Brasil. Dourado usa agricultura convencional, enquanto Ribeirão Corrente usa agricultura orgânica. Encontramos ampla composição taxonômica, com sequências de 24 filos, 55 classes, 61 ordens, 146 famílias e 337 gêneros. Os três filos mais abundantes foram Proteobacteria (38,27%), Actinobacteria (15,56%) e Acidobacteria (16,10%). Na agricultura orgânica, os 3 primeiros foram a família Sphingomonadaceae, ordem Rhizobiales, gênero Nocardioides e Gp6. O gênero Gp2 e o filo Candidatus Saccaribacteria foram as OTUs mais abundantes exclusivamente presentes na agricultura convencional. Na prática da agricultura orgânica, Proteobacteria, Actinobacteria e Acidobacteria também estiveram presentes entre as OTUs exclusivas; também encontramos OTUs pertencentes a Bacteroidetes, Firmicutes e Verrucomicrobia. Nosso estudo indica um efeito positivo da agricultura orgânica nas comunidades microbianas. A fertilização pode afetar diretamente a microbiota do solo, sugerindo que uma grande e ativa comunidade microbiana com baixa diversidade funcional pode não se adaptar às novas condições climáticas. Uma comunidade microbiana diversificada poderia proporcionar maior resiliência às mudanças ambientais, melhorando a produtividade desta importante cultura agrícola.Item Potential of coffee straw biochal as a substrate conditioner in seed lettuce and sorghum germination and vigorit(Instituto Internacional de Ecologia, 2023) Alixandre, R. D.; Lima, P. A. M.; Almeida, T. F. R.; Oliveira, J. S.; Pereira, M. B.; Alixandre, F. T.; Jacomino, G. R. L.; Dias, R. S.; Alexandre, R. S.; Ferreira, A.; Passos, R. R.; Lopes, J. C.The use of residues from coffee production to obtain biochar is a sustainable approach, which aims to minimize the environmental impact of these materials. In this study, the effect of adding coffee straw biochar on the physiological quality of lettuce and sorghum seeds was investigated. Thus, the objective of this work was to study the effect of adding different concentrations of coffee biochar in the substrate composition on the physiological quality of lettuce (Lactuca sativa) and sorghum (Sorghum bicolor) seeds. The experimental design used was completely randomized, with five concentrations of biochar (0; 7.5; 15; 30 and 60%), conducted with four replications of 25 seeds. The use of biochar in the concentrations studied does not provide an increase in the average germination percentage and vigor of lettuce and sorghum seeds. The increase in the concentration of biochar caused less seed vigor, suggesting a toxic effect. For seed germination, there was no significant difference between lettuce and sorghum species, regardless of treatment. For the germination speed index, sorghum seeds have higher means, except for the treatment with the addition of 15% coffee straw biochar. Lettuce seeds have higher shoot length averages, except for treatment with 100% commercial substrate. The sorghum seeds have higher mean root length and dry mass than lettuce, regardless of the treatmentItem Características comerciais de mudas de café produzidas com diferentes fontes de fósforo e bactérias promotoras de crescimento de plantas(Instituto Internacional de Ecologia, 2023-03-30) Ferraro, A. C.; França, A. C.; Machado, C. M. M.; Aguiar, F. R.; Oliveira, L. L.; Braga Neto, A. M.; Oliveira, R. G.Considered the economic engine of many countries, the coffee culture represents an important component of the agricultural chain in Brazil. The growing values of commercialization, planting areas, and crop productivity require the acquisition of quality seedlings, which must receive adequate nutritional support through efficient fertilizers. Slow and controlled-release fertilizers, such as organominerals, gain prominence when it comes to increasing efficiency in the use of phosphorus, as well as plant growth-promoting bacteria (PGPB) with phosphate solubilizing characteristics. This study aimed to evaluate the effect of different sources of mineral and organomineral fertilizers, inoculated and non-inoculated with PGPB on the quality parameters of coffee seedlings. In general, the P sources used in the experiment positively interfered with the development of coffee seedlings. This proves that there is a need for nutritional supplementation for the good development of the seedlings. Among the sources used, the organomineral in granulated form showed better performance in coffee seedlings' growth and physiological parameters, proving to be a viable alternative to commonly used fertilizers. The addition of PGPB showed a significant advantage for seedling quality variables.Item Microbiological and faunal soil attributes of coffee cultivation under different management systems in Brazil(Instituto Internacional de Ecologia, 2015) Lammel, D. R.; Azevedo, L. C. B.; Paula, A. M.; Armas, R. D.; Baretta, D.; Cardoso, E. J. B. N.Brazil is the biggest coffee producer in the world and different plantation management systems have been applied to improve sustainability and soil quality. Little is known about the environmental effects of these different management systems, therefore, the goal of this study was to use soil biological parameters as indicators of changes. Soils from plantations in Southeastern Brazil with conventional (CC), organic (OC) and integrated management systems containing intercropping of Brachiaria decumbens (IB) or Arachis pintoi (IA) were sampled. Total organic carbon (TOC), microbial biomass carbon (MBC) and nitrogen (MBN), microbial activity (C-CO2), metabolic quotient (qCO2), the enzymes dehydrogenase, urease, acid phosphatase and arylsulphatase, arbuscular mycorrhizal fungi (AMF) colonization and number of spores and soil fauna were evaluated. The greatest difference between the management systems was seen in soil organic matter content. The largest quantity of TOC was found in the OC, and the smallest was found in IA. TOC content influenced soil biological parameters. The use of all combined attributes was necessary to distinguish the four systems. Each management presented distinct faunal structure, and the data obtained with the trap method was more reliable than the TSBF (Tropical Soils) method. A canonic correlation analysis showed that Isopoda was correlated with TOC and the most abundant order with OC. Isoptera was the most abundant faunal order in IA and correlated with MBC. Overall, OC had higher values for most of the biological measurements and higher populations of Oligochaeta and Isopoda, corroborating with the concept that the OC is a more sustainable system.Item The coffee berry borer: the centenary of a biological invasion in Brazil(Instituto Internacional de Ecologia, 2014) Infante, F.; Pérez, J.; Vega, F. E.Item Effects of cigarette smoke inhalation and coffee consumption on bone formation and osseous integration of hydroxyapatite implant(Instituto Internacional de Ecologia, 2013) Andrade, A. R.; Sant’Ana, D. C. M.; Mendes Junior, J. A.; Moreira, M.; Pires, G. C.; Santos, M. P.; Fernandes, G. J. M.; Nakagaki, W. R.; Garcia, J. A. D.; Lima, C. C.; Soares, E. A.The present study aims to assess the effects of cigarette smoke inhalation and/or coffee consumption on bone formation and osseous integration of a dense hydroxyapatite (DHA) implant in rats. For this study, 20 male rats were divided into four groups (n = 5): CT (control) group, CE (coffee) group, CI (cigarette) group and CC (coffee + cigarette) group. During 16 weeks, animals in the CI group were exposed to cigarette smoke inhalation equivalent to 6 cigarettes per day; specimens in the CE group drank coffee as liquid diet; and rats in the CC group were submitted to both substances. In the 6th week a 5 mm slit in the parietal bone and a 4 mm slit in the tibia were performed on the left side: the former was left open while the latter received a DHA implant. As soon as surgeries were finished, the animals returned to their original protocols and after 10 weeks of exposure they were euthanised (ethically sacrificed) and the mentioned bones collected for histological processing. Data showed that exposure to cigarette smoke inhalation and coffee consumption did not interfere in weight gain and that solid and liquid diet consumption was satisfactory. Rats in the CC group showed a decrease in bone neoformation around the tibial DHA implant (31.8 ± 2.8) as well as in boné formation in the parietal slit (28.6 ± 2.2). On their own, cigarette smoke inhalation or coffee consumption also led to diminished bone neoformation around the implant and delayed the bone repair process in relation to the CT group. However, reduction in the bone repair process was accentuated with exposure to both cigarette smoke inhalation and coffee consumption in this study.Item What is the influence of ordinary epidermal cells and stomata on the leaf plasticity of coffee plants grown under full-sun and shady conditions?(Instituto Internacional de Ecologia, 2010) Pompelli, M. F.; Martins, S. C. V.; Celin, E. F.; Ventrella, M. C.; DaMatta, F. M.Stomata are crucial in land plant productivity and survival. In general, with lower irradiance, stomatal and epidermal cell frequency per unit leaf area decreases, whereas guard-cell length or width increases. Nevertheless, the stomatal index is accepted as remaining constant. The aim of this paper to study the influence of ordinary epidermal cells and stomata on leaf plasticity and the influence of these characteristics on stomata density, index, and sizes, in the total number of stomata, as well as the detailed distribution of stomata on a leaf blade. As a result, a highly significant positive correlation (R2 a = 0.767 p ≤ 0.001) between stomatal index and stomatal density, and with ordinary epidermal cell density (R2 a = 0.500 p ≤ 0.05), and a highly negative correlation between stomatal index and ordinary epidermal cell area (R2 a = -0.571 p ≤ 0.001), were obtained. However in no instance was the correlation between stomatal index or stomatal density and stomatal dimensions taken into consideration. The study also indicated that in coffee, the stomatal index was 19.09% in shaded leaves and 20.08% in full-sun leaves. In this sense, variations in the stomatal index by irradiance, its causes and the consequences on plant physiology were discussed.Item Transformation of coffee (Coffea Arabica L. cv. Catimor) with the cry1ac gene by biolistic, without the use of markers(Instituto Internacional de Ecologia, 2010) De Guglielmo-Cróquer, Z.; Altosaar, I.; Zaidi, M.; Menéndez-Yuffá, A.The transformation of coffee plantlets with the cry1ac gene of Bacillus thuringiensis was achieved by biolistic using either the whole pUBC plasmid or only the ubi-cry1ac-nos genetic cassette. The cry1ac gene was inserted into coffee plants in order to confer resistance to the leaf miner Leucoptera coffeella, an insect responsible for considerable losses in coffee crops. Bearing in mind that the genetic cassettes used for this study lack reporter genes and/or selection marker genes, the parameters for the transformation procedure by biolistic were previously standardised with a plasmid carrying the gus reporter gene. The presence of the cry1ac gene in young plantlet tissues was determined by PCR, Southern blot and reverse transcription-PCR. Our results show that the obtainment of viable coffee plantlets, transformed by bombardment with the cry1ac gene and without selection markers nor reporter genes, is feasible.