Biblioteca do Café
URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/1
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Resultados da Pesquisa
Item Stomatal behavior and components of the antioxidative system in coffee plants under water stress(Escola Superior de Agricultura "Luiz de Queiroz", 2011-01) Deuner, Sidnei; Alves, José Donizeti; Zanandrea, Ilisandra; Goulart, Patrícia de Fátima Pereira; Silveira, Neidiquele Maria; Henrique, Paôla de Castro; Mesquita, Alessandro CarlosCoffee (Coffea arabica) plants show a positive relationship between stomatal closure and formation and accumulation of H2O2. However, for coffee plants under water restriction such relationship has never been studied. The objective of the present study was evaluate the stomatal movement and the antioxidant capacity of coffee seedlings under different water regimes. Eight months old coffee seedlings of cv. Catuaí IAC 99 were submitted to field capacity, gradual and total suspension of irrigation during a period of 21 days. Evaluations of leaf water potential (Ψ w) were performed in the beginning of the morning, and stomatal resistance, transpiration rate and vapor pressure deficit were determined at 10 am and 5 pm. All biochemical and enzymatic determinations were performed in leaves collected at 5 pm. Evaluations and samplings were performed at three days intervals. There was no variation in Ψ w during the evaluated period for plants in field capacity. However, an expressive decrease of Ψ w following day 12, reaching values near -2.5 MPa at the end of the experiment was observed for plants submitted to gradual suspension of irrigation. For plants submitted to total suspension of irrigation, Ψ w decreases after the sixth day, reaching -2.5 MPa at day 15. The decay of Ψ w in plants submitted to gradual and total suspension of irrigation reflected in increased stomatal resistance and in a decreased transpiration rate leading to an increase in hydrogen peroxide formation and, on final stages, increase in lipid peroxidation. As a conclusion, an increase in the activity of antioxidant enzymes as well as in the levels of ascorbate and dehydroascorbate was observed, which act in the detoxification of free radicals formed as result of the water stress.Item Sistema antioxidante de cafeeiros cultivados em diferentes altitudes na Serra da Mantiqueira(Embrapa Café, 2015) Silveira, Helbert Rezende de Oliveira; Souza, Kamila Rezende Dázio de; Brandão, Isabel Rodrigues; Santos, Meline de Oliveira; Goulart, Patrícia de Fátima Pereira; Alves, Jose DonizetiO objetivo deste trabalho foi avaliar os níveis de ascorbato e peróxido de hidrogênio, bem como a peroxidação lipídica em cafeeiros situados em três diferentes altitudes da região da Serra da Mantiqueira. Foram realizadas avaliações nas quatro estações do ano, em que coletou-se folhas completamente expandidas do terço mediano das plantas. Avaliou-se a geração de peróxido de hidrogênio, níveis de peroxidação lipídica e concentração de ascorbato. Os cafeeiros da maior altitude apresentaram, em todas as estações do ano, maiores concentrações de ascorbato, menor geração de peróxido de hidrogênio e menores níveis de peroxidação lipídica do que os cafeeiros da menor altitude. Os atributos ligados à maior qualidade da bebida de cafés de altitudes mais elevadas podem também estar relacionados com a maior atuação, na planta, do sistema antioxidante para neutralização das espécies reativas de oxigênio, reduzindo os danos às membranas celulares.