Periódicos
URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352
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Item Desiccation sensitivity from different coffee seed phenological stages(Associação Brasileira de Tecnologia de Sementes - ABRATES, 2014) Santos, Flávia Carvalho; Rosa, Sttela Dellyzete Veiga Franco da; Pinho, Édila Vilela de Rezende Von; Cirillo, Marcelo Angelo; Clemente, Aline da Consolação SampaioMaturity stage and drying method are the factors that most influence coffee seed quality. The objective of this study was to assess the physiological quality and investigate the electrophoretic patterns of catalase and endo-ß-mannanase enzymes and heat resistant proteins in coffee seeds harvested at different phenological stages and dried under different conditions. Physiological quality was assessed when the seeds had developed the green, greenish-yellow, cherry, overripe and dry stages after three treatments: no drying, conventional drying and fast drying. After each treatment, the physiological quality of the seeds was assessed using the germination test and electrophoretic patterns of heat resistant proteins and the activity of catalase and endo-ß-mannanase enzymes. Seeds harvested at the cherry phenological stage had the best physiological quality, and the drying process reduced quality at the cherry, overripe and dry stages. This reduction was greater under the faster drying process, but at the greenish-yellow stage, seeds had better physiological quality after slow drying. Regarding the results from electrophoretic analysis, endo-ß-mannanase and catalase activities increase as the ripeness stages advance; the activity of endo-ß-mannanase is directly associated with the deterioration process; the expression of heat resistant proteins increases with maturation process and is associated with seed physiological quality.Item Tolerance of Coffea arabica L. seeds to sub zero temperatures(Editora UFLA, 2017-05) Coelho, Stefania Vilas Boas; Rosa, Sttela Dellyzete Veiga Franco da; Clemente, Aline da Consolação Sampaio; Pereira, Cristiane Carvalho; Figueiredo, Madeleine Alves de; Reis, Leandro VilelaPreservation of the quality of coffee seeds is hindered by their intermediate behavior in storage. However, long-term storage at sub zero temperatures may be achieved by adjusting the water content of the seeds. The aim of this study was to evaluate the tolerance of coffee seeds to freezing, in relation to physiological and enzymatic modifications. Coffee seeds were dried in two manners, rapid and slow, to water contents of interest, 0.67, 0.43, 0.25, 0.18, 0.11, and 0.05 g H2O g-1 dw (dry basis). After drying, the seeds were stored at a temperature of -20 oC and of 86 oC for 24 hours and for 12 months, and then compared to seeds in cold storage at 10 oC. The seeds were evaluated through calculation of percentage of normal seedlings, percentage of seedlings with expanded cotyledonary leaves, dry matter of roots and of hypocotyls, and viability of embryos in the tetrazolium test. Expression of the enzymes superoxide dismutase, catalase, and peroxidase were evaluated by means of electrophoretic analysis. Only seeds dried more slowly to 0.18 g H2O g-1 dw present relative tolerance to storing at -20 °C for 12 months. Coffee seeds do not tolerate storage at a temperature of -86 oC for 12 months. Water contents below 0.11g H2O g-1 dw and above 0.43 g H2O g-1 dw hurt the physiological quality of coffee seeds, regardless of the type of drying, temperature, and storage period. Coffee seed embryos are more tolerant to desiccation and to freezing compared to whole seeds, especially when the seeds are dried to 0.05 g H2O g-1 dw. The catalase enzyme can be used as a biochemical marker to study tolerance to freezing in coffee seeds.