Journal of Seed Science

URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/13100

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Antioxidant and antimicrobial activity of cathode and anode water in Coffea arabica L. seeds
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2021) Vilela, Ana Luiza Oliveira; Rosa, Sttela Dellyzete Veiga Franco da; Coelho, Stefânia Vilas Boas; Fávaris, Nathália Aparecida Bragança; Fantazzini, Tatiana Botelho; Baute, Júlia Lima
Seed deterioration induces the formation of free radicals and proliferation of microorganisms, but the application of external antioxidants may lessen these effects. The aim of this study was to investigate the antioxidant and antimicrobial activity of cathode and anode water on the physiological quality and health of Coffea arabica L. seeds. Part of the seeds were soaked in cathode water and the other part in anode water for periods of 1, 6, 12, 24, and 36 hours. The physiological quality of the seeds was evaluated by the germination test and tetrazolium test, and seed health by the Blotter test. Analysis of variance and Pearson correlation analysis were performed on the data for all the response variables. Soaking in cathode water or anode water up to 24 hours improves the physiological quality of coffee seeds. Anode water has a beneficial effect on the health of coffee seeds, reducing the incidence of Fusarium spp., Penicillium spp., Cladosporium spp., and Alternaria spp. after one hour of soaking and of Aspergillus spp. after six hours. Soaking in cathode water for a period of one hour leads to a reduction in the fungi Aspergillus spp., Fusarium spp., Cladosporium spp., and Alternaria spp., and reduction in Penicillium spp. after six hours.
Association between the artificial aging test and the natural storage of coffee seeds
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2018) Fantazzini, Tatiana Botelho; Rosa, Sttela Dellyzete Veiga Franco da; Pereira, Cristiane Carvalho; Pereira, Diego de Sousa; Cirillo, Marcelo Ângelo; Ossani, Paulo César
The accelerated aging test is recognized as an efficient method for evaluating the vigor of seed lots and for estimating their storage potential. Thus, this work aimed to evaluate the association between artificial aging and natural storage of coffee seeds, through the correlation factor analysis. Seeds of four cultivars of Coffea arabica L. (Catuaí Amarelo, Arara, Catiguá, and Mundo Novo) and one of Coffea canephora Pierre (Apoatã) were used. Part of the newly-harvested seeds were aged in a growth chamber under controlled temperature and relative humidity conditions (42 ºC and 100% RH) for periods of 0, 4, 6, 8, and 10 days. The other part of the seeds was stored in tri-wall paper packaging for a period of 2, 4, and 6 months in a non-climate-controlled environment. Artificial aging allows predictions on the storage potential of coffee seeds, although the artificial aging periods depend on the cultivars.
Behavior of coffee seeds to desiccation tolerance and storage
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2014) Abreu, Luciana Aparecida de Souza; Veiga, Adriano Delly; Pinho, Édila Vilela de Resende Von; Monteiro, Fiorita Faria; Rosa, Sttela Dellyzette Veiga Franco da
The technology developed by breeding programs is applied to coffee seeds; however, after processing and drying, they lose viability within a short period of time, thus making storage unsuitable. The objective of this research was to evaluate the quality of coffee seeds submitted to different drying methods and moisture contents during storage. The coffee seeds were submitted to conventional drying (slow shade drying) and fast drying in a static drier until they reached a moisture content of 40, 20, 12 and 5%. After this process, the seeds were stored in a cold chamber for 12 months, and seed quality was evaluated before and during storage by the germination test, electrophoretic patterns of heat resistant proteins, and the activity of isoenzyme systems. Conventional drying (slow shade drying) at 20% of moisture content maintains coffee seed quality until 12 months of storage.
Desiccation sensitivity from different coffee seed phenological stages
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2014) Santos, Flávia Carvalho; Rosa, Sttela Dellyzete Veiga Franco da; Pinho, Édila Vilela de Rezende Von; Cirillo, Marcelo Angelo; Clemente, Aline da Consolação Sampaio
Maturity stage and drying method are the factors that most influence coffee seed quality. The objective of this study was to assess the physiological quality and investigate the electrophoretic patterns of catalase and endo-ß-mannanase enzymes and heat resistant proteins in coffee seeds harvested at different phenological stages and dried under different conditions. Physiological quality was assessed when the seeds had developed the green, greenish-yellow, cherry, overripe and dry stages after three treatments: no drying, conventional drying and fast drying. After each treatment, the physiological quality of the seeds was assessed using the germination test and electrophoretic patterns of heat resistant proteins and the activity of catalase and endo-ß-mannanase enzymes. Seeds harvested at the cherry phenological stage had the best physiological quality, and the drying process reduced quality at the cherry, overripe and dry stages. This reduction was greater under the faster drying process, but at the greenish-yellow stage, seeds had better physiological quality after slow drying. Regarding the results from electrophoretic analysis, endo-ß-mannanase and catalase activities increase as the ripeness stages advance; the activity of endo-ß-mannanase is directly associated with the deterioration process; the expression of heat resistant proteins increases with maturation process and is associated with seed physiological quality.
Digital images of seedling for evaluating coffee seed vigor
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2019) Trujillo, Heiber Andres; Gomes-Junior, Francisco Guilhien; Cicero, Silvio Moure
The digital image analysis of seedlings has become largely employed in seed quality-control programs due to its feasibility, objectivity and fast results. Despite these advantages, no studies have yet demonstrated the efficiency of this technique for analyzing coffee seed vigor. The present study aimed at evaluating the efficiency of the Seed Vigor Imaging System (SVIS®) in determining the vigor of coffee seeds (Coffea arabica L.) from digital images of seedlings. Also, the results of these analyses were contrasted with conventional vigor tests. Six seed lots from each cultivar, Bourbon and Catucaí 20/15, were used. The research was conducted in two experimental times, and the seed vigor was determined by the tests of first germination count, accelerated aging, electrical conductivity, primary root protrusion speed, as well as by the analysis of scanned images of seedlings, made possible by the SVIS® software. For the Bourbon and Catucaí 20/15 cultivars, the SVIS® analysis was able to discriminate lots of both high and low vigor (vigor indexes of 317 to 752, and 181 to 703, respectively). A similar outcome was obtained from the conventional tests appraised in this research. Eventually, it was possible to conclude that the digital analysis of 20-day-old seedlings by SVIS® is an efficient method to evaluate coffee seed vigor.
Effect of chemical treatment on the physiological and sanitary quality of stored coffee seeds
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2021) Penido, Amanda Carvalho; Rodrigues, Vitor Oliveira; Carvalho, Marcos Vinícios de; Krepischi, Levi Suzigan; Pereira, Cristiane Carvalho; Oliveira, João Almir
Maintaining the health of coffee seeds is especially important during storage, as soil fungi and storage fungi can considerably reduce seed quality. Thus, chemical treatments for protection of seeds in storage becomes important in agricultural production. It is necessary to evaluate the effects of these treatments on seedling development and the protection they provide against storage fungi, aiming at seed longevity and preventing rapid deterioration. The aim of this study was to evaluate the effect of chemical treatment on the physiological and sanitary quality of stored coffee seeds. Seeds of five Coffea arabica cultivars were pre-dried, treated with Vitavax®- Thiram, and placed in cold storage at 10 °C for nine months. Seed physiological quality was evaluated every three months by the germination test and by determination of root emergence percentage, seedlings with expanded cotyledonary leaves, and seedling dry matter. Seed health quality was assessed by the health test. The chemical treatment with Vitavax-Thiram does not affect the physiological quality of stored Coffea arabica seeds. Seed treatment before storage is effective in reducing the inoculum potential of Fusarium spp. and Phoma spp. in coffee seeds.
Effect of physiological priming on stored coffee seeds
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2021) Penido, Amanda Carvalho; Rezende, Édila Maria de; Pereira, Diego de Sousa; Reis, Venícius Urbano Vilela; Rocha, Debora Kelli; Oliveira, João Almir
The physiological priming has been used to standardize and increase the speed of development of seed lots. The use of this technique is essential in coffee cultivation, as the crop has low longevity associated with slow and uneven germination. The objective was to evaluate the effect of physiological priming on the physiological quality of coffee seeds stored with different water contents. Seeds of two cultivars of Coffea arabica were used: cv. Catuaí Vermelho IAC144 and Topázio MG1190. A portion of the seeds were dried in the shade until reaching 12% moisture, and the other did not undergo drying. The seeds were stored in a cold chamber at 10 °C for nine months. Every three months, the physiological priming technique was performed with subsequent evaluation of the physiological quality by germination tests, root protrusion, normal seedlings at fifteen days, strong normal seedlings, seedlings with expanded cotyledonary leaves and seedling dry weight. Seeds that did not undergo drying subjected to the physiological priming technique maintained physiological quality after nine months of storage. Physiological priming was detrimental to dry seeds stored for nine months.
Exploratory studies for cryopreservation of Coffea arabica L. seeds
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2017) Figueiredo, Madeleine Alves de; Coelho, Stefania Vilas Boas; Rosa, Sttela Dellyzete Veiga Franco da; Vilela, Amanda Lima; Silva, Luciano Coutinho
Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling.
Exudate coloring test suitability for assessing the viability of coffee seeds (Coffea arabica L.)
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2016) Hilst, Paulo César; Dias, Denise Cunha Fernandes dos Santos; Gama, Guilherme Fontes Valory; Araújo, Joyce de Oliveira
The exudate coloring test has been promising in order to quickly evaluate the quality of coffee seeds. The objective of the research was to adjust the coloring exudate test for coffee seeds and to evaluate the influence of the water content of seeds and of the imbibition period on the test results. Seeds from five lots of ‘Catuaí 44’ were used, with the following water contents: 30%, 20% and 12%. For the exudate coloring test, the parchment and silver skin (spermoderm) from the seeds were removed. Then, the seeds were distributed on a paper towel, moistened with water, and kept in a germinator at 25 °C for 24, 48, 72, 96 and 120 h. Four classes of coloring intensity were established: absence of color (A), light (L), medium (M) and strong (S) intensities, assigning the values 0, 3, 5 and 10 for each class, respectively. The Viability Index (VI) was calculated by the equation VI=100-(0xA)-(3xL)-(5xM)-(10xS). The exudate coloring test may be recommended to estimate the viability of coffee seeds, providing results correlated to the germination test. The best results were obtained for the seeds with 12% moisture content imbibed for 72, 96 and 120 h and seeds with 30% moisture content after 72 and 120 h of imbibition.
Gene expression during the germination of coffee seed
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2019) Silva, Edvaldo Aparecido Amaral da; Acencio, Marcio Luis; Bovolenta, Luiz Augusto; Lemke, Ney; Varani, Alessandro de Mello; Bravo, Juliana Pereira; Hoshino-Bezerra, Andrea Akemi; Lemos, Eliana Gertrudes Macedo
Germination of the coffee (Coffea arabica L.) seed is the result of events that occur simultaneously in the embryo and endosperm. To understand the molecular mechanisms responsible for these events, we undertook a transcriptome analysis of embryo, micropylar and lateral endosperms from 10-day-imbibed seeds. The sequencing yielded contigs coding for 16,813 proteins. From those, 14,005 (~ 83%) were highly similar to at least one protein sequence in the nr database. 162 genes were significantly expressed in the embryo, 36 in the micropylar endosperm and 72 in the lateral endosperm. The tissue specificity analysis of the significantly expressed genes showed that the embryo had the highest proportion of specific genes (113/162, ~70%), while 11 were expressed in the micropylar and lateral endosperms. In the embryo, genes were mainly associated with abiotic stress, cell growth, and intercellular communication. In the micropylar and lateral endosperms, they were associated with abiotic stress and cell wall degradation. The accuracy of RNA-seq data was confirmed by RT-qPCR. This work adds new information about the molecular mechanism involved in coffee seed germination.
Minimum period to assess the potential of germination of coffee seeds
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2013) Guimarães, Gabriel Castanheira; Rosa, Sttela Dellyzette Veiga Franco da; Coelho, Luis Filipe Serafim; Veiga, Adriano Delly; Clemente, Aline da Consolação Sampaio
In recent research, early stages of coffee seedling development were identified, after radicle protrusion, in which all the essential parts of the seedling can be assessed to demonstrate the potential of seed germination to generate normal plants. Thus, the objective of this research was to develop studies to reduce the time on the germination test. Ten seed lots of coffee were assessed through the germination test, every two days, from the tenth day of sowing, according to the morphological criteria of the seedlings. The data were compared to the standard germination test and analyzed by means of regression analysis and segmented nonlinear regression and planteau response model. It was concluded that the assessment of coffee seedlings at 16 days, in stage S1, provides the similar result of the pattern germination test, allowing a significant reduction of time in order to demonstrate the germination of the seed lots; the seedling assessment coffee in stage S2 does not have reduction of the time for assessing the coffee germination, in relation to the pattern.
Protein profile in arabica coffee seeds in electrophoresis gel: importance of freeze-drying
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2022-05-13) Fávaris, Nathália Aparecida Bragança; Rosa, Sttela Dellyzete Veiga Franco da; Figueiredo, Madeleine Alves de; Coelho, Stefânia Vilas Boas; Vilela, Ana Luiza de Oliveira; Padilha, Lilian
Coffee seeds are sensitive to desiccation and are used or stored with different moisture content values, which may affect the results of quality assessment. The aim of this study was to evaluate the changes in protein profile in electrophoresis gel in coffee seeds with different moisture content values under freeze-drying and without freeze-drying. Two lots of arabica coffee seeds were used, one of newly-harvested seeds and another of stored seeds. The seeds were dried to the moisture content values of 12, 15, 20, 25, 30, 35, and 40%. The physiological quality of the seeds was assessed through the germination test, electrical conductivity, and the profiles of the enzymes SOD, CAT, PO, GOT, MDH, and EST and of heat-resistant proteins. In general, there is an effect on expression of these enzymes in accordance with the presence of free water in the seeds. Moist seeds have little to no enzyme expression. The freeze-drying process allows preservation of coffee seed quality and does not change the functionality of the enzymes studied. The isoenzyme profiles of the antioxidant process in arabica coffee seeds are affected by the initial moisture content of the seeds. The freeze-drying process of the seeds ensures greater sensitivity in detection of the expression of these isoenzymes.
Radiographic analysis and performance of coffee seeds
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2019) Trujillo, Heiber Andres; Gomes-Junior, Francisco Guilhien; Lara, Idemauro Antonio Rodrigues de; Cicero, Silvio Moure
Radiographic analysis has been efficient in identifying the main changes in the internal morphology of seeds, being increasingly used in quality control programs due to their practicality, objectivity and speed in obtaining the results. Despite these advantages, there are still no studies proving the efficiency of these techniques for coffee seed analysis. Thereby, the aim of this research was to evaluate the internal morphology of coffee seeds (Coffea arabica L.) through the analysis of radiographic images and their relation with germination performance. Radiographic images of seeds of the cultivars Bourbon and Catucaí 20/15, each represented by six lots, were examined and the seeds were seeded and seedling emergence, shoot length, stem diameter and shoot dry mass were evaluated. Seeds of Bourbon presented high percentage of intact seeds, as well as better performance in the germination of the lots compared to Catucaí 20/15, which presented more incidence of damaged seeds and lower germination. The radiographic images allowed identifying seeds with malformations, tissue deterioration and damage caused by the coffee borer. This non-destructive imaging technique is efficient in identifying intact seeds, with potential to germinate and produce vigorus seedlings.
Water stress in germination, growth and development of coffee cultivars
(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2018) Almeida, Julieta Andréa Silva de; Azevedo, Mary Túlia Vargas Lobato Guedes de; Salomon, Marcus Vinicius; Medina, Priscila Fratin
The aim of this study was to evaluate the effect of water stress on the germination of Coffea arabica seeds and the growth and development of plants from these seeds. Seeds of the cultivars Bourbon Vermelho, Mundo Novo, and BA-10 were placed to germinate in the dark at 30 ºC in the presence and absence of 100 g/L of polyethylene glycol 6000. The water stress applied delayed the beginning of seed germination by seven days in relation to seeds of the control, which had achieved responses of up to 50%, but there were no differences between the treatments at the end of the experiment. Furthermore, the length of the primary root in seeds germinated under water stress was less than in the control treatment, especially for the cultivar Mundo Novo. Plants of the cultivars Bourbon Vermelho and Mundo Novo developed from seeds previously germinated under water stress exhibited reduced height and fewer leaf pairs than those from the control, whereas ‘BA-10’ responded in a similar way in both treatments. Thus, the cultivars Bourbon Vermelho and Mundo Novo were more sensitive to the water stress applied in the germination phase than ‘BA-10’.