Biblioteca do Café
URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/1
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Item Single-locus inheritance and partial linkage map of Coffea arabica L.(Crop Breeding and Applied Biotechnology, 2004) Sakiyama, Ney Sussumu; Teixeira-Cabral, Terezinha Aparecida; Zambolim, Laércio; Pereira, Antonio Alves; Schuster, IvanIn a backcross population of the allotetraploid Coffea arabica L. the loci with diploid-like segregation were predominant, although a few loci with tetrassomic inheritance or distortion of the expected segregation were also observed. A partial genetic map of Coffea arabica L. was constructed with 82 RAPD loci scored in this backcross population of 104 individuals. It covered the estimated length of 540.6 cM in eight linkage groups. The linkage group size was highly correlated with the number of markers, indicating random distribution of the markers in the groups. The average distance between two markers was 7.3 cM.Item Repeatability and number of harvests required for selection in robusta coffee(Crop Breeding and Applied Biotechnology, 2004) Fonseca, Aymbiré Francisco Almeida da; Sediyama, Tocio; Cruz, Cosme Damião; Sakiyama, Ney Sussumu; Ferrão, Romário Gava; Ferrão, Maria Amélia Gava; Bragança, Scheilla MarinaThis study aimed to estimate the repeatability coefficient of the grain yield in Coffea canephora by three methods: to quantify the precision of the measurements; to predict the real value of an individual based on n evaluations; and to determine the number of phenotypic measures required in each plant to obtain an adequate precision level for an efficient discrimination of the genotypes. The coefficients of repeatability and determination were estimated based on four harvests of 80 genotypes. Highest estimates of the repeatability coefficient were obtained by the method of the principal components derived from the matrix of covariances, which expresses the correlation between each measurement pair. The prediction precision of the real individual value ranged from 65.32 to 81.59%, and remained practically unchanged from the sixth harvest on.Item Reproducibility of the RAPD marker and its efficiency in coffee tree genotype grouping analysis(Crop Breeding and Applied Biotechnology, 2002) Sakiyama, Ney Sussumu; Teixeira-Cabral, Terezinha Aparecida; Zambolim, Laércio; Pereira, Antonio Alves; Barros, Everaldo Gonçalves; Sakiyama, Cássia Camargo HargerThe genetic diversity of Coffea arabic L. cultivars is relatively narrow and its assessment and increase is important for breeding. Fifty two arbitrary primers were used to evaluate the reproducibility and the influence of the number of RAPD (Random Amplified Polymorphic DNA) markers on the estimation of genetic distances among 40 genotypes of Coffea spp. The average number of polymorphic bands was 6.69 per primer among all genotypes, and 1.27 among arabica coffee genotypes. RAPD markers were efficient in estimating the genetic distances among the genotypes. The increase in RAPD loci number during grouping analysis did not affect the major groups’ composition; however, it affected the composition of subgroups. Marker reproducibility was 76.88% and replicated data was recommended for distinguishing genotypes with the same genetic background.Item Polymorphic information content of SSR markers for Coffea spp.(Crop Breeding and Applied Biotechnology, 2010) Caixeta, Eveline Teixera; Missio, Robson Fernando; Zambolim, Eunize Maciel; Zambolim, Laércio; Cruz, Cosme Damião; Sakiyama, Ney SussumuThirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzed in 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to 13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values were found for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphic SSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and the leaf rust resistant arabica cultivars.Item Grafted young coffee tree growth in a greenhouse(Crop Breeding and Applied Biotechnology, 2002) Sakiyama, Ney Sussumu; Tomaz, Marcelo Antonio; Martinez, Hermínia Emília Pietro; Pereira, Antonio Alves; Zambolim, Laércio; Cruz, Cosme DamiãoGrafted young coffee trees were observed in a greenhouse to study the effect of different scions and rootstocks on plant growth. Four Coffea arabica L. genotypes were used as scions: the cultivars Catuaí Vermelho IAC 15 and Oeiras MG 6851, and the progenies H 419-10-3-1-5 and H 514-5-5-3. They were also used as nongrafted control plants. Four genotypes were used as rootstocks: ‘Apoatã IAC 2258’ (C. canephora), ‘Conillon’ (C. canephora), ‘Emcapa 8141’ (C. canephora), and ‘Mundo Novo IAC 376-4’ (C. arabica). ‘Mundo Novo IAC 376-4’ and ‘Apoatã IAC 2258’ were classified as good rootstocks, while ‘Oeiras MG 6851’ and “H 419- 10-3-1-5” performed well as non-grafted plants. The diallel analysis statistical model was efficient to evaluate the general combination ability of the rootstocks and, therefore, recommended for rootstock selection procedures in breeding programs.Item Comparison of nutritional efficiency among hydroponic grafted young coffee trees for N, P, and K(Crop Breeding and Applied Biotechnology, 2004) Tomaz, Marcelo Antonio; Sakiyama, Ney Sussumu; Martinez, Hermínia Emília Prieto; Cruz, Cosme Damião; Zambolim, Laércio; Pereira, Antonio AlvesGrafted Coffea arabica L. plants were grown hydroponically in a greenhouse for an evaluation of their nutritional efficiency under the influence of different rootstocks. Four C. arabica genotypes were used as scions: ‘Catuaí Vermelho IAC 15’, ‘Oeiras MG 6851’, and lines H 419-10-3-1-5, and H 514-5-5-3. The three latter are cultivars resistant to the Hemileia vastatrix, pathogen of the orange rust. Rootstocks were three genotypes of Coffea canephora Pierre et Froenher: Apoatã LC 2258, Conilon, and Robustão Capixaba (Emcapa 8141), and one C. arabica genotype: Mundo Novo IAC 376-4. Rootstocks Apoatã LC 2258 and Mundo Novo IAC 376-4 were outstanding. Cultivars Conilon and Robustão Capixaba did not increase the nutritional efficiency of the scions. ‘Oeiras’ and H 419-10-3-1-5 had considerable nutrition efficiency when used as nongrafted plants.Item Characterization of differential coffee tree hostsfor Hemileia vastatrix Berk. et Br. with RAPD markers(Crop Breeding and Applied Biotechnology, 2004-03-12) Teixeira-Cabral, Terezinha Aparecida; Sakiyama, Ney Sussumu; Zambolim, Laércio; Barros, Everaldo Gonçalves de; Silva, Dalza Gomes daEighteen clones of differential coffee tree hosts for Hemileia vastatrix Berk. et Br. were characterized with RAPD markers. The genetic distances were estimated and the genealogical origin of the clones compared to data of markerbased clusters. Thirty-five primers identified 158 polymorphic loci of RAPD markers. The cluster based on the matrix of genetic dissimilarity values was compatible with information on the genealogical origin cited in literature. Specific markers for a number of clones were identified, and a combination of 12 RAPD markers allowed the characterization of the studied clones.Item Discriminant analysis for the classification and clustering of robusta coffee genotypes(Crop Breeding and Applied Biotechnology, 2004-07-07) Fonseca, Aymbiré Francisco Almeida da; Sediyama, Tocio; Cruz, Cosme Damião; Sakiyama, Ney Sussumu; Ferrão, Romário Gava; Ferrão, Maria Amélia Gava; Bragança, Scheilla MarinaThis study evaluated the adequacy of the composition of three clonal Coffea canephora varieties recommended for the State of Espírito Santo by a multivariate method designated discriminant analysis. This method consists in the establishment of functions that enable the classification of a given individual into one, among various distinct populations, reducing the probability of a misclassification. It simultaneously considers measures of several traits, in order to give the new variety homogeneity. The original classification of genotypes in the three studied varieties, based on agronomical criteria, maintained expressive concordance with the results of the discriminant analysis, with an apparent deviation rate of only 6.25%. Corrected discriminant functions were also proposed, capable of classifying a new genotype into one of the three clonal varieties to be used in improvement programs, eliminating the subjectivity of the clustering process.Item Partial map of Coffea arabica L. and recovery of the recurrent parent in backcross progenies(Crop Breeding and Applied Biotechnology, 2007-06-30) Oliveira, Antonio Carlos Baião de; Sakiyama, Ney Sussumu; Caixeta, Eveline Teixeira; Zambolim, Eunize Maciel; Rufino, Raphael José Nascif; Zambolim, LaércioA partial map of Coffea arabica L. was constructed based on a backcross population and RAPD markers. From a total of 178 markers evaluated, only 134 that segregated 1:1 (P>0.05) were used to develop the map. Seventeen markers were not linked, while 117 formed 11 linkage groups, covering a genome distance of 803.2 cM. The maximum distance between adjacent markers was 26.9 cM, and only seven intervals exceeded 20 cM. The markers were further used for assisted selection of the plants closest to the recurrent parent, to accelerate the introgression of rust resistance genes in the coffee breeding program. Three BC1 plants resistant to coffee leaf rust and with high genetic similarity to ?Catuaí? were selected and integrated in the following backcross cycles.Item Secafé: metodologia para acelerar a germinação das sementes de café(Associação Brasileira de Tecnologia de Sementes, 2007-07-02) Meireles, Robson Celestino; Araujo, Eduardo Fontes; Reis, Múcio Silva; Sediyama, Carlos Sigueyuki; Sakiyama, Ney Sussumu; Reis, Luciléa Silva dosPara analisar o uso do hipoclorito de sódio na degradação do pergaminho, como alternativa à remoção manual, bem como verificar seu efeito sobre a germinação das sementes, foram utilizadas sementes de café arábica da variedade Catuaí 44, provenientes da área experimental da Empresa Fertilizantes Heringer, no município de Manhuaçú, MG, com umidade inicial de 28,14%. Os tratamentos foram constituídos pela combinação de cinco concentrações de hipoclorito de sódio (0; 2,5; 5,0; 7,5 e 10%) e quatro tempos de embebição (6, 12, 18 e 24h), além de duas testemunhas, uma com pergaminho e outra sem pergaminho, o qual foi retirado manualmente. O experimento foi conduzido no delineamento inteiramente casualizado, em esquema fatorial 5 x 4, com 2 testemunhas adicionais, utilizando-se oito repetições de 50 sementes. Os testes utilizados para a avaliação da qualidade fisiológica das sementes foram: germinação, primeira contagem de germinação e classificação do vigor das plântulas. As concentrações de zero e 2,5% de hipoclorito de sódio não foram eficientes na retirada do pergaminho em todos os tempos de exposição. As concentrações iguais ou superiores a 5% foram efetivas na remoção do pergaminho mesmo nos menores tempos de exposição das sementes. O tratamento que proporcionou melhores resultados de germinação foi a embebição das sementes em solução de hipoclorito de sódio na concentração de 5% no tempo de 6 horas, apresentando-se superior à testemunha com pergaminho e semelhante à testemunha cujo pergaminho foi retirado manualmente. Conclui-se que a utilização do hipoclorito de sódio constitui alternativa eficiente para a retirada do pergaminho, sem causar prejuízo à germinação das sementes de café com 28,14% de umidade, podendo ser de grande utilidade na realização do teste de germinação em laboratório.