Crop Breeding and Applied Biotechnology
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/12091
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Resultados da Pesquisa
Item Single-locus inheritance and partial linkage map of Coffea arabica L.(Crop Breeding and Applied Biotechnology, 2004) Sakiyama, Ney Sussumu; Teixeira-Cabral, Terezinha Aparecida; Zambolim, Laércio; Pereira, Antonio Alves; Schuster, IvanIn a backcross population of the allotetraploid Coffea arabica L. the loci with diploid-like segregation were predominant, although a few loci with tetrassomic inheritance or distortion of the expected segregation were also observed. A partial genetic map of Coffea arabica L. was constructed with 82 RAPD loci scored in this backcross population of 104 individuals. It covered the estimated length of 540.6 cM in eight linkage groups. The linkage group size was highly correlated with the number of markers, indicating random distribution of the markers in the groups. The average distance between two markers was 7.3 cM.Item Repeatability and number of harvests required for selection in robusta coffee(Crop Breeding and Applied Biotechnology, 2004) Fonseca, Aymbiré Francisco Almeida da; Sediyama, Tocio; Cruz, Cosme Damião; Sakiyama, Ney Sussumu; Ferrão, Romário Gava; Ferrão, Maria Amélia Gava; Bragança, Scheilla MarinaThis study aimed to estimate the repeatability coefficient of the grain yield in Coffea canephora by three methods: to quantify the precision of the measurements; to predict the real value of an individual based on n evaluations; and to determine the number of phenotypic measures required in each plant to obtain an adequate precision level for an efficient discrimination of the genotypes. The coefficients of repeatability and determination were estimated based on four harvests of 80 genotypes. Highest estimates of the repeatability coefficient were obtained by the method of the principal components derived from the matrix of covariances, which expresses the correlation between each measurement pair. The prediction precision of the real individual value ranged from 65.32 to 81.59%, and remained practically unchanged from the sixth harvest on.Item Reproducibility of the RAPD marker and its efficiency in coffee tree genotype grouping analysis(Crop Breeding and Applied Biotechnology, 2002) Sakiyama, Ney Sussumu; Teixeira-Cabral, Terezinha Aparecida; Zambolim, Laércio; Pereira, Antonio Alves; Barros, Everaldo Gonçalves; Sakiyama, Cássia Camargo HargerThe genetic diversity of Coffea arabic L. cultivars is relatively narrow and its assessment and increase is important for breeding. Fifty two arbitrary primers were used to evaluate the reproducibility and the influence of the number of RAPD (Random Amplified Polymorphic DNA) markers on the estimation of genetic distances among 40 genotypes of Coffea spp. The average number of polymorphic bands was 6.69 per primer among all genotypes, and 1.27 among arabica coffee genotypes. RAPD markers were efficient in estimating the genetic distances among the genotypes. The increase in RAPD loci number during grouping analysis did not affect the major groups’ composition; however, it affected the composition of subgroups. Marker reproducibility was 76.88% and replicated data was recommended for distinguishing genotypes with the same genetic background.Item Polymorphic information content of SSR markers for Coffea spp.(Crop Breeding and Applied Biotechnology, 2010) Caixeta, Eveline Teixera; Missio, Robson Fernando; Zambolim, Eunize Maciel; Zambolim, Laércio; Cruz, Cosme Damião; Sakiyama, Ney SussumuThirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzed in 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to 13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values were found for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphic SSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and the leaf rust resistant arabica cultivars.Item Grafted young coffee tree growth in a greenhouse(Crop Breeding and Applied Biotechnology, 2002) Sakiyama, Ney Sussumu; Tomaz, Marcelo Antonio; Martinez, Hermínia Emília Pietro; Pereira, Antonio Alves; Zambolim, Laércio; Cruz, Cosme DamiãoGrafted young coffee trees were observed in a greenhouse to study the effect of different scions and rootstocks on plant growth. Four Coffea arabica L. genotypes were used as scions: the cultivars Catuaí Vermelho IAC 15 and Oeiras MG 6851, and the progenies H 419-10-3-1-5 and H 514-5-5-3. They were also used as nongrafted control plants. Four genotypes were used as rootstocks: ‘Apoatã IAC 2258’ (C. canephora), ‘Conillon’ (C. canephora), ‘Emcapa 8141’ (C. canephora), and ‘Mundo Novo IAC 376-4’ (C. arabica). ‘Mundo Novo IAC 376-4’ and ‘Apoatã IAC 2258’ were classified as good rootstocks, while ‘Oeiras MG 6851’ and “H 419- 10-3-1-5” performed well as non-grafted plants. The diallel analysis statistical model was efficient to evaluate the general combination ability of the rootstocks and, therefore, recommended for rootstock selection procedures in breeding programs.Item Characterization of differential coffee tree hostsfor Hemileia vastatrix Berk. et Br. with RAPD markers(Crop Breeding and Applied Biotechnology, 2004-03-12) Teixeira-Cabral, Terezinha Aparecida; Sakiyama, Ney Sussumu; Zambolim, Laércio; Barros, Everaldo Gonçalves de; Silva, Dalza Gomes daEighteen clones of differential coffee tree hosts for Hemileia vastatrix Berk. et Br. were characterized with RAPD markers. The genetic distances were estimated and the genealogical origin of the clones compared to data of markerbased clusters. Thirty-five primers identified 158 polymorphic loci of RAPD markers. The cluster based on the matrix of genetic dissimilarity values was compatible with information on the genealogical origin cited in literature. Specific markers for a number of clones were identified, and a combination of 12 RAPD markers allowed the characterization of the studied clones.Item Discriminant analysis for the classification and clustering of robusta coffee genotypes(Crop Breeding and Applied Biotechnology, 2004-07-07) Fonseca, Aymbiré Francisco Almeida da; Sediyama, Tocio; Cruz, Cosme Damião; Sakiyama, Ney Sussumu; Ferrão, Romário Gava; Ferrão, Maria Amélia Gava; Bragança, Scheilla MarinaThis study evaluated the adequacy of the composition of three clonal Coffea canephora varieties recommended for the State of Espírito Santo by a multivariate method designated discriminant analysis. This method consists in the establishment of functions that enable the classification of a given individual into one, among various distinct populations, reducing the probability of a misclassification. It simultaneously considers measures of several traits, in order to give the new variety homogeneity. The original classification of genotypes in the three studied varieties, based on agronomical criteria, maintained expressive concordance with the results of the discriminant analysis, with an apparent deviation rate of only 6.25%. Corrected discriminant functions were also proposed, capable of classifying a new genotype into one of the three clonal varieties to be used in improvement programs, eliminating the subjectivity of the clustering process.Item Partial map of Coffea arabica L. and recovery of the recurrent parent in backcross progenies(Crop Breeding and Applied Biotechnology, 2007-06-30) Oliveira, Antonio Carlos Baião de; Sakiyama, Ney Sussumu; Caixeta, Eveline Teixeira; Zambolim, Eunize Maciel; Rufino, Raphael José Nascif; Zambolim, LaércioA partial map of Coffea arabica L. was constructed based on a backcross population and RAPD markers. From a total of 178 markers evaluated, only 134 that segregated 1:1 (P>0.05) were used to develop the map. Seventeen markers were not linked, while 117 formed 11 linkage groups, covering a genome distance of 803.2 cM. The maximum distance between adjacent markers was 26.9 cM, and only seven intervals exceeded 20 cM. The markers were further used for assisted selection of the plants closest to the recurrent parent, to accelerate the introgression of rust resistance genes in the coffee breeding program. Three BC1 plants resistant to coffee leaf rust and with high genetic similarity to ?Catuaí? were selected and integrated in the following backcross cycles.Item Triple plagiotropic branch in coffee: a new promising mutant?(Crop Breeding and Applied Biotechnology, 2017-10) Sakiyama, Ney Sussumu; Picoli, Edgard Augusto de Toledo; Oliveira, Antonio Carlos Baião de; Caixeta, Eveline Teixeira; Zambolim, Laércio; Martinez, Hermínia Emília Prieto; Pereira, Antonio AlvesCoffee tree is a shrub with dimorphic branches. The orthotropic stem grows vertically with two near-horizontal plagiotropic branches, per node, in opposite phyllotaxy. The consecutive nodes of the orthotropic stem are organ- ized in parastichies. We report here mutant plants of Coffea arabica with triple plagiotropic branch, resulted from the whorled phyllotaxy of the orthotropic stem. In mutant plants three plagiotropic branches in the same node are sepa- rated by angles of approximately 120 o , while in normal plants two plagiotropic branches in the same node are separated by angles of approximately 180 o . In mutant plants the angles of the parastichies increase approximately 50 o clock- wise between consecutive nodes, while in normal plants the angles increase approximately 87 o . The plant architecture is changed. The triple plagiotropic branch is a putative mutant with potential to be explored in coffee breeding programs. The hypotheses for the origin of the mutation and the research perspectives are discussed.Item Molecular diversity in Coffea canephora germplasm conserved and cultivated in Brazil(Crop Breeding and Applied Biotechnology, 2013-12) Souza, Flávio de França; Caixeta, Eveline Teixeira; Ferrão, Luís Felipe Ventorim; Pena, Guilherme Ferreira; Sakiyama, Ney Sussumu; Zambolim, Eunize Maciel; Zambolim, Laércio; Cruz, Cosme DamiãoThis work aimed to characterize accessions that represent the C. canephora germplasm conserved and cultivated in Brazil. A total of 130 accessions from germplasm banks of IAC (São Paulo), UFV (Minas Gerais) and also collected in plantations of the State of Espírito Santo and Rondônia were evaluated with a set of 20 new microsatellite primers. Multivariate methods were used to estimate the relationship among the accessions. High level of polymorphism and two major diversity clusters were identified. First cluster was composed by the accessions conserved in the IAC and UFV collections and the second was formed by accessions collected in areas under cultivation. Accessions from Espírito Santo and Rondônia were clear separated, composing two subclusters. Despite the great polymorphism found in Brazilian plantations, the diversity may be increased, because a new threshold in the genetic gains is expected on breeding programs with the intensification of the use of conserved germplasm