Biblioteca do Café
URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/1
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Resultados da Pesquisa
Item Isolation and identification of a native microbial consortium for the coffee pulp degradation above 2000 masl(Editora UFLA, 2021) Cuba, Marcela N. Arteaga; Dilas-Jiménez, Josué O.; Díaz, Norberto Villanueva; Miranda, Ofelia C. Cernaqué; Santillán, Segundo M. Tafur; García, José Enrique Olivera; Mugruza-Vassallo, Carlos A.Pulp and mucilage constitute the most abundant by-products of the processing of coffee and inadequate handling produces high-rates of pollution. Considering that in nature there is a large variety of cellulolytic microorganisms which can intervene in the degradation of coffee pulp, the inclusion of a native microbial cellulolytic consortium was assessed. The sample for isolation consisted of coffee pulp and soil from a humid forest at more than 2,000 masl. Nutrient agar was used as a means of isolation for bacteria, and malt agar for fungi, supplemented with 50% soil extract and 1% carboxylmethylbcellulose. This achieved the isolation of 118 strains of bacteria and 114 of fungi. Cellulolytic activity was established using the filter paper test, assessing and selecting only those that presented higher glucose production, among them 12 strains of fungi and 11 strains of bacteria. To obtain the microbial consortium, randomized blends were performed for both fungi and bacteria, again assessing the production of glucose. The bacterial consortium was made up of Ochrobactrum pseudo grignonense, Paenibacillus lauruscon and Bacillus xiamenensis and the fungal consortium by Fusarium sp., Penicillum sp., Cylindrocarpon sp. The optimal treatment achieved a complete degradation of the pulp in 28 days, that would contribute to the recovery and conservation of the coffee ecosystem. The main interpretation was that a wet environment at more than 2,000 masl is still suitable for coffee composting but different bacterial and fungi consortia were found to support other recent work done with one type of consortium.Item Dietary utilisation of protein and energy from fresh and ensiled coffee pulp by the nile tilapia, oreochromis niloticus(Instituto de Tecnologia do Paraná - Tecpar, 2003-03) Moreau, Yann; Arredondo, Jose-Luis; Gaime, Isabelle Perraud; Roussos, SevastianosDietary protein and energy utilisation of diets containing fresh and ensiled coffee pulp were studied on 3.2 ± 0.2 g Nile tilapia for 28 days. Diets formulation and feeding were designed on the basis of daily dietary protein and energy allowance. A control diet A (100 % protein and 100 % energy allowance) corresponding to 15 g CP kg -1 day -1 and 750 kJ kg -1 day -1 , a low protein control diet B (80 % protein and 100 % energy allowance), two diets C and E (100 % protein and 100 % energy allowance) where 20 % of protein were supplied by coffee pulp, and two diets D and F with the same amount of coffee pulp than in C and E and supplementation in non-protein energy. Inclusion of coffee pulp in the diet strongly impaired growth and feed utilisation. Silage process improved overall feed utilisation comparing to fresh coffee pulp. Results showed that fresh or ensiled coffee pulp was not a suitable feedstuff for Nile tilapia. However, better knowledge on modification occurring during silage process could allow finding the way to significantly improve nutritive value of coffee pulp by-products.Item Enhanced extraction of phenolic compounds from coffee industry’s residues through solid state fermentation by Penicillium purpurogenum(Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2015-10) García, Lady Rossana Palomino; Biasetto, Carolina Rabal; Araujo, Angela Regina; Bianchi, Vanildo Luiz DelThe use of agroindustrial residues is an economical solution to industrial biotechnology. Coffee husk and pulp are abounding residues from coffee industry which can be used as substrates in solid state fermentation process, thus allowing a liberation and increase in the phenolic compound content with high added value. By employing statistical design, initial moisture content, pH value in the medium, and the incubation temperature were evaluated, in order to increase the polyphenol content in a process of solid state fermentation by Penicillium purpurogenum. The main phenolic compounds identified through HPLC in fermented coffee residue were chlorogenic acid, caffeic acid, and rutin. Data obtained through HPLC with the radical absorbance capacity assay suggest the fermented coffee husk and pulp extracts potential as a source of phenolic acids and flavonoids. Results showed good perspectives when using P. purpurogenum strain to enhance the liberation of phenolic compounds in coffee residues.