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URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352
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Item Vegetative vigor conilon coffee and its potential for in vitro callus induction(Editora UFLA, 2013-10) Santos, Maurício Reginaldo Alves dos; Ferreira, Maria das Graças Rodrigues; Oliveira, Carla Liegi Lonardoni Gomes de; Ramalho, André Rostand; Espíndula, Marcelo CuritibaConsidering the importance of the culture of Coffea canephora in the Brazilian Amazon and the need for genetic improvement of this species for cultivation in the state of Rondonia, he objective of this study was to evaluate the correlation between the initial vigor of clonal plants of C. canephora variety Conilon, under field conditions, and its potential for in vitro propagation. Promising clones belonging to the Genetic Improvement Program of Coffea sp were used Embrapa (Brazilian Agricultural Research Corporation) Rondonia. The plant height, number and length of orthotropic shoots in coffee at 10 months after planting were evaluated. In the laboratory, leaves of plants of each clone were segmented into fragments of 1 cm 2 which were inoculated on MS medium containing half the concentration of salts, AIB (10 μM), 2,4-D (20 μM) and 2iP (10 μM), sugar (20 g cm -3 ) and agar (6 g cm -3 ). Cultures were maintained in a growth chamber under 16 h photoperiod at 2000 lux and 24 ± 2 o C. The callus induction and the percentage of leaf area covered with callus cells were observed within 90 days. Clone 9 showed greater vegetative growth in the field. Only clone 12 showed no callus induced, all other clones were highly responsive to callus induction, and clone 5 showed a higher percentage of leaf area covered with callus cells. There was no phenotypic correlation between early vigor in the field and calogênico potential in vitro, for clones of coffee used.