Biblioteca do Café

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Molecular identification of Aspergillus spp. isolated from coffee beans
(Escola Superior de Agricultura "Luiz de Queiroz", 2005-01) Magnani, Marciane; Fernandes, Thiago; Prete, Cássio Egidio Cavenaghi; Homechim, Martin; Ono, Elisabete Yurie Sataque; Vilas-Boas, Laurival Antonio; Sartori, Daniele; Furlaneto, Márcia Cristina; Fungaro, Maria Helena Pelegrinelli
Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) of ribosomal DNA (rDNA). The results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which Aspergillus niger was the species most frequently detected, followed by A. ochraceus and A. carbonarius. The presence of A. carbonarius in immature coffee fruits harvested from trees is reported for the first time.