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URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352
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Item Effect of entomopathogenic fungi on the control of the coffee berry borer in the laboratory(Instituto Biológico, 2023-10-03) Ferreira, Juliana Silva; Andaló, Vanessa; Fuga, Cícero Augusto Guimarães; Sousa, Thaigoru Soares de; Soares, Carlos Marcelo SilveiraAmong the management and control tactics of the coffee berry borer Hypothenemus hampei (Ferrari), there is the use of entomopathogenic fungi. Due to the importance of prospecting isolates of entomopathogenic fungi for the control of the coffee berry borer, the objective of this study was to evaluate the efficiency of 26 isolates of entomopathogenic fungi in the control of this insect pest in the laboratory. The coffee berry borers were immersed in a solution adjusted to the concentration of 1 to 3 × 108 conidia/mL of each isolate and the control treatment (sterilized water). After seven days total mortality and confirmed mortality were evaluated. The isolates that caused the highest mortality and two commercial isolates were selected for evaluation of lethal concentration (LC50 and LC90) and lethal time (LT50 and LT90). Coffee berry borers were treated at different conidia concentrations for lethal concentration to assess total and confirmed mortality. For a lethal time, the coffee berry borers were treated at the concentration of 108 conidia/mL of the selected isolates, and, after two days and every 24 hours until the eighth day, the number of dead individuals was verified. Among the 26 isolates evaluated, 24 presented mortality higher than the control treatment, and three presented mortality higher than 85%. In the LC50 and LC90 assays, the IBCB 353 and IBCB 364 isolates were more lethal to H. hampei. In the LT50 and LT90 assays, the IBCB 66 and IBCB 353 isolates caused lethality in a shorter time.Item Botanical insecticide formulation with neem oil and D-limonene for coffee borer control(Empresa Brasileira de Pesquisa Agropecuária - Embrapa, 2021-05-12) Brito, Welington Adolfo de; Siquieroli, Ana Carolina Silva; Andaló, Vanessa; Duarte, Jéssyca Gonçalves; Sousa, Raquel Maria Ferreira de; Felisbino, John Kenedy Rodrigues Pereira; Silva, Gleidson Caetano daThe objective of this work was to evaluate the insecticidal activity of a botanical formulation with neem (Azadirachta indica) oil and D-limonene for the control of Hypothenemus hampei, as well as to determine the fatty acid composition of neem oil. Ninety-five percent neem oil was extracted from cold-pressed A. indica seeds, and D-limonene, from citrus peel. Humic extract (organic carbon), potassium, calcium, magnesium, and sulfur were included as additives. The analysis of neem oil by gas chromatography coupled to mass spectrometry showed that linoleic acid (49.28%) is the main fatty acid in its composition. Field tests were carried out on 'Topázio MG 1190' coffee (Coffea arabica) trees, with four applications of the formulated compound every 20 days for a period of 64 days. For the evaluation of insecticidal activity, the botanical formulation was applied to filter paper or topically on the insect’s dorsal side, showing a mortality of 63.34 and 100% after 48 hours, respectively. In the field, insecticidal activity caused a 62.4% reduction in the incidence of the H. hampei population, when compared with the control. The evaluated botanical formulation is efficient in controlling H. hampei in coffee plants.Item Effectiveness of coffee leaf miner control associated with spray deposition in coffee leaves(Universidade Federal de Viçosa, 2020) Zampiroli, Renan; Alvarenga, Cleyton Batista de; Rinaldi, Paula Cristina Natalino; Andaló, Vanessa; Prado, Jair Rocha do; Gonçalves, Pablo Arthur SilvaCoffee production faces challenges due to the susceptibility of the crop to insects. Owing to the coffee leaf miner Leucoptera coffeella, which is one of the main insect pests, the technology for the application of phytosanitary measures becomes the main challenge for coffee growers. This study aimed to evaluate the effectiveness of coffee leaf miner control using different methods of application. The study was conducted in an experimental site in the municipality of Monte Carmelo – Minas Gerais. Spray depositions in different positions of the plant canopy and the effective control of coffee leaf miner were evaluated using the following application treatments: a hydropneumatic sprayer with varying spray volumes (200 and 400 L ha-1), two hollow-cone spray nozzles with the presence and absence of agricultural adjuvant, and electrostatic application using a spray volume of 200 L ha-1 with and without spray adjuvant. The addition of an adjuvant to the spray did not influence coffee leaf miner control; in contrast, a spray volume of 200 L ha-1 combined with the application of coarse droplets was efficient in the management of insect pest. The reduction in the rate of the application using coarse droplets increased the operational capacity and reduced spray drift potential.Item Application technology for chemically controlling coffee leaf miner in the cerrado of Minas Gerais State(Universidade Federal Rural da Amazônia (UFRA), 2017) Zampiroli, Renan; Alvarenga, Cleyton Batista de; Andaló, Vanessa; Rinaldi, Paula Cristina; Assis, Gleice Aparecida deLeaf miner is a major coffee tree pest that causes severe losses. Most farmers perform chemical control in an attempt to reduce damage to the crop. Thus, the current study aims to analyze spraying efficiency and control effectiveness according to the management plan adopted in a coffee farm in the Cerrado of Minas Gerais. The application used Arbus sprayer, model 2,000, at the pressure of 600 kPa. The droplet spectrum was analyzed using water-sensitive paper. The plant architecture influence on the application quality was evaluated through the droplet spectrum and control efficacy. We conclude that the plant crown height and depth affected application efficiency. Spraying was an effective phytosanitary control of coffee leaf miner, reaching 87.5% control efficiency ratio, considering that infestation was three times higher than that recommended for the applied pesticide at the time of application.Item Association of Heterorhabditis amazonensis and Beauveria bassiana aiming the control of Hypothenemus hampei(Editora UFLA, 2020) Moreira, Igor Bitencourt Lima; Andaló, Vanessa; Assis, Gleice Aparecida de; Faria, Lucas Silva de; Carvalho, Fabio Janoni; Zampiroli, RenanAmong the main problems for coffee production, it is possible to mention the occurrence of coffee borer, Hypothenemus hampei. In order to seek alternatives to control the pest, the proposed objective was to evaluate the interaction between Beauveria bassiana UFU01 and Heterorhabditis amazonensis MC01 on the control of coffee borer. Therefore, four experiments were set. In the first, B. bassiana UFU01, H. amazonensis MC01 and both entomopathogens associated were applied in bored fruits displaced in vermiculite. In the second experiment, the methodology was similar to the previous one; however, the fruits were immersed in the suspensions. In the third experiment was evaluated the transport of B. bassiana UFU01 conidia by H. amazonensis, checking the mortality of Tenebrio molitor. In the fourth experiment, the fungus and the nematode were added to Petri dishes containing agar-water simultaneously to observe if the nematodes could carry B. bassiana UFU01 conidia. The obtained data were subjected to analysis of variance and the Tukey test (p <0.05), for comparison between means and regression analysis. It was found that, when entomopathogens were applied to vermiculite, treatments using B. bassiana UFU01 and H. amazonensis alone caused the highest mortality of H. hampei adults. For larvae, there was no difference among treatments. The same was observed when the fruits were dipped in the suspensions, with no difference among treatments. As for T. molitor mortality, it was observed that there was no difference among treatments. However, when applied in combination, there was a higher mortality caused by B. bassiana compared to H. amazonensis. In the last experiment, juveniles and conidia were found on the opposite side of the dish, and it can be inferred that the presence of conidia was due to transport by infective juveniles.