Biblioteca do Café
URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/1
Navegar
2 resultados
Resultados da Pesquisa
Item Geostatistical analysis of bacterial blight in coffee tree seedlings in the nursery(Grupo Paulista de Fitopatologia, 2018) Belan, Leônidas Leoni; Pozza, Edson Ampélio; Alves, Marcelo de Carvalho; Freitas, Marcelo Loran de OliveiraIn nurseries of coffee tree seedlings (Coffea arabica), there are favorable conditions for bacterial blight epidemics (Pseudomonas syringae pv. garcae). Studying the spatial distribution of diseased plants can help in the adoption of management strategies. Likewise, geostatistics has been applied to shape the spatial distribution and study epidemiological aspects of plant diseases. Thus, this study was developed to characterize the spatial distribution pattern of bacterial blight in a nursery of coffee tree seedlings. The disease progress was monitored over time in 704 seedlings organized in lines and columns in a nursery. Considering the mean diameter of the pots used for producing seedlings, georeferencing was carried out in Cartesian coordinate system for the seedlings in the nursery. The disease incidence data were subjected to non-spatial exploratory analysis and geostatistical analysis. The spherical isotropic semivariogram model was adjusted to the data and data interpolation was performed by ordinary kriging to visualize the spatial distribution of symptomatic seedlings. Bacterial blight epidemic was detected in the nursery during the experimental period, and there was variability and spatial dependence in relation to the distribution of diseased seedlings. As the epidemic progressed, the population of diseased plants increased, as well as the number and the size of the foci and their coalescence. Besides, there was an increase in the range value, sill and nugget effect. The kriging maps showed the disease progress and its variance. The bacterial blight epidemic of coffee tree started with a random spatial distribution pattern, progressing to an aggregate pattern.Item Initial stages of infectious process of Pseudomonas syringae pv. garcae in coffee leaves(Editora UFLA, 2019-07) Souza, José Otávio Gusmão de; Pozza, Edson Ampélio; Belan, Leônidas Leoni; Freitas, Marcelo Loran de Oliveira; Oliveira, Júlia MarquesBacterial blight of coffee (Pseudomonas syringae pv. garcae) (PSG) has been causing damage and losses to coffee growing. However, initial stages of the infectious process should be described in order to understand the relationship cycle and to propose efficient management strategies. This study described the initial stages of infectious process of the PSG in coffee leaves over time. The bacterial penetration sites studied were stomata, leaf margins, and wounds. Coffee seedlings received 50μL inoculum suspension at marked points on the abaxial face and leaf margins, and around wounds. By 30 minutes, 1, 3, 6, and 12 hours after inoculation, leaf disks collected at inoculated points were analyzed with scanning electron microscopy. One hour after inoculation, bacterial groups could be seen as result of cell multiplication. Subsequently, the number of grouped bacteria increased proportionally over time. A growing population concentration was found near stomata, wounds, epidermal appendages, and wax layer on leaf margins and leaf tip. From these places bacteria were positioned in their long axis starting from structures, thus created a population gradient and displacement flow toward penetration sites. Initial stages of infectious process occurred in all penetration sites, thus it was not possible to determine a preferred pathway.