Coffee Science_v.16, 2021
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/12727
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Item Efficiency of Metarhizium anisopliae in the control of Meloidogyne incognita in banana and coffee crops(Editora UFLA, 2021) Oliveira, Claudio Marcelo Gonçalves de; Rosa, Juliana Magrinelli Osório; Pillat, Rhayane Resende; Almeida, José Eduardo Marcondes deBanana and coffee are crops damaged by the root-knot nematode (Meloidogyne incognita). Thus, the objective of this research was to study the efficiency of biological products in the control of M. incognita in both crops. Four experiments were carried out in a greenhouse, in a completely randomized design, inoculating banana and coffee plants with 2000 eggs and second-stage juveniles of M. incognita. In experiment 1, each plot was constituted of a banana seedling cv. Prata Anã. The treatments, applied around each seedling, contained the fungi Metarhizium anisopliae, from the collection kept in the Biological Control/CAPSA, IB, and Pochonia chlamydosporia (the commercial product Rizotec). In experiment 2, coffee seedlings cv. Arara were treated in a single dose with M. anisopliae and P. chlamydosporia. In experiments 3 and 4, coffee seedlings cv. Mundo Novo were treated alone or mixed with M. anisopliaeand Purpureocillium lilacinum. Based on the results obtained after 150 days of inoculation, it was concluded that M. anisopliae and P. chlamydosporia have usage potential for biological control of M. incognita in bananas and coffee. In the coffee crop, P. chlamydosporia showed 81% efficiency in reducing the population of M. incognita, whereas for M. anisopliae, the efficiency was up to 76.9%. However, there was no difference between treatments with biological control agents in relation to fresh root weight and dry weight of the aerial part of the coffee plants.Item Comparision of different application methods to biological control Hypothenemus hampei(Editora UFLA, 2021) Pereira, Raquel Moraes Costa; Almeida, José Eduardo Marcondes de; Batista Filho, AntonioThere are several studies that deal with the problem of infestation by Hypothenemus hampei in Coffeea arabica. However, the ways of applying products based on entomopathogenic fungi are the same as those used for chemicals. The objective of the research was to comparison different methods of application of Beauveria bassiana against H. hampei. The experiments were developed in laboratory, with blastospores and B. bassiana airborne conidia powders were carried out. Application methods based on immersion, Potter Spray Tower and walking on treated surface. Were three experiments were carried out for each method. Bioassays’design comprised seven treatments (including the control), with five repetitions (each) and 25 beetles per repetition. Suspensions were standardized at 2.4×109, 1×109, 5×108, 1×108, 5×107 and 1×107conidia.mL-1. Conidia powder presented initial concentration of 1.14×1010and it was standardized at 1.14×109; 5.7×109; 1.14×108; 5.7×107; 1.14×107 and 5.7×106 conidia.g-1. A simultaneous test was also carried out based to the three application methods; treatment comprised conidia powder concentration of 1x108 and the control. Data were subjected to analysis of variance, which was followed by Tukey test (P<0.05) and, CL50, in the SPSS 12.1 software. The mortality better rates of H. hampei exposed to B. bassiana IBCB66 were1×108(immersion), 1×109 (Potter Spray Tower) and 1×108(walking), where as mean LC50were 6.1×108, 4.1×108, 2.4×108conidia.mL-1for each method, respectively. The highest mortality rate was recorded in the walking tests on the conidian powder at a concentration of 1×108 conidia.g-1 for the three repetitions of the method and also in the simultaneous test.