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Item Characterization and genetic diversity of Coffea canephora accessions in a germplasm bank in Espírito Santo, Brazil(Crop Breeding and Applied Biotechnology, 2021-06-20) Ferrão, Maria Amélia Gava; Mendonça, Rodolfo Ferreira de; Fonseca, Aymbiré Francisco Almeida; Ferrão, Romário Gava; Senra, João Felipe Brites; Volpi, Paulo Sérgio; Verdin Filho, Abraão Carlos; Comério, MarconeThe state of Espírito Santo is the major producer of Coffea canephora in Brazil. Knowledge of genetic reserves is fundamental to plant breeding. Therefore, the present study characterized and analyzed the genetic diversity of 600 C. canephora accessions from the germplasm bank of Incaper based on 38 traits evaluated in 24-30-month-old plants. Further, the predominant descriptors or traits were identified, and high phenotypic variability was determined. Genetic distances for the grouped (Gower), quantitative, and qualitative datasets were 0.48, 0.61, and 0.92, respectively, with accessions 76 (Conilon) and 407 (Robusta) being the most divergent ones at Incaper. In clustering using the Tocher optimization method, 30 groups were formed, with three accessions introduced from Epamig’s Robusta collection being the most dissimilar ones. Graphical dispersion analysis using the principal coordinate method revealed the predominance of three groups formed by the Robusta, Conilon, and hybrid Robusta × Conilon genotypes.Item Physiological quality of seeds of Coffea canephora from early and late clones during maturation(Instituto Agronômico (IAC), 2024-03-21) Crasque, Jeane; Brandão, Thielen Martins dos Santos; Cerri Neto, Basílio; Comério, Marcone; Volpi, Paulo Sérgio; Arantes, Lúcio de Oliveira; Machado Filho, José Altino; Milanez, Camilla Rozindo Dias; Dousseau-Arantes, SaraThe objective of this study was to evaluate the physiological quality of seeds from early and late maturing Coffea canephora clones, aiming to identify the ideal harvest time. The fruits were collected every two weeks from 188 days after anthesis (DAA) and characterized as green, cane green, cherry, raisin and dry. The seeds were evaluated for water content, germination percentage, normal and abnormal seedlings, nongerminated seeds and dead seeds, vigor index, hypocotyl lenght, and root length. Seed germination of the early maturing clone started at 202 DAA, while that of the late maturing clone started at 230 DAA, with both clones showing a water content of 63% and a dry mass of 37%. Although the optimum harvest point was identified at the cherry ripening stage, it was found that the late maturing clone showed a more pronounced variation in the maturation process, directly impacting the physiological quality of the seeds. Furthermore, a significant correlation was observed between the maturation stages and the physiological quality of the seeds. For the early maturing clone, the maximum physiological quality was recorded at 244 DAA, corresponding to 80% of fruits at the cherry stage, while for the late maturing clone this point was reached at 326 DAA, with 98% of the fruits ripe.Item Morphophysiological responses of Conilon coffee matrix plants in a super-dense clonal garden(Empresa Brasileira de Pesquisa Agropecuária - Embrapa, 2023-12-04) Silva, Josimar Aleixo da; Esposti, Marlon Dutra Degli; Senra, João Felipe de Brites; Comério, Marcone; Conceição, Amanda Oliveira da; Zacarias, Alex Justino; Milheiros, Idalina Sturião; Silva, Uliana Ribeiro; Silva, Fernanda Gomes da; Raimundo, Eduarda GonçalvesThe objective of this work was to evaluate the morphophysiological responses and cutting production of clones of Conilon coffee (Coffea canephora) cultivars in a super-dense clonal garden in the state of Espírito Santo, Brazil. The super-dense clonal garden was built in 2019 using 39 clones: 9, 9, 9, and 12 of cultivars Centenária ES8132, Diamante ES8112, ES8122 (Jequitibá), and Marilândia ES8143, respectively. The experiment was carried out in a randomized complete block design, with three replicates. Cutting production and the following morphophysiological traits were evaluated at 9 and 18 months after planting: chlorophyll index, normalized difference vegetation index, plant height, canopy height, canopy diameter, number of shoots, number of viable cuttings, number of leaves, fresh leaf mass, and plant fresh and dry matter mass. The super-dense clonal garden caused different morphophysiological responses among the studied clones. In general, clones C2, C5, C6, C8, D1, D8, D9, J8, M2, M9, M10, and M12 showed a higher mean cutting production, whereas C4, J1, J4, M4, and M5 were the most sensitive to the super-dense regime. Under these conditions, it is recommended to increase the proportion of matrix plants of the latter clones.Item Morphophysiological responses of Conilon coffee matrix plants in a super-dense clonal garden(Empresa Brasileira de Pesquisa Agropecuária - Embrapa, 2023-12-04) Silva, Josimar Aleixo da; Esposti, Marlon Dutra Degli; Senra, João Felipe de Brites; Comério, Marcone; Conceição, Amanda Oliveira da; Zacarias, Alex Justino; Milheiros, Idalina Sturião; Silva, Uliana Ribeiro; Silva, Fernanda Gomes da; Raimundo, Eduarda GonçalvesThe objective of this work was to evaluate the morphophysiological responses and cutting production of clones of Conilon coffee (Coffea canephora) cultivars in a super-dense clonal garden in the state of Espírito Santo, Brazil. The super-dense clonal garden was built in 2019 using 39 clones: 9, 9, 9, and 12 of cultivars Centenária ES8132, Diamante ES8112, ES8122 (Jequitibá), and Marilândia ES8143, respectively. The experiment was carried out in a randomized complete block design, with three replicates. Cutting production and the following morphophysiological traits were evaluated at 9 and 18 months after planting: chlorophyll index, normalized difference vegetation index, plant height, canopy height, canopy diameter, number of shoots, number of viable cuttings, number of leaves, fresh leaf mass, and plant fresh and dry matter mass. The super-dense clonal garden caused different morphophysiological responses among the studied clones. In general, clones C2, C5, C6, C8, D1, D8, D9, J8, M2, M9, M10, and M12 showed a higher mean cutting production, whereas C4, J1, J4, M4, and M5 were the most sensitive to the super-dense regime. Under these conditions, it is recommended to increase the proportion of matrix plants of the latter clones.Item Characterization and genetic diversity of Coffea canephora accessions in a germplasm bank in Espírito Santo, Brazil(Crop Breeding and Applied Biotechnology, 2021) Ferrão, Maria Amélia Gava; Mendonça, Rodolfo Ferreira de; Fonseca, Aymbiré Francisco Almeida; Ferrão, Romário Gava; Senra, João Felipe Brites; Volpi, Paulo Sérgio; Verdin Filho, Abraão Carlos; Comério, MarconeThe state of Espírito Santo is the major producer of Coffea canephora in Brazil. Knowledge of genetic reserves is fundamental to plant breeding. Therefore, the present study characterized and analyzed the genetic diversity of 600 C. canephora accessions from the germplasm bank of Incaper based on 38 traits evaluated in 24-30-month-old plants. Further, the predominant descriptors or traits were identified, and high phenotypic variability was determined. Genetic distances for the grouped (Gower), quantitative, and qualitative datasets were 0.48, 0.61, and 0.92, respectively, with accessions 76 (Conilon) and 407 (Robusta) being the most divergent ones at Incaper. In clustering using the Tocher optimization method, 30 groups were formed, with three accessions introduced from Epamig’s Robusta collection being the most dissimilar ones. Graphical dispersion analysis using the principal coordinate method revealed the predominance of three groups formed by the Robusta, Conilon, and hybrid Robusta × Conilon genotypes.Item Planococcus spp.: behavior and monitoring in conilon coffee crops(Editora UFLA, 2021) Borghi, Edinei José Armani; Fornaciari, Gabriel; Vieira, Mayara Leite; Aguiar, Ronilda Lana; Holtz, Anderson Mathias; Verdin Filho, Abraão Carlos; Comério, Marcone; Andrade Júnior, Saul de; Carvalho, José Romário deThe damage caused by the citrus mealybug attack, Planococcus spp., on Coffea canephora crops is becoming more and more frequent, and may cause losses close to 100%. Knowledge of aspects related to pest behavior and adoption of methods to monitor crop incidence are important tools for integrated pest management. Thus, the objective was to study the behavior of citrus mealybug along the phenological stages of C. canephora and to propose methods to monitor its occurrence in the crops. The study was carried out in an area consisting of adult C. canephora plants of the variety Diamante ES 8112. The study was carried out using four sampling methods: 1) soil and root sampling with a probe; 2) opening small trenches; 3) plagiotropic branches and 4) weed sampling. Evaluations were carried out monthly by checking and confirming the presence of citrus mealybug in the sampled regions. The information obtained was used to describe the behavior of citrus mealybug and to analyze the applicability of the monitoring methods studied. In the absence of the reproductive phase, citrus mealybug lodges in the root system of coffee and weeds, in the region close to the colon, moving to the aerial part of the plants (rosettes) at the beginning of the flowering of the crop. Probe sampling was not efficient, while opening small trenches is a difficult procedure and causes damage to the root system of the coffee tree. The sampling of plagiotropic branches and weeds allows the monitoring of citrus mealybug duringall the phenological phases of C. canephora.Item Implications of the cut type and apex length of stem cuttings used for the production of plantlets of Conilon coffee(Editora UFLA, 2020) Verdin Filho, Abraão Carlos; Freitas, Silvio de Jesus; Comério, Marcone; Volpi, Paulo Sérgio; Colodetti, Tafarel Victor; Rodrigues, Wagner Nunes; Fonseca, Aymbiré Francisco Almeida da; Posse, Sheila Cristina Pruculi; Fontes, Alexandre Gomes; Christo, Bruno Fardim; Vieira, Luciano Junior DiasProducing plantlets of Conilon coffee within the specific recommendations and with a high level of quality is fundamental since it is capable of promoting the initial development of the crops. To identify the best protocol to prepare the stem cuttings is fundamental to the process of plantlet production of the species. In this context, this study aimed to evaluate the implications of the type of apex cutting and the length of the remaining apex of stem cuttings to produce plantlets of Conilon coffee (Coffea canephora). To this end, two trials were conducted in the Marilândia Experimental Farm (Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural, Marilândia-ES). The first trial evaluated the types of apex cutting (straight or bevel cut), and the second trial studied the different lengths of the remaining apex (0.5, 1.0, 1.5, 2.0, and 2.5 cm). Characteristics of the vegetative growth and photosynthetic traits of the plantlets of Conilon coffee were evaluated after 120 days of cultivation in a nursery. The biomass accumulation of the plantlets of Conilon coffee produced by stem cutting may be favored by the use of bevel cut on the apex. The length of the remaining apex does not seem to have a expressive effect over the quality or growth of the plantlets, being only possible to observe effects for leaf area and biomass accumulation.