Tropical Plant Pathology
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/13106
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Item Characterization of Meloidogyne incognita populations from São Paulo and Minas Gerais state and their pathogenicity on coffee plants(Sociedade Brasileira de Fitopatologia, 2011) Oliveira, Dagoberto S.; Oliveira, Rosângela D’Arc Lima; Silva, Débora G.; Silva, Rodrigo V.Meloidogyne incognita is one of the most aggressive and harmful plant-parasitic nematodes attacking coffee plantations in Brazil. However, populations from Minas Gerais state (MG) do not incite disease on coffee plants as strongly as populations from São Paulo state (SP). This study aimed to compare the capacity to incite disease on coffee plants from SP and MG-populations based on penetration and post-infective development of second-stage juveniles (J2) stage. Both populations were confirmed as M. incognita by using esterase phenotype I1 and species-specific PCR. Physiologically they were classified as race 2 by differential host test. Susceptible (C. Arabica ‘Catuaí Vermelho IAC 44’) and resistant coffee seedlings (C. canephora ‘Apoatã IAC 2258’) were inoculated with J2 and assessed for penetration and development from 2nd to 40th day after inoculation. Although the penetration rate of the J2 from both populations was higher in susceptible than in resistant seedlings, the SP-population showed a higher penetration than the MG-population for both variables. Post-infective development proceeded only in individuals of the SP-population in susceptible seedlings. The incompatibility between the MG-population and coffee seedlings was evident at the penetration phase, which was also followed by post-penetration resistance factors leading to significant J2 emigration, impeding nematode establishment.Item Variabilidade in vitro, in vivo e molecular de isolados de Cercospora coffeicola(Sociedade Brasileira de Fitopatologia, 2011) Acqua, Raphaela Dell’; Mantovani, Elaine Spindola; Braghini, Masako T.; Oliveira, Claudio Marcelo G.; Harakava, Ricardo; Robaina, Abikeyla S.; Petek, Marcos Rafael; Patricio, Flávia R. A.Neste estudo, sete isolados de Cercospora coffeicola, agente causal da cercosporiose em cafeeiros, foram estudados com relação ao crescimento micelial em diferentes temperaturas, comparação das sequências das regiões ITS1 e ITS2 do DNA ribossômico e de parte do gene da calmodulina, e com relação à patogenicidade a oito cultivares de cafeeiro (Catuaí Vermelho IAC 81, Catuaí SH3, Tupi IAC 1669 – 33, Tupi RN IAC 1669 – 13, Icatu Vermelho IAC 4045, Ouro Verde IAC H 5010-5, Geisha IAC 1137 e Mundo Novo IAC 376-4). As temperaturas de maior crescimento micelial foram de 23,8, 23,7 e 23,9°C para os isolados IBLF277 (Franca, SP), IBLF280 (São Sebastião do Paraíso, MG) e IBLF379 (São João do Manhuaçu, MG), respectivamente; de 24,6°C e de 25,1°C para os isolados IBL199 (Patos de Minas, MG) e IBLF270 (Boa Esperança, MG); e de 27,6 e 27,8°C, para os isolados IBLF004 (Campinas, SP) e IBLF206 (Patrocínio, MG). Na análise filogenética os isolados IBLF270 e IBLF379 formaram um subgrupo único, mas as sequências do gene da calmodulina de todos os isolados apresentaram 100% de identidade. Os isolados IBLF277, IBLF270, IBLF379 e IBLF199 foram os mais patogênicos às mudas de cafeeiro das diferentes cultivares. A cultivar Ouro Verde IAC H 5010-5 foi a mais resistente à cercosporiose e as cultivares Geisha IAC 1137, Mundo Novo IAC 376-4 e Tupi IAC 1669-33 as mais suscetíveis, entretanto, no segundo experimento, houve interação entre cultivares e isolados.Item Reaction of coffee genotypes to different populations of Meloidogyne spp.: detection of a naturally virulent M. exigua population(Sociedade Brasileira de Fitopatologia, 2009) Muniz, Maria de Fátima S.; Campos, Vicente P.; Moita, Antônio W.; Gonçalves, Wallace; Almeida, Maria Ritta A.; Sousa, Fábio Rodrigues de; Carneiro, Regina Maria D. G.The reaction of seven genotypes of Coffea arabica to 10 Meloidogyne spp. populations collected mainly from coffee plantations in Brazil and Costa Rica was evaluated under greenhouse conditions. The inoculum consisted of 10,000 eggs per plant. Evaluations were done 8 months after inoculations considering the root fresh weight, gall and egg mass indices, number of eggs per gram of root and reproduction factor (RF). The cultivars Obatã IAC 1669-20, Sarchimor IAC 4361 and Tupi Amarelo IAC 5111 exhibited susceptibility to the four Brazilian M. exigua populations tested. However, cv. Tupi Vermelho IAC 1669-33 revealed resistance (RF value of 0.7) to the M. exigua population from Lavras, Minas Gerais State, Brazil. A population of M. exigua from Bom Jesus de Itabapoana, Rio de Janeiro State, Brazil, was highly virulent on cv. IAPAR 59 (RF= 165.7), bearing resistance gene Mex-1, and was also virulent on genotype Paraíso (H 419-5-4-5-2) (RF=396.2). A Meloidogyne sp. population on coffee from Garça, São Paulo State, Brazil, reproduced at low rates (RF ranging from 0.1 to 3.9) on all genotypes. All tested cultivars were susceptible to M. incognita and M. paranaensis. M. mayaguensis of guava from Paraná State, Brazil, reproduced at low rates in all coffee genotypes; however, another population of coffee, from Costa Rica, was more aggressive and showed RF value that ranged from 0.8 to 12.4. Results of this study point for the first time to the ability of a naturally occurring M. exigua population to overcome the resistance conferred by the Mex-1 gene.Item Pathogenicity of Meloidogyne spp. (Tylenchida: Meloidogynidae) from Brazil and Central America on two genotypes of Coffea arabica(Sociedade Brasileira de Fitopatologia, 2008) Carneiro, Regina M.D.G.; Mesquita, Luiz F.G. de; Gonçalves, Wallace; Pereira, Antônio A.Entre as espécies de nematóides de galhas mais danosas ao cafeeiro, Meloidogyne exigua, Meloidogyne paranaensis, Meloidogyne incognita, Meloidogyne arabicida, Meloidogyne izalcoensis e Meloidogyne mayaguensis são as que causam os maiores problemas em áreas de cultivo de café no Brasil e países da América Latina. A reação de resistência a essas seis epécies foi estudada na progênie H419-5-4-5-2, obtida do cruzamento artificial entre cultivar Catuaí Amarelo IAC 30 com a seleção de Híbrido de Timor UFV 445-46.