Biblioteca do Café

URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/1

Navegar

Filtros

2002 - 20042

Configurações

Autor: search.filters.author.Teixeira-Cabral, Terezinha AparecidaAssunto: search.filters.subject.Coffea

Resultados da Pesquisa

Agora exibindo 1 - 2 de 2
  • Imagem de Miniatura
    Item
    Reproducibility of the RAPD marker and its efficiency in coffee tree genotype grouping analysis
    (Crop Breeding and Applied Biotechnology, 2002) Sakiyama, Ney Sussumu; Teixeira-Cabral, Terezinha Aparecida; Zambolim, Laércio; Pereira, Antonio Alves; Barros, Everaldo Gonçalves; Sakiyama, Cássia Camargo Harger
    The genetic diversity of Coffea arabic L. cultivars is relatively narrow and its assessment and increase is important for breeding. Fifty two arbitrary primers were used to evaluate the reproducibility and the influence of the number of RAPD (Random Amplified Polymorphic DNA) markers on the estimation of genetic distances among 40 genotypes of Coffea spp. The average number of polymorphic bands was 6.69 per primer among all genotypes, and 1.27 among arabica coffee genotypes. RAPD markers were efficient in estimating the genetic distances among the genotypes. The increase in RAPD loci number during grouping analysis did not affect the major groups’ composition; however, it affected the composition of subgroups. Marker reproducibility was 76.88% and replicated data was recommended for distinguishing genotypes with the same genetic background.
  • Imagem de Miniatura
    Item
    Characterization of differential coffee tree hostsfor Hemileia vastatrix Berk. et Br. with RAPD markers
    (Crop Breeding and Applied Biotechnology, 2004-03-12) Teixeira-Cabral, Terezinha Aparecida; Sakiyama, Ney Sussumu; Zambolim, Laércio; Barros, Everaldo Gonçalves de; Silva, Dalza Gomes da
    Eighteen clones of differential coffee tree hosts for Hemileia vastatrix Berk. et Br. were characterized with RAPD markers. The genetic distances were estimated and the genealogical origin of the clones compared to data of markerbased clusters. Thirty-five primers identified 158 polymorphic loci of RAPD markers. The cluster based on the matrix of genetic dissimilarity values was compatible with information on the genealogical origin cited in literature. Specific markers for a number of clones were identified, and a combination of 12 RAPD markers allowed the characterization of the studied clones.