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URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352
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Item Enzyme Production by Induratia spp. Isolated from Coffee Plants in Brazil(Instituto de Tecnologia do Paraná - Tecpar, 2020) Monteiro, Mônica Cristina Pereira; Tavares, Dérica Gonçalves; Nery, Eduardo Mateus; Queiroz, Marisa Vieira de; Pereira, Olinto Liparini; Cardoso, Patrícia GomesEndophytic fungi belonging to the genus Muscodor now transferred to Induratia are known producers of bioactive volatile organic compounds (VOCs) with many industrial applications. However, the members of this genus have rarely been reported to produce non-volatile metabolites including enzyme. Enzymes of the endophytes are degraders of the polysaccharides available in the host plants and the knowledge of enzyme production by Induratia spp. may provide insights into their possible biotechnological applications. The aim of this study was to evaluate the activity of amylase, cellulase, lipase, pectinase, phytase, protease, endo β-1,4 glucanase and exo β-1,4 glucanase enzymes produced by fungi of the species Induratia coffeana, Induratia yucatanensis and Induratia sp. isolated from organic coffee plants. All Induratia spp. were able to produce the extracellular enzymes cellulase, pectinase, protease, and phytase. Eight fungi were able to produce lipase and four produced amylase. The specific activity of endo β-1, 4 glucanase and exo β-1,4 glucanase enzymes were detected for 9 and 8 endophytic fungi, respectively. This work demonstrated for the first time, the array of enzymes produced by Induratia spp. isolated from Coffea arabica in organic systems in Brazil.Item Phialomyces macrosporus reduces Cercospora coffeicola survival on symptomatic coffee leaves(Editora UFLA, 2019-01) Laborde, Marie Caroline Ferreira; Botelho, Deila Magna dos Santos; Rodríguez, Gabriel Alfonso Alvarez; Resende, Mário Lúcio Vilela de; Queiroz, Marisa Vieira de; Batista, Aline Duarte; Cardoso, Patrícia Gomes; Pascholati, Sérgio Florentino; Gusmão, Luis Fernando Pascholati; Martins, Samuel Júlio; Medeiros, Flávio Henrique Vasconcelos deBrown eye spot is among the most important coffee diseases, it is caused by a necrotrophic fungal Cercospora coffeicola. Saprobe fungi have potential in reducing the survival of necrotrophic pathogens and can act through competition of nutrients, mycoparasitism, antibiosis and resistance induction. We have screened saprobe fungi for the ability to reduce C. coffeicola sporulation and viability and determined the possible mechanisms involved in the biocontrol. The selected saprobe fungus, Phialomyces macrosporus, reduced the germination of C. coffeicola conidia by 40%. P. macrosporus produced both volatile and non-volatile compounds that inhibited C. coffeicola growth, sporulation and viability. The production of antimicrobial substances was the main mode of action used by the saprobe fungi. Therefore, P. macrosporus is a promising biological agent for the integrated management of brown eye spot