Scientia Agrícola
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/12094
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Item Invertase and sucrose synthase activities in coffee plants sprayed with sucrose solution(Escola Superior de Agricultura "Luiz de Queiroz", 2003-04) Silva, José Carlos da; Alves, José Donizeti; Alvarenga, Amauri Alves de; Magalhães, Marcelo Murad; Livramento, Dárlan Einstein do; Fries, Daniela DeitosOne management practice of which the efficiency has not yet been scientifically tested is spraying coffee plants with diluted sucrose solutions as a source of carbon for the plant. This paper evaluates the effect of foliar spraying with sugar on the endogenous level of carbohydrates and on the activities of invertase and sucrose synthase in coffee (Coffea arabica L.) seedlings with reduced (low) and high (normal) levels of carbon reserve. The concentrations used were 0.5 and 1.0% sucrose, and water as a control. The use of sucrose at 1.0% caused an increase in the concentration of total soluble sugars in depauperate plants, as well as increased the activity of the following enzymes: cell wall and vacuole acid invertase, neutral cytosol invertase and sucrose synthase. In plants with high level of carbon reserve, no increments in total soluble sugar levels or in enzymatic activity were observed. Regardless of treatments or plants physiological state, no differences in transpiration or stomatal conductance were observed, demonstrating the stomatal control of transpiration. Photosynthesis was stimulated with the use of 0.5 and 1.0 % sucrose only in depauperate plants. Coffee seedling spraying with sucrose is only efficient for depauperate plants, at the concentration of 1.0%.