Hoehnea
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/13550
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Item Influência da alta concentração atmosférica de CO2 (↑[CO2 ]atm) × disponibilidade hídrica nas relações hídricas, trocas gasosas e acúmulo de carboidratos em Coffea arabica L.(Instituto de Pesquisas Ambientais, 2017) Sanches, Rodrigo Fazani Esteves; Catarino, Ingrid Cristina Araujo; Braga, Marcia Regina; Silva, Emerson Alves da(Influência da alta concentração atmosférica de CO2 (↑[CO2]atm) × disponibilidade hídrica nas relações hídricas, trocas gasosas e acúmulo de carboidratos em Coffea arabica L.). O objetivo deste trabalho foi avaliar a influência da ↑[CO2]atm nas relações hídricas, trocas gasosas e acúmulo de carboidratos em Coffea arabica. Cafeeiros foram cultivados sob diferentes [CO2]atm (400 e 760 ppm) e submetidos a dois regimes hídricos: rega diária e ciclos de suspensão de regas por 7, 10, 14 e 37 dias, seguidos de reidratação diária por 7 dias. Alterações significativas no potencial hídrico foram observadas nas plantas sob restrição hídrica a partir do 10º dia com recuperação total após a reidratação. As A foram maiores nos cafeeiros cultivados sob ↑[CO2]atm, mesmo sob restrição hídrica se comparadas aos demais tratamentos. Cafeeiros cultivados em ↑[CO2]atm e restrição hídrica não apresentaram diminuição nos teores de carboidratos em comparação aos cultivados sob ↑[CO2]atm e rega diária. Entretanto, em plantas sob 400 ppm CO2 e restrição hídrica, reduções significativas nos teores de açúcares, principalmente em folhas, foram observadas. Os teores de amido não alteraram em resposta aos tratamentos de CO2 e água. Aumento no nível de prolina foi observado no início da restrição hídrica retornando a níveis basais ao longo do experimento. Nossos resultados apontam para um efeito mitigador do ↑CO2 sobre o déficit hídrico por meio da manutenção no acúmulo de carboidratos das plantas de café.Item Influência da alta concentração atmosférica de CO2 (↑[CO2]atm) × disponibilidade hídrica nas relações hídricas, trocas gasosas e acúmulo de carboidratos em Coffea arabica L.(Instituto de Pesquisas Ambientais, 2017) Sanches, Rodrigo Fazani Esteves; Catarino, Ingrid Cristina Araujo; Braga, Marcia Regina; Silva, Emerson Alves daThe aim of this work was to evaluate the combined effects of ↑[CO2]atm and water availability in water relations, gas exchange and carbohydrate accumulation in Coffea arabica L. Plants were cultivated under 400 and 760 ppm of CO2 in open top chambers. For each [CO2]atm, plants were divided into two groups and submitted to the following water regimes: daily watering and withholding watering for 7, 10, 14 and 37 days, followed by daily rehydration for 7 days. From day 10 on, significant changes in leaf water potential were observed in plants under water suppression in both treatments, with total recovery after rehydration. The A was higher in plants submitted to ↑[CO2]atm, even under water suppression, compared to the daily watering regime. No change in the leaf carbohydrate content was observed in plants cultivated under ↑[CO2]atm and water restriction when compared to those under ↑[CO2]atm and daily watered treatment. However, plants submitted to 400 ppm CO2 and water restriction showed a significant decrease in the sugar content, mainly in the leaves. Starch contents did not change in response to CO2 and water treatments. An increase in the rates of leaf proline was observed at the beginning of water restriction, but decreased throughout the experiment. Our results indicate a mitigating effect of the ↑CO2 on water deficit through the maintenance of carbohydrate accumulation of coffee plants.Item Antioxidative responses of cell suspension cultures of two Coffea arabica varieties to low aluminum levels at pH 5.8(Instituto de Pesquisas Ambientais, 2012) Bottcher, Alexandra; Nobile, Paula Macedo; Martins, Paula Fabiane; Conte, Fábio Frangiotti; Azevedo, Ricardo Antunes; Mazzafera, PauloThe effects of aluminum (Al) on the activities of antioxidant enzymes and ferritin expression were studied in cell suspension cultures of two varieties of Coffea arabica, Mundo Novo and Icatu, in medium with pH at 5.8. The cells were incubated with 300 μM Al3+, and the Al speciation as Al3+ was 1.45% of the mole fraction. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) were increased in Mundo Novo, whereas glutathione reductase (GR) and guaiacol peroxidase (GPOX) activities remained unchanged. SOD, GR, and GST activities were increased in Icatu, while CAT activity was not changed, and GPOX activity decreased. The expression of two ferritin genes (CaFer1 and CaFer2) were analyzed by Real-Time PCR. Al caused a downregulation of CaFER1 expression and no changes of CaFER2 expression in both varieties. The Western blot showed no alteration in ferritin protein levels in Mundo Novo and a decrease in Icatu. The differential enzymes responses indicate that the response to Al is variety-dependent.