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Item Production of Coffea canephora seedlings through cuttings in a nursery and hydroponics using different containers(Universidade Federal de Lavras, 2023-07-19) Lima, Amador Eduardo de; Castro, Elisa de Melo; Cunha, Samuel Henrique Braga da; Guimarães, Rubens José; Chalfun, Nilton Nagib Jorge; Carvalho, Alex Mendonça de; Alves, Eduardo; Carvalho, Milene Alves FigueiredoThe production of seedlings is a determining factor for the establishment and success of the coffee plantation. The objective of this study was to evaluate the production of Coffea canephora seedlings by cuttings in a modified hydroponic system and in a conventional nursery, using different containers. At the end of the experiment, growth (height, stem diameter, total number of pairs of leaves, leaf area, root area, dry matter weight of leaves, stem and root), physiological (chlorophyll a, b and total and stomatal conductance) and anatomical (stomatal density, functionality and opening) characteristics were analyzed. For statistical analysis, a completely randomized design (CRD) was used, with five treatments and six replications, with ten plants per plot. Seedlings produced in a modified hydroponic system using 50- and 120-cm³ tubettes show better vegetative growth and physiological characteristics compared to those produced in a nursery. Seedling management in a modified hydroponic system using 120-cm³ tubettes provides greater cutting survival percentage, number of remaining leaves and emission of shoots. In nurseries, the use of polyethylene bags provides better seedling quality and survival in relation to the use of tubettes, regardless of volume.Item Brown eye spot incidence during the vegetative stage of coffee grown in soil under sustainable management(Empresa Brasileira de Pesquisa Agropecuária - Embrapa, 2022-08-29) Resende, Laís Sousa; Pozza, Edson Ampélio; Luz, André Luís Faustino; Souza, Paulo Estevão de; Vilela, Marina Scalioni; Castanheira, Dalyse Toledo; Guimarães, Rubens JoséThe objective of this work was to evaluate the influence of different soil coverings, fertilizer types, and soil conditioners on the incidence and severity of brown eye spot (Cercospora coffeicola) in coffee (Coffea arabica) during the vegetative stage. The experiment was carried out in a randomized complete block design, in a 3×2×5 factorial arrangement (soil coverings × fertilizer types × soil conditioners) in subdivided plots, with three replicates. Comparisons were made among: three soil covering (plastic film, Urochloa decumbens, and no covering) in the plots; two fertililizer types (conventional and controlled release) in the sub-plots; and four soil conditioners (coffee husk, agricultural gypsum, water retention polymer, and organic compound), as well as the control, in the sub-sub-plots. The area under the progress curve for disease incidence and severity showed the lowest mean without soil covering, whereas that for number of leaves was greater with the use of the controlled-release fertilizer. The plastic film provided a greater soil moisture and a lower soil resistance penetration. The chemical composition of the organic compound reduced coffee plants growth. Soil covering with plastic film or U. decumbens favors the high incidence of brown eye spot; however, it provides a greater soil moisture and, therefore, improves the growth of coffee plants in the vegetative stage.Item Seedling production of Coffea arabica from different cultivars in a modified hydroponic system and nursery using different containers(Editora UFLA, 2021-11-22) Lima, Amador Eduardo de; Guimarães, Rubens José; Cunha, Samuel Henrique Braga da; Castro, Elisa de Melo; Carvalho, Alex Mendonça de; Faria, Mauro Magalhães LeiteThe development of modified hydroponic cultivation has aroused interest and its use has enabled advantages in several crops, but studies for coffee are still scarce. Thus, the objective of this research was to evaluate seedling production of three Coffea arabica cultivars (Mundo Novo IAC-376-4, Catuaí IAC-62 Amarelo and MGS Paraíso 2), in a modified hydroponic system, and to compare it with the nursery production system, using plastic polyethylene tubettes and bags. Coffee seedlings in the development phase with cotyledon leaves, were cultivated in both cultivation systems. At 90 days after the implementation of the experiment, the seedlings were analyzed for growth (height, stem diameter, total number, leaf area, root area, root and shoot dry matter weight), and physiological characteristics (chlorophyll content and stomatal conductance). For the statistical analysis, a completely randomized design (CRD) was used in a factorial scheme 3 (cultivars) x 2 (containers) x 2 (cultivation systems), with four replications of ten plants per plot. The innovative production system of coffee seedlings in modified hydroponics, in combination with the use of polyethylene bags, provided greater growth of coffee seedlings with better physiological characteristics. Among the cultivars tested, MGS Paraíso 2 stood out with higher seedling growth (number of leaves, stem diameter and root and shoot dry matter weight).Item Water retainer polymer in coffee plants deployment under different levels of irrigation(Editora UFLA, 2021) Resende, Thales Barcelos; Souza, Victor Hugo Silva; Castanheira, Dalyse Toledo; Carvalho, Milene Alves de Figueiredo; Guimarães, Rubens JoséThe use of water-retaining polymers may be a feasible alternative in regions with low water availability, as they has enhance the performance of coffee plants with more favorable anatomical characteristics to make physiological processes more efficient. However, the advantages of such use have been scarcely reported. Here, we studied the feasibility of using a water-retaining polymer to optimize irrigation water use during the establishment of a coffee plantation. A randomized block design was used in a factorial scheme (4 × 2) with three replications for a total of 24 experimental plots, each consisting of four pots, with one plant per unit. The experiment was installed in a greenhouse at the Coffee Science Department of the Universidade Federal de Lavras, Minas Gerais, Brazil, with four levels of irrigation (25%, 50%, 75%, and 100% of soil water at field capacity), with or without addition of the water-retaining polymer. Growth variables evaluated included: plant height, stem diameter, number of leaves, shoot and root dry weights, root volume and area. Additionally, gas exchange, leaf water potential, and leaf anatomy were analyzed. The use of water-retaining polymer during establishment of the coffee plantation ensured sufficient water supply, thus guaranteeing plant anatomical and physiological functionality and, consequently, a healthy, vigorous growth.Item Leaf anatomy, physiology and vegetative growth of fertigated Coffee arabica L. trees after exposure to pruning(Editora UFLA, 2021) Soares, Daiane dos Santos; Silva, Elisângela Aparecida da; Carvalho, Milene Alves de Figueiredo; Pereira, Fernanda Aparecida Castro; Guimarães, Rubens JoséIn coffee plants, fertigation can be an alternative way to minimize the negative effects exerted by drought and maximize fertilizer use efficiency. However, the fertilization recommendations for fertigated coffee trees are still not very specific, and the recommendations for rainfed crops are used. In addition, little is known about the nutritional requirements for fertigated coffee trees that have undergone the low recepa pruning treatment. Thus, the objective of this study was to evaluate the effects of different levels of nitrogen (N), phosphorus (P), and potassium (K) fertilizers on leaf anatomy, physiology, and veg etative growth of fertigated coffee trees (Coffea arabica L.) that have been under the low recepa pruning treatment. During the first five years of growth, the cultivar Topázio MG-1190 of the coffee crop received 10, 40, 70, 100, 130, and 160% of the fertilization levels recommended for the rainfed coffee crop. After this period, the crop was exposed to low recepa pruning. It was concluded that different doses of N, P, and K fertilizers modified the internal structure of coffee plant leaves, as well as physiological responses and plant growth; there was stronger vegetative growth, sharper leaf blade, greater thickness of spongy parenchyma, larger phloem area, and higher xylem relative hydraulic conductivity as the N, P, and K fertilizer levels in fertigated coffee (Coffea arabica L.) plants, which received the low recepa pruning treatment, increased. This knowledge can be used as a solid basis for main fertilization recommendations for fertigated coffee trees after exposure to the low recepa pruning treatment.Item Coffee genotypes morphophysiological adaptation under coffee leaf rust biotic stress(Editora UFLA, 2021) Viana, Mariana Thereza Rodrigues; Azevedo, Harianna Paula Alves de; Pereira, Fernanda Aparecida Castro; Carvalho, Milene Alves de Figueiredo; Guimarães, Rubens JoséThe identification of morphophysiological traits responsible for a better plant behavior when infected is useful for cultivar selection, and become crucial for breeding. We investigated the morphophysiological behavior of coffee genotypes before and after inoculation with the pathogen Hemileia vastatrix, causal agent of coffee rust. With multivariate techniques we identified the characteristics that most contribute to total genetic divergence of the geno types. Ten genotypes of Coffea arabica from the Germplasm Bank of Coffee from Minas Gerais were sown in a nursery and then take to a greenhouse with controlled temperature and humidity. After one month of acclimatization, the artificial inoculation with the fungus H. vastatrix was carried out. The anatomical and physiological evaluations were performed 1 day before inoculation and 160 days after inoculation. When the first symptom emerged, plants were evaluated according to a descriptive scale for coffee rust. We observed significant differences in rust severity and ostiole opening between genotypes. Different groups were formed by the K-means method, based on morphophysiological characteristics. This shows that genetic variability exists between the coffee genotypes evaluated before and after inoculation with the pathogen. The most important characteristics that contributed to the total genetic divergence were xylem vessel diameter and stomatal conductance. In conclusion, inoculation with H. vastatrix caused a change in coffee geno types based on morphophysiological characteristics.Item Phytotoxicity and leaf anatomy of young coffee plants subjected to herbicides exclusively and in associations(Editora UFLA, 2020) Rodrigues, Rafael Jorge Almeida; Gonçalves, Adenilson Henrique; Menicucci Netto, Pedro; Carneiro, Arthur Henrique Cruvinel; Castanheira, Dalyse Toledo; Guimarães, Rubens José; Carvalho, Gladyston RodriguesThe lack of work force and the damage that weeds can cause to coffee plants are the causes of the growing demand for selective herbicides to be used in coffee farming. Thus, the objective of this study was to evaluate the phytotoxicity symptoms and leaf anatomical characteristics of young coffee plants submitted to application isolated herbicides and also in associations. An experiment was carried out in a protected environment in randomized blocks: four replicates with coffee seedlings (Coffea arabica L.) cultivar “Topázio MG-1190”, grown in pots with a capacity of 11 liters of substrate. The herbicides applied, in isolation, were: pyrazosulfuron-ethyl (0.015 kg ha-1), saflufenacil (0.049 kg ha-1), imazetaphyr (0.1 kg ha-1), iodosulfuron-methyl (0.0035 kg ha-1), chlorimuron-ethyl (0.015 kg ha-1) and sethoxydim (0.184 kg ha-1). The latter was used in associations with the others. In addition, a control without herbicides was used. Phytotoxicity symptoms were evaluated up to 49 days after application (DAA) and anatomical characteristics at 65 DAA. Saflufenacil exclusively and inassociation with sethoxydim caused visual phytotoxicity symptoms in the leaves and negatively influence in the characteristics of the epidermis thickness of the adaxial face (EAD), thickness of the palisade parenchyma (PAP), thickness of the spongy parenchyma (SPP) and thickness of the mesophyll (MES). The other herbicides, isolated or in associations, didn’t cause phytotoxicity symptoms, but had negative influence in the anatomical parameters of the leaf blade. However they did not interfere with the paradermic parameters and the vascular bundle.Item Soil chemical attributes in coffee growing with different agronomic techniques(Editora UFLA, 2020) Voltolini, Giovani Belutti; Silva, Larissa Cocato da; Alecrim, Ademilson de Oliveira; Castanheira, Dalyse Toledo; Resende, Laís Sousa; Rezende, Tiago Teruel; Guimarães, Rubens JoséThe objective was to evaluate the chemical attributes in a coffee growing area, according to different agronomic techniques used and their associations. The experiment was conducted in the field, from January 2016 to October 2018. Coffee was planted in January 2016, with Mundo Novo IAC 379-19 coffee seedlings, with a spacing of 3.6 m between rows and 0.75 m between plants in the planting row. The factors under study were arranged in a 3x2x5 factorial scheme, making a total of 30 treatments. In the plots, three soil managements were randomized (soil cover with polyethylene film, soil cover with brachiaria-grass and conventional management of spontaneous vegetation). In the subplots, two types of fertilizers (conventional and increased-efficiency fertilizer). In the sub-subplots, four soil conditioners (coffee husk, phosphogypsum, water retention polymer, organic compost), and the control without conditioner. Aimed to evaluate soil chemical attributes in coffee growing with different agronomic techniques through the evaluation of: soil pH, phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), aluminum saturation (m) and base saturation (V%). The use of fertilizers combined with the application of organic compost or coffee husk, provide increased soil pH, as well as the use of ecological management of brachiaria-grass. The use of organic compost as a soil conditioner increases phosphorus availability in the soil. Coffee husk, as a soil conditioner, is an efficient potassium source for the coffee crop. The use of organic compost and phosphogypsum increases the calcium and magnesium contents in the soil. The use of organic compost and coffee husk was efficient in increasing the base saturation grown with coffee. The use of organic compost, coffee husk and phosphogypsum reduced aluminum saturation in the soil. Treatments associated with the use of organic compost improve calcium, phosphorus and magnesium levels, increase pH and base saturation, besides decreasing aluminum saturation.Item Selectivity of the herbicide chlorimuron ethyl on young coffee plants(Editora UFLA, 2019-10) Castanheira, Dalyse Toledo; Voltolini, Giovani Belutti; Alecrim, Ademilson de Oliveira; Rezende, Tiago Teruel; Menicucci Netto, Pedro; Silva, Larissa Cocato da; Guimarães, Rubens JoséThe great interference of weeds in coffee plants, due to competition for water, light and nutrients, makes their control essential. Among these control methods, the chemical stands out, due to its high efficiency and low cost. However, as a function of application failures, phytotoxicity is frequent in coffee plants due to herbicide drift. Aiming to search for active ingredients selective to coffee, the objective of this study was to evaluate the selectivity of the active ingredient Chlorimuron ethyl in coffee seedlings, and its effects on plant morphology, anatomy and physiology. The experiment was carried out in a greenhouse with ‘Topázio MG 1190’ coffee plants (Coffea arabica L.), grown in 11-L pots. The statistical design was in randomized blocks, with four replicates and four herbicide doses. Each plot consisted of three plants. The treatments were: 0%; 50%; 100% and 200% of the recommended commercial dose of the herbicide Chlorimuron ethyl. Evaluations were performed at 120 days after treatment application. Morphological, physiological and anatomical characteristics were evaluated, besides possible phytotoxicity symptoms inherent to herbicide application. The herbicide Chlorimuron ethyl caused phytotoxicity symptoms in coffee seedlings, evidenced from the leaflet dose, through leaf deformation and cracking, with growth losses not exceeding 10%, but without damage to plant physiology and anatomy.Item Phytotoxicity and growth of coffee plants as a function of the application of herbicide 2,4-D(Editora UFLA, 2019-10) Voltolini, Giovani Belutti; Castanheira, Dalyse Toledo; Silva, Larissa Cocato da; Alecrim, Ademilson de Oliveira; Rezende, Tiago Teruel; Barbosa, João Paulo Rodrigues Alves Delfino; Guimarães, Rubens JoséWeed control in coffee is of great importance for there is a great interference, standing out the competition for water, light and nutrients. Among the control methods, chemical control is highlighted as a function of its efficiency. However, the incorrect application of herbicides may imply the occurrence of drift, resulting in damage to coffee development due to phytotoxicity. In this context, the objective of this study was to evaluate the symptoms and phytotoxicity effects caused by the herbicide 2,4-D drift on the morphological, physiological and anatomical characteristics of coffee seedlings. The experiment was carried out in a greenhouse with ‘Topázio MG1190’ coffee seedlings (Coffea arabica L.), planted in 11-L pots. A randomized block design with four treatments and four replicates was used. The treatments were: 0%; 10%; 25% and 50% of the recommended commercial dose of 2,4-D (2.5 L.ha-1). Each plot consisted of three plants. The evaluations were performed at 74 days after implantation. Morphological, physiological, anatomical and phytotoxicity symptoms were evaluated as a function of the simulated herbicide drift. Coffee seedlings intoxicated by the herbicide 2,4-D presented hyponastic, chlorosis, shriveling and leaf anomalies. In the orthotropic branch, bending was observed in its extension, besides thickening in the collar region; in the plagiotropic branch, cracking was observed. Herbicide drift negatively affects the development of coffee plants. The coffee physiology was affected as a function of herbicide drift, but without damage to the leaf anatomy.