Periódicos

URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352

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2021 - 20222

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Autor: search.filters.author.Santos, Maurício Reginaldo Alves dosAssunto: search.filters.subject.Genética e melhoramento

Resultados da Pesquisa

Agora exibindo 1 - 2 de 2
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    In vitro pollination and fluorescence microscopy for characterization of gametophytic selfincompatibility of Coffea canephora Pierre ex A. Froehner
    (Crop Breeding and Applied Biotechnology, 2021-11-20) Souza, Carolina Augusto de; Rocha, Rodrigo Barros; Santos, Maurício Reginaldo Alves dos; Lopes, Tatiane Almeida; Teixeira, Alexsandro Lara; Espindula, Marcelo Curitiba
    The objectives of this study were to optimize in vitro pollination and fluorescence microscopy procedures to characterize the gametophytic selfincompatibility of Coffea canephora genotypes. For that purpose, 115 in vitro pollinations were carried out among 30 parent plants of the Conilon (17) and Robusta (13) botanical varieties. Tester plants of compatibility groups I, II, and III were used as pollen grain donors. On the day of anthesis, pollen grains of the donor genotypes were transferred in vitro to one of the sides of the bifid stigma of each receptor flower. After 30 hours, the development of the pollen tubes was evaluated in fluorescence microscopy. Eleven genotypes (37%) were classified in compatibility Group I, thirteen (43%) in Group II, and six (20%) in Group III. In vitro pollination allowed reduction in the time necessary to diagnose compatibility, without exhibiting contamination caused by the presence of pollen grains of non-donor plants.
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    Expression of self-incompatibility in Coffea canephora genotypes grown in the western Amazon
    (Empresa Brasileira de Pesquisa Agropecuária - Embrapa, 2022-12-05) Depolo, Rodrigo Prado; Rocha, Rodrigo Barros; Souza, Carolina Augusto de; Santos, Maurício Reginaldo Alves dos; Espindula, Marcelo Curitiba; Teixeira, Alexsandro Lara
    The objective of this work was to characterize the expression of gametophytic self-incompatibility in a Coffea canephora breeding population, to assist in the management and development of new cultivars. For that purpose, 550 in vitro pollinations were carried out among 62 parent plants, of which 27 were from the conilon botanical variety and 35 from the robusta. Thirty-two genotypes compatible with all previously known testers were identified, suggesting the existence of new compatibility groups. From these results, hybridizations were carried out in a complete diallel design with reciprocal crosses to characterize new test plants. Based on the compatibility response with the test plants, the genotypes were clustered into the six following groups: group I, 11 (17.74%) genotypes; group II, 13 (20.97%); group III, 6 (9.68%); group IV, 9 (14.52%); group V, 8 (12.90%); and group VI, 15 (24.19%). The genotypes of the botanical variety robusta show a higher frequency of plants in compatibility group VI and a greater genetic variability, whereas those of the conilon variety have a higher frequency of plants in compatibility group II. The identification of new compatibility groups assists in new management practices that seek to increase the efficiency of pollination by favoring, through natural means, fully compatible crosses.