Crop Breeding and Applied Biotechnology

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Large-scale, high-efficiency production of coffee somatic embryos
(Crop Breeding and Applied Biotechnology, 2016-03) Maciel, Anna Lygia de Rezende; Rodrigues, Filipe Almendagna; Pasqual, Moacir; Carvalho, Carlos Henrique Siqueira de
The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immer- sion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L -1 ) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L -1 ) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L -1 BA and 0.0 mg L -1 IAA yielded the highest percentage of large cotyledonary embryos.