Biblioteca do Café
URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/1
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Item IAC Herculândia - a Coffea canephora rootstock multiresistant to Meloidogyne species(Crop Breeding and Applied Biotechnology, 2023-08-20) Guerreiro Filho, Oliveiro; Fazuoli, Luiz Carlos; Braghini, Masako Toma; Gallo, Paulo Boller; Oliveira, Claúdio Marcelo Gonçalves; Padilha, Lilian; Fatobene, Barbhara Joana dos Reis; Andrade, Vinicius Teixeira; Vasconcelos, Larissa de Brito Caixeta; Rosa, Juliana Magrinelli OsórioIAC Herculândia is a Coffea canephora cultivar multiresistant to Meloidogyne exigua, M. incognita, and M. paranaensis to be used as a rootstock for Coffea arabica cultivars. It is a synthetic cultivar resulting from recombination among the clonal cultivars IAC WG, IAC FEBS, IAC PM, IAC LCCBF, and IAC ARM.Item Morpho-agronomic and leaf anatomical traits in Coffeacanephora genotypes(Universidade Federal de Santa Maria, 2023) Silva, Larícia Olária Emerick; Schmidt, Raquel; Almeida, Rafael Nunes de; Feitoza, Rodrigo Barbosa Braga; Cunha, Maura da; Partelli, Fábio LuizGenetic variability is the basis for coffee genetic breeding. This study evaluated the potential of leaf anatomy and morpho-agronomic traits in studies of genetic variability in C. canephoracultivars. Ten genotypes were distributed in randomized block designs with three replicates. Significant differences among genotypes were detected by F-test (P < 0.05) for 13 of 15 evaluated traits. These results evidenced the heterogeneity of the studied cultivars, which is essential in composition of genetic basis in breeding programs. The Scott-Knott test detected variability among genotypes, grouped into up to four mean groups. Leaf anatomy traits presented the largest variations. Five out of seven leaf anatomy traits presented heritability higher than 80%, with emphasis on stomatal density (95.69%) and stomatal pore length (92.72%). Positive correlations were observed among morpho-agronomic and anatomic traits. Cluster analysis used the Mahalanobis general distance (D2) as a measure of genetic dissimilarity and divided the genotypes into two distinct groups. The inclusion of leaf anatomic traits to characterize C. canephoragenotypes may assist plant breeders with better genetic discrimination and with greater security in plant selection when composing cultivars.Item Caracterização molecular e estudo de autoincompatibilidade gametofítica em cafeeiros Amazônicos(Universidade Federal de Viçosa, 2024-02-26) Silva, Ana Carolina Andrade; Caixeta, Eveline Teixeira; Rocha, Rodrigo BarrosA caracterização dos materiais genéticos de café desempenha um papel crucial no desenvolvimento de variedades mais resilientes e adaptadas às condições climáticas em mudança, contribuindo para a sustentabilidade e o futuro da produção de café. Dentre as centenas de espécies do gênero Coffea já descritas, a espécie Coffea canephora se destaca por apresentar características válidas na adaptação às condições climáticas (resistência a pragas e doenças, tolerância a seca, ampla distribuição geográfica). É uma espécie diploide, alógama e que possui autoincompatibilidade gametofítica. Ampliar os estudos para melhor conhecimento desta autoincompatibilidade também se torna essencial, uma vez que para se obter cruzamentos promissores, é necessário genitores de grupos de compatibilidade diferente, para que ocorra a fertilização. Sendo assim, os objetivos do presente trabalho foram: (i) avaliar a diversidade genética de cafeeiros provenientes do programa de melhoramento da Embrapa Rondônia juntamente com genótipos de produtores locais, via marcadores moleculares SNP; (ii) usar marcadores moleculares para identificação de cafeeiros contendo diferentes genes de resistência às principais doenças da cultura: ferrugem e CBD e (iii) sequenciar e avaliar diferenças em regiões de interesse do gene S, que podem estar relacionadas ao mecanismo de autoincompatibilidade gametofítica na espécie C. canephora. Os marcadores SNPs utilizados permitiram diferenciar e caracterizar com eficiência os cafeeiros em estudo. O dendrograma pela análise destes marcadores ficou dividido em cinco grupos. O grupo I e o grupo IV alocaram dois cafeeiros cada. O grupo II alocou 33, de sua grande maioria pertencentes a variedade botânica Robusta. O grupo III ficou constituído por 18 cafeeiros, pertencentes a variedade botânica Conilon. Por fim, o quinto e maior grupo alocou 85 cafeeiros, sendo a maioria correspondente a híbridos entre as duas variedades botânicas. Marcadores moleculares ligados a genes de resistência a doenças também possibilitaram a identificação de cafeeiros contendo piramidação de alelos de resistência a Hemileia vastatrix e Colletotrichum. kahawae. Quanto ao estudo de autoincompatibilidade, as sequências de DNA dos genótipos dos diferentes grupos de autoincompatibilidade foram alinhadas e não foi encontrado polimorfismo. Ao realizar o BLAST entre a sequência consenso obtida do sequenciamento realizado com o primer HVa3 e o genoma de referência de C. canephora, observou-se que há duas regiões de alinhamento no cromossomo 2, com grande quantidade de mismatch e Gap. Esta diferença observada pode ser decorrente da ampla diversidade genética presente na espécie. Além disso, o genoma de referência disponível foi obtido de um cafeeiro geneticamente distante dos Robustas Amazônicos e Conilons cultivados no Brasil. O alinhamento entre as duas sequencias amplificadas em regiões distintas no genoma de C. canephora com o primer Hva3 mostrou serem idênticas. Este resultado pode ser um indício dessas serem regiões repetitivas dispersas no genoma da espécie. Portanto, foi possível identificar cafeeiros divergentes geneticamente, contendo a piramidação de alelos de resistência a H. vastatrix e C. kahawae. E, este estudo de autoincompatibilidade abre portas para que novos estudos possam ser realizados a partir deste. Palavraschave: Coffea canephora. Hemileia vastatrix. CBD. SNP. Gene S. Grupos de compatibilidade.Item Physiological quality of seeds of Coffea canephora from early and late clones during maturation(Instituto Agronômico (IAC), 2024-03-21) Crasque, Jeane; Brandão, Thielen Martins dos Santos; Cerri Neto, Basílio; Comério, Marcone; Volpi, Paulo Sérgio; Arantes, Lúcio de Oliveira; Machado Filho, José Altino; Milanez, Camilla Rozindo Dias; Dousseau-Arantes, SaraThe objective of this study was to evaluate the physiological quality of seeds from early and late maturing Coffea canephora clones, aiming to identify the ideal harvest time. The fruits were collected every two weeks from 188 days after anthesis (DAA) and characterized as green, cane green, cherry, raisin and dry. The seeds were evaluated for water content, germination percentage, normal and abnormal seedlings, nongerminated seeds and dead seeds, vigor index, hypocotyl lenght, and root length. Seed germination of the early maturing clone started at 202 DAA, while that of the late maturing clone started at 230 DAA, with both clones showing a water content of 63% and a dry mass of 37%. Although the optimum harvest point was identified at the cherry ripening stage, it was found that the late maturing clone showed a more pronounced variation in the maturation process, directly impacting the physiological quality of the seeds. Furthermore, a significant correlation was observed between the maturation stages and the physiological quality of the seeds. For the early maturing clone, the maximum physiological quality was recorded at 244 DAA, corresponding to 80% of fruits at the cherry stage, while for the late maturing clone this point was reached at 326 DAA, with 98% of the fruits ripe.Item Comparison of fructification efficiency between reciprocal crosses in Coffea canephora(Universidad Nacional de Colombia, 2023-10-09) Ferrão, Maria Amélia Gava; Fonseca, Aymbiré Francisco Almeida da; Ferrão, Romário Gava; Volpi, Paulo Sérgio; Verdin Filho, Abrão Carlos; Rocha, Rodrigo Barros; Riva-Souza, Elaine ManelliThis study aimed to assess and compare the behavior of superior genotypes of Coffea canephora in terms of fruiting capacity using directed hybridizations in a full diallel design with reciprocals.Hybridizations were performed using 12 possible combinations between genotypes 02, 03, 23 and 83 from the Incaper breeding program. High estimates of the genotypic coefficient of determination in terms of the total number of pinheads per cross (TNP) and fruit set (FS) indicated variability of genetic nature. FS averaged 52,27%, ranging from 31,32% to 82,09%, with the higher rates relating to clone 23 as the male parent. Differences were found between reciprocal crosses in the combinations with genotype 23, which emerged as the donor plant with the best fruiting rate, but poor receiving ability. Clone 83 prevailed as the best receiving plant and the least effective donor. In the comparison of these two genotypes, antagonism was observed in their behavior when they were used as pollen-donor plants (male parents) and pollen-receiver plants (female parents), highlighting their possible maternal effect. Crosses involving clones 02 and 03 showed no differences when used reciprocally. The estimates of genotypic correlations were negative for the four associations involving the FS characteristic, being of greater magnitude with TNP.Item Initial vegetative development and early selection of arabica coffee cultivars in a low-altitude region(Instituto Agronômico (IAC), 2023-11-10) Filla, Vinícius Augusto; Coelho, Anderson Prates; Grossi Terceiro, Matheus; Morello, Orlando Ferreira; Lemos, Leandro BorgesThe cultivation of Coffea arabica L. in a low-altitude region is a sustainable alternative for diversifying the income of farmers. On account of the variability of available cultivars, the use of indirect selection through initial vegetative development may be an auxiliary tool for identifying the most suitable genotypes for this environment. This study aimed to identify the dwarf Arabica coffee cultivars with better initial development in a low-altitude region and the morphological traits with greater relevance for the early selection of cultivars with high yield potential. The experiment was installed in the northeast of São Paulo, Brazil. The experimental design used was randomized blocks with four replications. The treatments consisted of 17 dwarf Arabica coffee cultivars. There is variability among dwarf Arabica coffee cultivars for initial vegetative development. The growth and increase in the number of nodes of the plagiotropic branch can be used in early selection to identify cultivars with greater yield potential. The cultivars IAC Obatã 4739, Obatã IAC 1669-20, and Tupi IAC 1669-33 present higher initial vegetative performance in low-altitude environments. The results obtained are useful to breeders and producers for choosing the cultivars best adapted to cultivation in this environment.Item Initial performance and genetic diversity of coffee trees cultivated under contrasting altitude conditions(Escola Superior de Agricultura "Luiz de Queiroz", 2023-08-14) Senra, João Felipe de Brites; Silva, Josimar Aleixo da; Ferreira, Adésio; Esposti, Marlon Dutra Degli; Ferrão, Maria Amélia Gava; Fassarella, Kamila Machado; Silva, Uliana Ribeiro; Milheiros, Idalina Sturião; Silva, Fernanda Gomes daThis work evaluated the initial performance and genetic diversity of Coffea canephora genotypes cultivated in environments at contrasting altitudes. Fourteen morphophysiological traits and seven descriptors of the genus Coffea spp. of coffee trees cultivated at altitudes of 140 m and 700 m were evaluated. The design used was Federer’s augmented block in a 2 × 112 factorial scheme with six blocks. The first factor was the two environments, and the second was the 112 genotypes, with eight common treatments, being five conilon coffee clones and three arabica coffee cultivars. The data were analyzed by the method of REML/BLUP and genetic correlation method. Genetic diversity was evaluated by estimating the distance matrix, applying the Gower methodology followed by the clustering method by Tocher and UPGMA. The phenotypic means were higher in the environment at an altitude of 700 m, except for plant height, number of leaves, and canopy height (CH). Genotypic effects were significant for most traits except for leaf width, CH, unit leaf area, and total leaf area. A wide genetic diversity was verified, with distances varying from 0.037 to 0.593 for the pairs of genotypes 26 × 93 and T7 × 76, respectively. Most of the traits studied showed high genotypic correlation with the environment and expressive genetic correlation between the evaluated traits thereby demonstrating the possibility of indirect selection. There is an adaptation of conilon coffee genotypes to high altitudes and the possibility of developing a specific cultivar for the southern state of Espírito Santo.Item IAC Herculândia – a Coffea canephora rootstock multiresistant to Meloidogyne species(Crop Breeding and Applied Biotechnology, 2023-08-20) Guerreiro Filho, Oliveiro; Fazuoli, Luiz Carlos; Braghini, Masako Toma; Gallo, Paulo Boller; Oliveira, Claúdio Marcelo Gonçalves; Padilha, Lilian; Fatobene, Bárbhara Joana dos Reis; Andrade, Vinicius Teixeira; Vasconcelos, Larissa de Brito Caixeta; Rosa, Juliana Magrinelli OsórioIAC Herculândia is a Coffea canephora cultivar multiresistant to Meloidogyne exigua, M. incognita, and M. paranaensis to be used as a rootstock for Coffea arabica cultivars. It is a synthetic cultivar resulting from recombination among the clonal cultivars IAC WG, IAC FEBS, IAC PM, IAC LCCBF, and IAC ARM.Item Assisted selection using molecular markers linked to rust resistance SH3 gene in Coffea arabica(Crop Breeding and Applied Biotechnology, 2023-10-25) Silva, Angelita Garbossi; Ariyoshi, Caroline; Shigueoka, Luciana Harumi; Pereira, Luiz Filipe Protasio; Sera, Gustavo HiroshiThe aim of this work was to validate markers linked to the SH3 gene of coffee leaf rust (CLR) resistance and use them in assisted selection. Initially, we validated the markers in genotypes already known to carry SH3. Next, we performed phenotype and genotype evaluation for resistance to CLR in coffee plants growing under field conditions. We used Arabica coffee progenies derived from BA-10, which carries the SH3 gene due to introgression of C. liberica. Three SCAR markers (SP-M16-SH3, BA-48-21O-f, and BA-124-12K-f) and one SSR marker (Sat244) linked to SH3 gene were used to amplify the coffee plants’ DNA. Our assessments of markers validation in resistant genotypes, SP-M16-SH3 and BA-124-12K-f, were efficient to identify the SH3 gene. These two markers were used to evaluate the progenies derived from BA-10 and were significantly linked to the phenotype evaluations. The SP-M16-SH3 marker was more efficient, with the advantage of being codominant.Item A new set of quantitative trait loci linked to lipid content in Coffea arabica(Crop Breeding and Applied Biotechnology, 2024-04-10) Muniz, Herison Victor Lima; Ariyoshi, Caroline; Ferreira, Rafaelle Vecchia; Felicio, Mariane Silva; Pereira, Luiz Filipe ProtasioLipids are compounds that play an important role in coffee bean development, contributing to beverage quality. Genome-wide association studies (GWAS) were conducted to pinpoint quantitative trait nucleotides (QTNs) linked to lipid metabolism in Coffea arabica. Genotyping by sequencing (GBS) and phenotyping data from 104 wild C. arabica accessions, Mundo Novo cultivar, and C. arabica var. Typica were utilized. GBS data were aligned to C. arabica Et039 reference genome, and both single-locus and multi-locus GWAS methods were employed. Methods were adjusted for kinship matrix, population structure, and principal component analysis. Of the 19 QTNs identified, 5 showed consistency across different population structure adjustments. The multi-locus methods mrMLM and FarmCPU proved more effective in identifying QTNs associated with lipid content. Four QTNs were situated near seven genes potentially involved in lipid metabolism. Higher frequencies of identified QTNs in accessions with elevated lipid content suggest their utility as markers for coffee plant breeding.