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URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352

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2004 - 20072

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Autor: search.filters.author.Sakiyama, Ney SussumuAssunto: search.filters.subject.RAPD

Resultados da Pesquisa

Agora exibindo 1 - 2 de 2
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    Characterization of differential coffee tree hostsfor Hemileia vastatrix Berk. et Br. with RAPD markers
    (Crop Breeding and Applied Biotechnology, 2004-03-12) Teixeira-Cabral, Terezinha Aparecida; Sakiyama, Ney Sussumu; Zambolim, Laércio; Barros, Everaldo Gonçalves de; Silva, Dalza Gomes da
    Eighteen clones of differential coffee tree hosts for Hemileia vastatrix Berk. et Br. were characterized with RAPD markers. The genetic distances were estimated and the genealogical origin of the clones compared to data of markerbased clusters. Thirty-five primers identified 158 polymorphic loci of RAPD markers. The cluster based on the matrix of genetic dissimilarity values was compatible with information on the genealogical origin cited in literature. Specific markers for a number of clones were identified, and a combination of 12 RAPD markers allowed the characterization of the studied clones.
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    Partial map of Coffea arabica L. and recovery of the recurrent parent in backcross progenies
    (Crop Breeding and Applied Biotechnology, 2007-06-30) Oliveira, Antonio Carlos Baião de; Sakiyama, Ney Sussumu; Caixeta, Eveline Teixeira; Zambolim, Eunize Maciel; Rufino, Raphael José Nascif; Zambolim, Laércio
    A partial map of Coffea arabica L. was constructed based on a backcross population and RAPD markers. From a total of 178 markers evaluated, only 134 that segregated 1:1 (P>0.05) were used to develop the map. Seventeen markers were not linked, while 117 formed 11 linkage groups, covering a genome distance of 803.2 cM. The maximum distance between adjacent markers was 26.9 cM, and only seven intervals exceeded 20 cM. The markers were further used for assisted selection of the plants closest to the recurrent parent, to accelerate the introgression of rust resistance genes in the coffee breeding program. Three BC1 plants resistant to coffee leaf rust and with high genetic similarity to ?Catuaí? were selected and integrated in the following backcross cycles.