Crop Breeding and Applied Biotechnology

URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/12091

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    A new set of quantitative trait loci linked to lipid content in Coffea arabica
    (Crop Breeding and Applied Biotechnology, 2024-04-10) Muniz, Herison Victor Lima; Ariyoshi, Caroline; Ferreira, Rafaelle Vecchia; Felicio, Mariane Silva; Pereira, Luiz Filipe Protasio
    Lipids are compounds that play an important role in coffee bean development, contributing to beverage quality. Genome-wide association studies (GWAS) were conducted to pinpoint quantitative trait nucleotides (QTNs) linked to lipid metabolism in Coffea arabica. Genotyping by sequencing (GBS) and phenotyping data from 104 wild C. arabica accessions, Mundo Novo cultivar, and C. arabica var. Typica were utilized. GBS data were aligned to C. arabica Et039 reference genome, and both single-locus and multi-locus GWAS methods were employed. Methods were adjusted for kinship matrix, population structure, and principal component analysis. Of the 19 QTNs identified, 5 showed consistency across different population structure adjustments. The multi-locus methods mrMLM and FarmCPU proved more effective in identifying QTNs associated with lipid content. Four QTNs were situated near seven genes potentially involved in lipid metabolism. Higher frequencies of identified QTNs in accessions with elevated lipid content suggest their utility as markers for coffee plant breeding.
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    Single-locus inheritance and partial linkage map of Coffea arabica L.
    (Crop Breeding and Applied Biotechnology, 2004) Sakiyama, Ney Sussumu; Teixeira-Cabral, Terezinha Aparecida; Zambolim, Laércio; Pereira, Antonio Alves; Schuster, Ivan
    In a backcross population of the allotetraploid Coffea arabica L. the loci with diploid-like segregation were predominant, although a few loci with tetrassomic inheritance or distortion of the expected segregation were also observed. A partial genetic map of Coffea arabica L. was constructed with 82 RAPD loci scored in this backcross population of 104 individuals. It covered the estimated length of 540.6 cM in eight linkage groups. The linkage group size was highly correlated with the number of markers, indicating random distribution of the markers in the groups. The average distance between two markers was 7.3 cM.