Journal of Seed Science

URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/13100

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2013 - 20193

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Data final: 2019Assunto: search.filters.subject.Coffea arabica

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Agora exibindo 1 - 3 de 3
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    Minimum period to assess the potential of germination of coffee seeds
    (Associação Brasileira de Tecnologia de Sementes - ABRATES, 2013) Guimarães, Gabriel Castanheira; Rosa, Sttela Dellyzette Veiga Franco da; Coelho, Luis Filipe Serafim; Veiga, Adriano Delly; Clemente, Aline da Consolação Sampaio
    In recent research, early stages of coffee seedling development were identified, after radicle protrusion, in which all the essential parts of the seedling can be assessed to demonstrate the potential of seed germination to generate normal plants. Thus, the objective of this research was to develop studies to reduce the time on the germination test. Ten seed lots of coffee were assessed through the germination test, every two days, from the tenth day of sowing, according to the morphological criteria of the seedlings. The data were compared to the standard germination test and analyzed by means of regression analysis and segmented nonlinear regression and planteau response model. It was concluded that the assessment of coffee seedlings at 16 days, in stage S1, provides the similar result of the pattern germination test, allowing a significant reduction of time in order to demonstrate the germination of the seed lots; the seedling assessment coffee in stage S2 does not have reduction of the time for assessing the coffee germination, in relation to the pattern.
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    Water stress in germination, growth and development of coffee cultivars
    (Associação Brasileira de Tecnologia de Sementes - ABRATES, 2018) Almeida, Julieta Andréa Silva de; Azevedo, Mary Túlia Vargas Lobato Guedes de; Salomon, Marcus Vinicius; Medina, Priscila Fratin
    The aim of this study was to evaluate the effect of water stress on the germination of Coffea arabica seeds and the growth and development of plants from these seeds. Seeds of the cultivars Bourbon Vermelho, Mundo Novo, and BA-10 were placed to germinate in the dark at 30 ºC in the presence and absence of 100 g/L of polyethylene glycol 6000. The water stress applied delayed the beginning of seed germination by seven days in relation to seeds of the control, which had achieved responses of up to 50%, but there were no differences between the treatments at the end of the experiment. Furthermore, the length of the primary root in seeds germinated under water stress was less than in the control treatment, especially for the cultivar Mundo Novo. Plants of the cultivars Bourbon Vermelho and Mundo Novo developed from seeds previously germinated under water stress exhibited reduced height and fewer leaf pairs than those from the control, whereas ‘BA-10’ responded in a similar way in both treatments. Thus, the cultivars Bourbon Vermelho and Mundo Novo were more sensitive to the water stress applied in the germination phase than ‘BA-10’.
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    Gene expression during the germination of coffee seed
    (Associação Brasileira de Tecnologia de Sementes - ABRATES, 2019) Silva, Edvaldo Aparecido Amaral da; Acencio, Marcio Luis; Bovolenta, Luiz Augusto; Lemke, Ney; Varani, Alessandro de Mello; Bravo, Juliana Pereira; Hoshino-Bezerra, Andrea Akemi; Lemos, Eliana Gertrudes Macedo
    Germination of the coffee (Coffea arabica L.) seed is the result of events that occur simultaneously in the embryo and endosperm. To understand the molecular mechanisms responsible for these events, we undertook a transcriptome analysis of embryo, micropylar and lateral endosperms from 10-day-imbibed seeds. The sequencing yielded contigs coding for 16,813 proteins. From those, 14,005 (~ 83%) were highly similar to at least one protein sequence in the nr database. 162 genes were significantly expressed in the embryo, 36 in the micropylar endosperm and 72 in the lateral endosperm. The tissue specificity analysis of the significantly expressed genes showed that the embryo had the highest proportion of specific genes (113/162, ~70%), while 11 were expressed in the micropylar and lateral endosperms. In the embryo, genes were mainly associated with abiotic stress, cell growth, and intercellular communication. In the micropylar and lateral endosperms, they were associated with abiotic stress and cell wall degradation. The accuracy of RNA-seq data was confirmed by RT-qPCR. This work adds new information about the molecular mechanism involved in coffee seed germination.