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URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352
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Item Applying different infective structures of Beauveria bassiana to Coffea arabica infested with Hypothenemus hampei (Coleoptera: Curculionidae) under field(Instituto Biológico, 2023-01-09) Pereira, Raquel Moraes Costa; Almeida, José Eduardo Marcondes de; Batista Filho, AntonioCoffee is the most appreciated beverage in worldwide; Brazil is the largest producer and exporter of this commodity. Organochlorine endosulfan was banned from the country in 2013 due to its teratogenic agent-related features. Since then, coffee plantations have experienced increased Hypothenemus hampei infestation rates. The aim of the current study is to assess variations in the rates of Coffea arabica fruits brocaded by H. hampei after the application of entomopathogenic fungal species Beauveria bassiana IBCB66. Experiments were carried out with ‘Catuaí’ and ‘Mundo Novo’ cultivars between 2018 and 2020, during the borer transit period. Three experiments were carried out based on the application of the aforementioned fungal species on the investigated coffee plant species, both by spraying and sprinkling, at 30-day intervals; 10 fruits were collected per face of each useful plant in each repetition. The experiment has followed a randomized blocks design with five treatments, including the control, and five repetitions, each. Beauveria bassiana Ecobass (IBCB66) wettable powder spray, at the concentration of 2 × 1013·ha–1, was used in experiments I and II. On the other hand, the mix used in experiment III was prepared with blastospores at concentration of 5 × 1012·ha–1 blastospores + 0.1% Silwet. The sprinkling process in all three experiments has used dry aerial conidia at concentration of 2 × 1013·ha–1. Collected data were subjected to analysis of variance (ANOVA), which was followed by Fisher’s test at 5% probability level, in the SISVAR software. More than 35,000 fruits were assessed. In addition to variations between experiments, results have evidenced that the rate of brocaded fruits remained high.Item Efficiency of Metarhizium anisopliae in the control of Meloidogyne incognita in banana and coffee crops(Editora UFLA, 2021) Oliveira, Claudio Marcelo Gonçalves de; Rosa, Juliana Magrinelli Osório; Pillat, Rhayane Resende; Almeida, José Eduardo Marcondes deBanana and coffee are crops damaged by the root-knot nematode (Meloidogyne incognita). Thus, the objective of this research was to study the efficiency of biological products in the control of M. incognita in both crops. Four experiments were carried out in a greenhouse, in a completely randomized design, inoculating banana and coffee plants with 2000 eggs and second-stage juveniles of M. incognita. In experiment 1, each plot was constituted of a banana seedling cv. Prata Anã. The treatments, applied around each seedling, contained the fungi Metarhizium anisopliae, from the collection kept in the Biological Control/CAPSA, IB, and Pochonia chlamydosporia (the commercial product Rizotec). In experiment 2, coffee seedlings cv. Arara were treated in a single dose with M. anisopliae and P. chlamydosporia. In experiments 3 and 4, coffee seedlings cv. Mundo Novo were treated alone or mixed with M. anisopliaeand Purpureocillium lilacinum. Based on the results obtained after 150 days of inoculation, it was concluded that M. anisopliae and P. chlamydosporia have usage potential for biological control of M. incognita in bananas and coffee. In the coffee crop, P. chlamydosporia showed 81% efficiency in reducing the population of M. incognita, whereas for M. anisopliae, the efficiency was up to 76.9%. However, there was no difference between treatments with biological control agents in relation to fresh root weight and dry weight of the aerial part of the coffee plants.Item Comparision of different application methods to biological control Hypothenemus hampei(Editora UFLA, 2021) Pereira, Raquel Moraes Costa; Almeida, José Eduardo Marcondes de; Batista Filho, AntonioThere are several studies that deal with the problem of infestation by Hypothenemus hampei in Coffeea arabica. However, the ways of applying products based on entomopathogenic fungi are the same as those used for chemicals. The objective of the research was to comparison different methods of application of Beauveria bassiana against H. hampei. The experiments were developed in laboratory, with blastospores and B. bassiana airborne conidia powders were carried out. Application methods based on immersion, Potter Spray Tower and walking on treated surface. Were three experiments were carried out for each method. Bioassays’design comprised seven treatments (including the control), with five repetitions (each) and 25 beetles per repetition. Suspensions were standardized at 2.4×109, 1×109, 5×108, 1×108, 5×107 and 1×107conidia.mL-1. Conidia powder presented initial concentration of 1.14×1010and it was standardized at 1.14×109; 5.7×109; 1.14×108; 5.7×107; 1.14×107 and 5.7×106 conidia.g-1. A simultaneous test was also carried out based to the three application methods; treatment comprised conidia powder concentration of 1x108 and the control. Data were subjected to analysis of variance, which was followed by Tukey test (P<0.05) and, CL50, in the SPSS 12.1 software. The mortality better rates of H. hampei exposed to B. bassiana IBCB66 were1×108(immersion), 1×109 (Potter Spray Tower) and 1×108(walking), where as mean LC50were 6.1×108, 4.1×108, 2.4×108conidia.mL-1for each method, respectively. The highest mortality rate was recorded in the walking tests on the conidian powder at a concentration of 1×108 conidia.g-1 for the three repetitions of the method and also in the simultaneous test.