Scientia Agrícola
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/12094
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Item Initial performance and genetic diversity of coffee trees cultivated under contrasting altitude conditions(Escola Superior de Agricultura "Luiz de Queiroz", 2023-08-14) Senra, João Felipe de Brites; Silva, Josimar Aleixo da; Ferreira, Adésio; Esposti, Marlon Dutra Degli; Ferrão, Maria Amélia Gava; Fassarella, Kamila Machado; Silva, Uliana Ribeiro; Milheiros, Idalina Sturião; Silva, Fernanda Gomes daThis work evaluated the initial performance and genetic diversity of Coffea canephora genotypes cultivated in environments at contrasting altitudes. Fourteen morphophysiological traits and seven descriptors of the genus Coffea spp. of coffee trees cultivated at altitudes of 140 m and 700 m were evaluated. The design used was Federer’s augmented block in a 2 × 112 factorial scheme with six blocks. The first factor was the two environments, and the second was the 112 genotypes, with eight common treatments, being five conilon coffee clones and three arabica coffee cultivars. The data were analyzed by the method of REML/BLUP and genetic correlation method. Genetic diversity was evaluated by estimating the distance matrix, applying the Gower methodology followed by the clustering method by Tocher and UPGMA. The phenotypic means were higher in the environment at an altitude of 700 m, except for plant height, number of leaves, and canopy height (CH). Genotypic effects were significant for most traits except for leaf width, CH, unit leaf area, and total leaf area. A wide genetic diversity was verified, with distances varying from 0.037 to 0.593 for the pairs of genotypes 26 × 93 and T7 × 76, respectively. Most of the traits studied showed high genotypic correlation with the environment and expressive genetic correlation between the evaluated traits thereby demonstrating the possibility of indirect selection. There is an adaptation of conilon coffee genotypes to high altitudes and the possibility of developing a specific cultivar for the southern state of Espírito Santo.Item Receptor-Like Kinase (RLK) as a candidate gene conferring resistance to Hemileia vastatrix in coffee(Escola Superior de Agricultura "Luiz de Queiroz", 2021) Almeida, Dênia Pires de; Castro, Isabel Samila Lima; Mendes, Tiago Antônio de Oliveira; Alves, Danúbia Rodrigues; Barka, Geleta Dugassa; Barreiros, Pedro Ricardo Rossi Marques; Zambolim, Laércio; Sakiyama, Ney Sussumu; Caixeta, Eveline TeixeiraThe biotrophic fungus Hemileia vastatrix causes coffee leaf rust (CLR), one of the most devastating diseases in Coffea arabica. Coffee, like other plants, has developed effective mechanisms to recognize and respond to infections caused by pathogens. Plant resistance gene analogs (RGAs) have been identified in certain plants as candidates for resistance (R) genes or membrane receptors that activate the R genes. The RGAs identified in different plants possess conserved domains that play specific roles in the fight against pathogens. Despite the importance of RGAs, in coffee plants these genes and other molecular mechanisms of disease resistance are still unknown. This study aimed to sequence and characterize candidate genes from coffee plants with the potential for involvement in resistance to H. vastatrix. Sequencing was performed based on a library of bacterial artificial chromosomes (BAC) of the coffee clone ‘Híbrido de Timor’ (HdT) CIFC 832/2 and screened using a functional marker. Two RGAs, HdT_ LRR_RLK1 and HdT_LRR_RLK2, containing the motif of leucine-rich repeat-like kinase (LRR-RLK) were identified. Based on the presence or absence of the HdT_LRR_RLK2 RGA in a number of differential coffee clones containing different combinations of the rust resistance gene, these RGAs did not correspond to any resistance gene already characterized (SH1-9). These genes were also analyzed using qPCR and demonstrated a major expression peak at 24 h after inoculation in both the compatible and incompatible interactions between coffee and H. vastatrix. These results are valuable information for breeding programs aimed at developing CLR-resistant cultivars, in addition to enabling a better understanding of the interactions between coffee and H. vastatrix.Item Genomic prediction of leaf rust resistance to Arabica coffee using machine learning algorithms(Escola Superior de Agricultura "Luiz de Queiroz", 2021) Sousa, Ithalo Coelho de; Nascimento, Moysés; Silva, Gabi Nunes; Nascimento, Ana Carolina Campana; Cruz, Cosme Damião; Silva, Fabyano Fonseca e; Almeida, Dênia Pires de; Pestana, Kátia Nogueira; Azevedo, Camila Ferreira; Zambolim, Laércio; Caixeta, Eveline TeixeiraGenomic selection (GS) emphasizes the simultaneous prediction of the genetic effects of thousands of scattered markers over the genome. Several statistical methodologies have been used in GS for the prediction of genetic merit. In general, such methodologies require certain assumptions about the data, such as the normality of the distribution of phenotypic values. To circumvent the non-normality of phenotypic values, the literature suggests the use of Bayesian Generalized Linear Regression (GBLASSO). Another alternative is the models based on machine learning, represented by methodologies such as Artificial Neural Networks (ANN), Decision Trees (DT) and related possible refinements such as Bagging, Random Forest and Boosting. This study aimed to use DT and its refinements for predicting resistance to orange rust in Arabica coffee. Additionally, DT and its refinements were used to identify the importance of markers related to the characteristic of interest. The results were compared with those from GBLASSO and ANN. Data on coffee rust resistance of 245 Arabica coffee plants genotyped for 137 markers were used. The DT refinements presented equal or inferior values of Apparent Error Rate compared to those obtained by DT, GBLASSO, and ANN. Moreover, DT refinements were able to identify important markers for the characteristic of interest. Out of 14 of the most important markers analyzed in each methodology, 9.3 markers on average were in regions of quantitative trait loci (QTLs) related to resistance to disease listed in the literature.Item Clonal Arabica coffee resistant to Meloidogyne paranaensis and damage threshold on plants development(Escola Superior de Agricultura "Luiz de Queiroz", 2019-05) Fatobene, Bárbhara Joana dos Reis; Gonçalves, Wallace; Oliveira, Claudio Marcelo Gonçalves; Guerreiro Filho, OliveiroPlants of Coffea arabica Icatu IAC 925 germplasm show resistance to Meloidogyne paranaensis under field conditions, but with variable rates of segregation. One mother plant, IAC 3, of Icatu IAC 925 was evaluated under controlled conditions through the reaction of its clones, obtained by somatic embryogenesis. Reproduction of nematodes in clones were compared with an open-pollinated progeny from the same mother plant, C. arabica cultivar Mundo Novo IAC 515-20, susceptible to nematodes, and Clone IAC 1, also susceptible and obtained by somatic embryogenesis. Open-pollinated progeny showed segregation to resistance and was classified as susceptible according to the reproduction factor. The average number of nematodes (eggs and J2) per gram of roots in clones was eight fold lower than that of open-pollinated progeny, and 51 fold lower than Mundo Novo IAC 515-20. Based on Seinhorst model, IAC 3 clone showed a tolerance limit of 7.7 nematodes per cm3 of soil, while in Mundo Novo the tolerance limit was 3.9 nematodes per cm3 of soil. These results revealed that clonal Arabica coffee nematode- resistant trees represent an alternative to the time consuming and expensive process inherent to conventional breeding strategies.Item Histopathological characterization of Coffea arabica cultivar IPR 106 resistance to Meloidogyne paranaensis(Escola Superior de Agricultura "Luiz de Queiroz", 2019-09) Shigueoka, Luciana Harumi; Dorigo, Orazília França; Arita, Lucas Yuuki; Fonseca, Inês Cristina de Batista; Silva, Santino Aleandro da; Sera, Gustavo Hiroshi; Machado, Andressa Cristina ZamboniMeloidogyne species are widely distributed in coffee growing areas in Brazil, where M. paranaensis is responsible to significant losses to the crop. One of the main management strategies is the use of resistant cultivars, such as Apoatã rootstock and cultivars IPR 100 and IPR 106. However, the parasitic relationship between coffee and M. paranaensis in culti- vars carrying resistance genes has been poorly understood. The objective of this paper was to characterize the resistance response of Coffea arabica cv. IPR 106 to M. paranaensis. For this, penetration and histopathological studies were carried out using root tissues parasitized by the nematode. ‘IPR 106’ and ‘Mundo Novo’ (susceptible cultivar) seedlings were inoculated with 4,000 eggs of M. paranaensis and, 15 days after inoculation (DAI), roots were stained with fuch- sine to verify the penetration rates of the nematode. Histopathological studies were conducted at 15, 30, 45 and 60 DAI and nematode reproduction was calculated at 120 DAI. ‘IPR 106’ did not avoid completely nematode penetration at 15 DAI, although the number of nematodes was 50 % lower than in Mundo Novo, disregarding the hypothesis of a pre-infectional mechanism of resistance that could prevent nematode penetration completely. However, giant cells and col- lapsed nematodes were observed in ‘IPR 106’ at 30 DAI, suggesting that the resistance mecha- nism to M. paranaensis in ‘IPR 106’ involves a post-infectional response and could be mediated by a hypersensitive reaction.Item Genetic diversity of cultivated Coffea arabica inbred lines assessed by RAPD, AFLP and SSR marker systems(Escola Superior de Agricultura "Luiz de Queiroz", 2005-07) Maluf, Mirian Perez; Silvestrini, Milene; Ruggiero, Luciana Machado de Campos; Guerreiro Filho, Oliveiro; Colombo, Carlos AugustoOne of the greatest problems in Coffea arabica breeding is identifying precisely any inbred line, based only on botanical and agronomical descriptors, because of the reduced genetic variability of the species, close pedigree origin, which results in small phenotypic variation. Recently, molecular markers have been used for plant germplasm characterization and identification in several commercial species. This work evaluates the reliability of three marker systems: RAPD, AFLP and SSR, to characterize the genetic variability of commercially-used Coffea inbred lines developed by the Instituto Agronômico (IAC), and their potential for cultivar identification. All methods identified polymorphisms among the cultivars. The genetic diversity recognized by the methods is very similar, although is very narrow. RAPD and SSR marker systems grouped more efficiently the evaluated cultivars according to parental origin. None of the methods allowed inbred line identification. Therefore for varietal protection, it would be necessary using a combination of botanical, agronomical and molecular markers descriptors for precise cultivar identification.