Crop Breeding and Applied Biotechnology
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/12091
Navegar
15 resultados
Resultados da Pesquisa
Item IAC Herculândia - a Coffea canephora rootstock multiresistant to Meloidogyne species(Crop Breeding and Applied Biotechnology, 2023-08-20) Guerreiro Filho, Oliveiro; Fazuoli, Luiz Carlos; Braghini, Masako Toma; Gallo, Paulo Boller; Oliveira, Claúdio Marcelo Gonçalves; Padilha, Lilian; Fatobene, Barbhara Joana dos Reis; Andrade, Vinicius Teixeira; Vasconcelos, Larissa de Brito Caixeta; Rosa, Juliana Magrinelli OsórioIAC Herculândia is a Coffea canephora cultivar multiresistant to Meloidogyne exigua, M. incognita, and M. paranaensis to be used as a rootstock for Coffea arabica cultivars. It is a synthetic cultivar resulting from recombination among the clonal cultivars IAC WG, IAC FEBS, IAC PM, IAC LCCBF, and IAC ARM.Item MGS Guaiçara and MGS Vereda: Coffea arabica cultivars resistant to the root-knot nematode Meloidogyne paranaensis(Crop Breeding and Applied Biotechnology, 2022-09-01) Salgado, Sônia Maria de Lima; Fatobene, Bárbhara Joana dos Reis; Pereira, Antonio Alves; Abrahão, Juliana Costa de Rezende; Botelho, Cesar Elias; Carvalho, Gladyston Rodrigues; Oliveira, Antônio Carlos Baião de; Ferreira, André Dominghetti; Figueiredo, Vanessa Castro; Vilela, Diego Junior Martins; Luz, Silvana Ramlow Otto Teixeira da; Andrade, Vinicius TeixeiraMGS Guaiçara e MGS Vereda are resistant to Meloidogyne paranaensis, one of the most aggressive nematode parasites of coffee. MGS Vereda is an early maturation cultivar, whereas MGS Guaiçara has a medium maturation cycle. Both cultivars produce red fruit, have high size, and high yield potential in infested areas.Item Characterization and genetic diversity of Coffea canephora accessions in a germplasm bank in Espírito Santo, Brazil(Crop Breeding and Applied Biotechnology, 2021-06-20) Ferrão, Maria Amélia Gava; Mendonça, Rodolfo Ferreira de; Fonseca, Aymbiré Francisco Almeida; Ferrão, Romário Gava; Senra, João Felipe Brites; Volpi, Paulo Sérgio; Verdin Filho, Abraão Carlos; Comério, MarconeThe state of Espírito Santo is the major producer of Coffea canephora in Brazil. Knowledge of genetic reserves is fundamental to plant breeding. Therefore, the present study characterized and analyzed the genetic diversity of 600 C. canephora accessions from the germplasm bank of Incaper based on 38 traits evaluated in 24-30-month-old plants. Further, the predominant descriptors or traits were identified, and high phenotypic variability was determined. Genetic distances for the grouped (Gower), quantitative, and qualitative datasets were 0.48, 0.61, and 0.92, respectively, with accessions 76 (Conilon) and 407 (Robusta) being the most divergent ones at Incaper. In clustering using the Tocher optimization method, 30 groups were formed, with three accessions introduced from Epamig’s Robusta collection being the most dissimilar ones. Graphical dispersion analysis using the principal coordinate method revealed the predominance of three groups formed by the Robusta, Conilon, and hybrid Robusta × Conilon genotypes.Item In vitro pollination and fluorescence microscopy for characterization of gametophytic selfincompatibility of Coffea canephora Pierre ex A. Froehner(Crop Breeding and Applied Biotechnology, 2021-11-20) Souza, Carolina Augusto de; Rocha, Rodrigo Barros; Santos, Maurício Reginaldo Alves dos; Lopes, Tatiane Almeida; Teixeira, Alexsandro Lara; Espindula, Marcelo CuritibaThe objectives of this study were to optimize in vitro pollination and fluorescence microscopy procedures to characterize the gametophytic selfincompatibility of Coffea canephora genotypes. For that purpose, 115 in vitro pollinations were carried out among 30 parent plants of the Conilon (17) and Robusta (13) botanical varieties. Tester plants of compatibility groups I, II, and III were used as pollen grain donors. On the day of anthesis, pollen grains of the donor genotypes were transferred in vitro to one of the sides of the bifid stigma of each receptor flower. After 30 hours, the development of the pollen tubes was evaluated in fluorescence microscopy. Eleven genotypes (37%) were classified in compatibility Group I, thirteen (43%) in Group II, and six (20%) in Group III. In vitro pollination allowed reduction in the time necessary to diagnose compatibility, without exhibiting contamination caused by the presence of pollen grains of non-donor plants.Item IAC Herculândia – a Coffea canephora rootstock multiresistant to Meloidogyne species(Crop Breeding and Applied Biotechnology, 2023-08-20) Guerreiro Filho, Oliveiro; Fazuoli, Luiz Carlos; Braghini, Masako Toma; Gallo, Paulo Boller; Oliveira, Claúdio Marcelo Gonçalves; Padilha, Lilian; Fatobene, Bárbhara Joana dos Reis; Andrade, Vinicius Teixeira; Vasconcelos, Larissa de Brito Caixeta; Rosa, Juliana Magrinelli OsórioIAC Herculândia is a Coffea canephora cultivar multiresistant to Meloidogyne exigua, M. incognita, and M. paranaensis to be used as a rootstock for Coffea arabica cultivars. It is a synthetic cultivar resulting from recombination among the clonal cultivars IAC WG, IAC FEBS, IAC PM, IAC LCCBF, and IAC ARM.Item MGS Guaiçara and MGS Vereda: Coffea arabica cultivars resistant to the root-knot nematode Meloidogyne paranaensis(Crop Breeding and Applied Biotechnology, 2022-09-01) Abrahão, Juliana Costa de Rezende; Salgado, Sônia Maria de Lima; Fatobene, Bárbhara Joana dos Reis; Pereira, Antonio Alves; Botelho, Cesar Elias; Carvalho, Gladyston Rodrigues; Oliveira, Antônio Carlos Baião de; Ferreira, André Dominghetti; Figueiredo, Vanessa Castro; Vilela, Diego Junior Martins; Luz, Silvana Ramlow Otto Teixeira da; Andrade, Vinicius TeixeiraMGS Guaiçara e MGS Vereda are resistant to Meloidogyne paranaensis, one of the most aggressive nematode parasites of coffee. MGS Vereda is an early maturation cultivar, whereas MGS Guaiçara has a medium maturation cycle. Both cultivars produce red fruit, have high size, and high yield potential in infested areas.Item Assisted selection using molecular markers linked to rust resistance SH3 gene in Coffea arabica(Crop Breeding and Applied Biotechnology, 2023-10-25) Silva, Angelita Garbossi; Ariyoshi, Caroline; Shigueoka, Luciana Harumi; Pereira, Luiz Filipe Protasio; Sera, Gustavo HiroshiThe aim of this work was to validate markers linked to the SH3 gene of coffee leaf rust (CLR) resistance and use them in assisted selection. Initially, we validated the markers in genotypes already known to carry SH3. Next, we performed phenotype and genotype evaluation for resistance to CLR in coffee plants growing under field conditions. We used Arabica coffee progenies derived from BA-10, which carries the SH3 gene due to introgression of C. liberica. Three SCAR markers (SP-M16-SH3, BA-48-21O-f, and BA-124-12K-f) and one SSR marker (Sat244) linked to SH3 gene were used to amplify the coffee plants’ DNA. Our assessments of markers validation in resistant genotypes, SP-M16-SH3 and BA-124-12K-f, were efficient to identify the SH3 gene. These two markers were used to evaluate the progenies derived from BA-10 and were significantly linked to the phenotype evaluations. The SP-M16-SH3 marker was more efficient, with the advantage of being codominant.Item A new set of quantitative trait loci linked to lipid content in Coffea arabica(Crop Breeding and Applied Biotechnology, 2024-04-10) Muniz, Herison Victor Lima; Ariyoshi, Caroline; Ferreira, Rafaelle Vecchia; Felicio, Mariane Silva; Pereira, Luiz Filipe ProtasioLipids are compounds that play an important role in coffee bean development, contributing to beverage quality. Genome-wide association studies (GWAS) were conducted to pinpoint quantitative trait nucleotides (QTNs) linked to lipid metabolism in Coffea arabica. Genotyping by sequencing (GBS) and phenotyping data from 104 wild C. arabica accessions, Mundo Novo cultivar, and C. arabica var. Typica were utilized. GBS data were aligned to C. arabica Et039 reference genome, and both single-locus and multi-locus GWAS methods were employed. Methods were adjusted for kinship matrix, population structure, and principal component analysis. Of the 19 QTNs identified, 5 showed consistency across different population structure adjustments. The multi-locus methods mrMLM and FarmCPU proved more effective in identifying QTNs associated with lipid content. Four QTNs were situated near seven genes potentially involved in lipid metabolism. Higher frequencies of identified QTNs in accessions with elevated lipid content suggest their utility as markers for coffee plant breeding.Item MGS Guaiçara and MGS Vereda: coffea arabica cultivars resistant to the root-knot nematode Meloidogyne paranaensis(Crop Breeding and Applied Biotechnology, 2022-09-19) Salgado, Sônia Maria de Lima; Fatobene, Bárbhara Joana dos Reis; Pereira, Antonio Alves; Abrahão, Juliana Costa de Rezende; Botelho, Cesar Elias; Carvalho, Gladyston Rodrigues; Oliveira, Antônio Carlos Baião de; Ferreira, André Dominghetti; Figueiredo, Vanessa Castro; Vilela, Diego Junior Martins; Luz, Silvana Ramlow Otto Teixeira da; Andrade, Vinicius TeixeiraMGS Guaiçara e MGS Vereda are resistant to Meloidogyne paranaensis, one of the most aggressive nematode parasites of coffee. MGS Vereda is an early maturation cultivar, whereas MGS Guaiçara has a medium maturation cycle. Both cultivars produce red fruit, have high size, and high yield potential in infested areas.Item Characterization and genetic diversity of Coffea canephora accessions in a germplasm bank in Espírito Santo, Brazil(Crop Breeding and Applied Biotechnology, 2021) Ferrão, Maria Amélia Gava; Mendonça, Rodolfo Ferreira de; Fonseca, Aymbiré Francisco Almeida; Ferrão, Romário Gava; Senra, João Felipe Brites; Volpi, Paulo Sérgio; Verdin Filho, Abraão Carlos; Comério, MarconeThe state of Espírito Santo is the major producer of Coffea canephora in Brazil. Knowledge of genetic reserves is fundamental to plant breeding. Therefore, the present study characterized and analyzed the genetic diversity of 600 C. canephora accessions from the germplasm bank of Incaper based on 38 traits evaluated in 24-30-month-old plants. Further, the predominant descriptors or traits were identified, and high phenotypic variability was determined. Genetic distances for the grouped (Gower), quantitative, and qualitative datasets were 0.48, 0.61, and 0.92, respectively, with accessions 76 (Conilon) and 407 (Robusta) being the most divergent ones at Incaper. In clustering using the Tocher optimization method, 30 groups were formed, with three accessions introduced from Epamig’s Robusta collection being the most dissimilar ones. Graphical dispersion analysis using the principal coordinate method revealed the predominance of three groups formed by the Robusta, Conilon, and hybrid Robusta × Conilon genotypes.